Photo of Anthony P. Barnes, Ph.D.

Anthony P. Barnes Ph.D.

    • Assistant Professor of Medicine, Division of Cardiovascular Medicine School of Medicine
    • Neuroscience Graduate Program School of Medicine
    • Program in Molecular and Cellular Biosciences School of Medicine

The Barnes Lab focuses on two fundamental cellular phenomena. One set of projects in lab is directed at understanding the nature and purpose of endothelial heterogeneity as well as how the differences in an otherwise homogenous cell type contribute to cardiac disease. Our second focus centers on the genetic and cellular mechanisms that control the development of the cerebral cortex prior to and following birth to better understand the fundamental basis of neurodevelopmental disease.

Toward our vascular biology goals, we are applying a number of cellular and molecular methodologies to understand how (cell specification mechanisms) and why (physiologic significance) the cells that form the inner lining of all blood vessels display distinct molecular identities. These differences between endothelia are often a consequence of distinct functional requirements of the organ which they serve. Our goal is to understand the genetic basis for these unique populations and how these differences may shape the response of the vasculature to disease processes and contribute to pathologic outcome in the circulatory system.

For our developmental neuroscience mission, we concentrate on how signal transduction shapes the brain patterning and neuronal maturation. The cells of the developing nervous system are bombarded by a myriad of external signals during their migration and differentiation. Deciphering how these cells interpret and respond to these signals provides insight into the mechanisms shaping brain development and responses following injury. The Barnes laboratory identifies and characterizes genes that are required for the proper formation and connectivity of the developing cerebral cortex. A major focus of the lab is directed toward mapping the pathways that link extra-cellular stimuli to alterations in gene expression and cytoskeletal rearrangement during neuronal differentiation. Neuronal polarity is a fundamental aspect of differentiation and function. In the last decade, neuroscientists have begun to discover the signaling cascades crucial for the ultimate establishment of the structural and functional distinctions of dendrites and axons. The laboratory combines an array of techniques from conditional gene deletion to cell biology and proteomics to probe the role of various signals in neuronal polarization.

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Areas of interest

  • Brain Development, Radial Glia, Neuronal Polarity, Endothelial heterogeneity, Single Cell Transcriptome/Proteome Analysis

Publications

  • "Expression of myeloid differentiation factor 88 in neurons is not requisite for the induction of sickness behavior by interleukin-1β" Journal of Neuroinflammation October 3 2012
  • "Spinophilin regulates Ca2+ signalling by binding the N-terminal domain of RGS2 and the third intracellular loop of G-protein-coupled receptors" Nature Cell Biology April 2005
  • "Phosphorylation of neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex" Neuron October 6 2005
  • "Isl1 Directly Controls a Cholinergic Neuronal Identity in the Developing Forebrain and Spinal Cord by Forming Cell Type-Specific Complexes" PLoS Genetics  2014
  • "New insights into the molecular mechanisms specifying neuronal polarity in vivo" Current Opinion in Neurobiology February 2008
  • "Establishment of axon-dendrite polarity in developing neurons" Annual Review of Neuroscience June 2009
  • "Cancer- and endotoxin-induced cachexia require intact glucocorticoid signaling in skeletal muscle" FASEB Journal September 2013
  • "Upstream flanking sequences and transcription of SINEs" Journal of Molecular Biology September 8 2000
  • "LKB1 and SAD Kinases Define a Pathway Required for the Polarization of Cortical Neurons" Cell May 4 2007
  • "Phosphodiesterase 4D forms a cAMP diffusion barrier at the apical membrane of the airway epithelium" Journal of Biological Chemistry March 4 2005
  • "The identification of a second actin-binding region in spinophilin/neurabin II" Brain Research May 19 2004
  • "Brain patterning perturbations following PTEN loss" Frontiers in Molecular Neuroscience May 14 2014
  • "Functional expression of three P2X2 receptor splice variants from guinea pig cochlea" Journal of Neurophysiology  2000
  • "STRAD pseudokinases regulate axogenesis and LKB1 stability" Neural Development March 4 2014
  • "TGF-β Signaling Specifies Axons during Brain Development" Cell July 2010
  • "Selective removal of potassium from K4In4Sb6 via laser ablation/ionization" Applied Physics Letters January 1 1995
  • "An organotypic slice culture model of chronic white matter injury with maturation arrest of oligodendrocyte progenitors" Molecular Neurodegeneration  2011
  • "Signals from the X" International Journal of Developmental Neuroscience  2002
  • "Local regulation of cystic fibrosis transmembrane regulator and epithelial sodium channel in airway epithelium." Annals of the American Thoracic Society  2004
  • "Disrupting dimerization translocates soluble epoxide hydrolase to peroxisomes" PLoS One May 1 2016
  • "Functional screening for G protein-coupled receptor targets of 14,15-epoxyeicosatrienoic acid" Prostaglandins and Other Lipid Mediators April 28 2016
  • "Specific gene expression profiles are associated with a pathologic complete response to neoadjuvant therapy in esophageal adenocarcinoma" American Journal of Surgery January 9 2017
  • "The START App" Bioinformatics January 1 2017
  • "Endothelial transcriptomics reveals activation of fibrosis-related pathways in hypertension" Physiological Genomics February 1 2018
  • "Neuronal Polarization Packs a One-Two Punch" Neuron December 5 2018
  • " Effect of thermostable mutations on the neurotensin receptor 1 (NTSR 1 ) activation state " Journal of Biomolecular Structure and Dynamics January 1 2019

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