Photo of Amanda K. McCullough, Ph.D.

Amanda K. McCullough Ph.D.

  •      (503) 494-9958
    • Associate Professor Oregon Institute of Occupational Health Sciences
    • Associate Professor of Molecular and Medical Genetics School of Medicine
    • Co-Director, Molecular & Medical Genetics Graduate Program
    • Molecular and Medical Genetics Graduate Program School of Medicine
    • Cancer Biology Graduate Program School of Medicine
    • Program in Molecular and Cellular Biosciences School of Medicine

Dr. McCullough received her doctoral degree in Cellular and Molecular biology from the University of Vermont. She completed postdoctoral training at Oregon Health & Science University and the University of Texas Medical Branch in Galveston, Texas. Research in the McCullough laboratory is focused on the biochemical mechanisms of DNA repair systems and the regulation and roles of DNA repair in cellular responses to environmental stress. Ultimately, we are interested in correlating alterations in these systems with human cancers, aging and other disease states. Currently our research is focused on:  1) cellular pathways for the tolerance and repair of DNA-protein crosslinks; 2) biochemical mechanisms and therapeutic applications of ultraviolet (UV) light-induced DNA damage-specific glycosylases; and 3) mechanisms and regulation of human oxidative DNA damage-specific repair and how defects in these pathways contribute to colorectal cancers and metabolic disease.

Areas of interest

  • Genome Instability and Cancer
  • DNA Repair
  • Environmental Exposures and Human Disease
  • DNA Damage Response Pathways and Cancer Therapeutics
  • Ultraviolet light-induced carcinogenesis

Education

  • Ph.D., University of Vermont School of Medicine, Burlington Vermont 1992

Honors and awards

  • Excellence in Teaching – Graduate Studies Program, OHSU, 2017

Publications

  • "Modulation of UvrD helicase activity by covalent DNA-protein cross-links" Journal of Biological Chemistry July 9 2010
  • "The metabolic syndrome resulting from a knockout of the NEIL1 DNA glycosylase" Proceedings of the National Academy of Sciences of the United States of America February 15 2006
  • "hMYH cell cycle-dependent expression, subcellular localization and association with replication foci" Nucleic Acids Research July 1 2001
  • "The basal levels of 8-oxoG and other oxidative modifications in intact mitochondrial DNA are low even in repair-deficient (Ogg1-/-/Csb-/-) mice" Mutation Research December 1 2007
  • "Regulation of DNA glycosylases and their role in limiting disease" Free Radical Research April 2012
  • "BLM protein mitigates formaldehyde-induced genomic instability" DNA Repair April 1 2015
  • "Nucleotide excision repair in E. coli" Annals of the New York Academy of Sciences  1994
  • "Inhibition of DNA glycosylases via small molecule purine analogs" PLoS One December 9 2013
  • "Chlorella virus pyrimidine dimer glycosylase excises ultraviolet radiation- and hydroxyl radical-induced products 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from DNA" Photochemistry and Photobiology February 1 2002
  • "Chlorella virus PBCV-1 encodes a homolog of the bacteriophage T4 UV damage repair gene denV" Applied and Environmental Microbiology April 1997
  • "Intron conservation in a UV-specific DNA repair gene encoded by chlorella viruses" Journal of Molecular Evolution  2000
  • "Structure of T4 Pyrimidine Dimer Glycosylase in a Reduced Imine Covalent Complex with Abasic Site-containing DNA" Journal of Molecular Biology September 15 2006
  • "TAT-mediated delivery of a DNA repair enzyme to skin cells rapidly initiates repair of UV-induced DNA damage" Journal of Investigative Dermatology March 2011
  • "Initiation of base excision repair" Annual Review of Biochemistry  1999
  • "The reaction mechanism of DNA glycosylase/AP lyases at abasic sites" Biochemistry January 16 2001
  • "Cellular pathways for DNA repair and damage tolerance of formaldehyde-induced DNA-protein crosslinks" DNA Repair October 2 2009
  • "Uncoupling of nucleotide flipping and DNA bending by the T4 pyrimidine dimer DNA glycosylase" Biochemistry November 28 2006
  • "Specific interactions of t4 endonuclease v with a proposed transition state analogue for dna glycosylases" FASEB Journal  1996
  • "Minor groove orientation of the KWKK peptide tethered via the N-terminal amine to the acrolein-derived 1, N 2-γ- hydroxypropanodeoxyguanosine lesion with a trimethylene linkage" Biochemistry July 27 2010
  • "Removal of 3′-phosphoglycolate from DNA strand-break damage in an oligonucleotide substrate by recombinant human apurinic/apyrimidinic endonuclease 1" Nucleic Acids Research May 25 1994
  • "The role of base flipping in damage recognition and catalysis by T4 endonuclease V" Journal of Biological Chemistry  1997
  • "Human polymorphic variants of the NEIL1 DNA glycosylase" Journal of Biological Chemistry May 25 2007
  • "Identification of critical residues required for the mutation avoidance function of human MutY (hMYH) and implications in colorectal cancer" Cancer Letters March 8 2004
  • "Variable penetrance of metabolic phenotypes and development of high-fat diet-induced adiposity in NEIL1-deficient mice." American Journal of Physiology - Renal Fluid and Electrolyte Physiology April 2011
  • "Formaldehyde-induced genome instability is suppressed by an XPF-dependent pathway" DNA Repair March 1 2012
  • "γ-Hydroxy-1, N 2-propano-2′-deoxyguanosine DNA adduct conjugates the N-terminal amine of the KWKK peptide via a carbinolamine linkage" Chemical Research in Toxicology July 18 2011
  • "Small Molecule Inhibitors of 8-Oxoguanine DNA Glycosylase-1 (OGG1)" ACS Chemical Biology October 16 2015
  • "AP lyases and dRPases" Mutation Research - DNA Repair February 16 2000
  • "Structural determinants for specific recognition by T4 endonuclease V" Journal of Biological Chemistry  1996
  • "Modulation of the processive abasic site lyase activity of a pyrimidine dimer glycosylase" DNA Repair October 10 2011

Additional information

Edit profile