The Gene Profiling Shared Resource (GPSR) is a unit of the Integrated Genomics Laboratory and is located on OHSU Marquam Hill Campus in Richard Jones Hall, room 5330.  We work closely with the Massively Parallel Sequencing Shared Resource and the DNA Services Core to provide support and services, from specimen processing through data generation and validation.  Training and access to many of the core's advanced technology systems is also available.

We focus on DNA and RNA isolation and evaluation, cDNA preparation, microarrays, real-time PCR and NanoString nCounter assays.

Please acknowledge support from the core in publications and presentations. 

• Suggested text: "(Insert appropriate service or platform) were performed in the OHSU Gene Profiling Shared Resource"
  • Services/Platforms: Microarray, qPCR, or NanoString nCounter assays, DNA or RNA isolation services, DNA or RNA quality assessments.
• The Gene Profiling Shared Resource receives support from the OHSU Knight Cancer Institute NCI Cancer Center Support Grant P30CA069533.

Core information flyer 

Major equipment/technologies 

• Complete Affymetrix GeneChip system for array hybridization, processing, and scanning
• Life Technologies QuantStudio qPCR System for gene expression, genotyping, and more
• NanoString SPRINT Profiler for nCounter^® assays
• NanoDrop 1000 and 8000 spectrophotometers for nucleic acid quantification
• SpectraMax i3x Multi-Mode Microplate Reader, a cost-efficient solution for high-sensitivity, high-throughput quantification of RNA and DNA samples
• Agilent 2100 Bioanalyzer & 4200 TapeStation for RNA and DNA sizing
• QIAcube & QIAsymphony Sample Prep Robots for automated nucleic acid extractions
• QIAGEN TissueLyser LT for tissue disruption
• Eppendorf epMotion 5075 Liquid Handling Robot for sample and reagent aliquoting, and sample normalization and plating
• Suite of custom and commercial software for data QA/QC and analysis, including Affymetrix Transcriptome Analysis Console

Instrument access for trained users

• QuantStudio 12K Flex real-time PCR System
• Qiagen QIAcube Sample Prep Robots
• NanoString SPRINT Profiler
• 10x Genomics Chromium Controller
• See DNA Services Core for SpectraMax i3x plate reader

Access to these instruments may be scheduled through the GPSR iLab website. 
(Training is required prior to use). If you require more information or assistance using the iLab ordering system, contact Britt Daughtry.

Please contact Britt Daughtry or Chris Harrington for information or questions about core services.

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For RNA-Seq and DNA-Seq services, please visit the MPSSR website.

For human cell line authentication and oligo synthesis services, please visit the DNA Services Core website.

For study design and analysis support for expression, genotyping and methylation arrays, please contact the OCTRI Translational Bioinformatics Core

Assistance with design, sample preparation, and data analysis for epigenetics projects utilizing RNA-seq, DNA-seq, or Sanger sequencing is available through the KCVI Epigenetics Consortium.