Nucleic Acid Isolation

Before submitting samples to the core laboratory, please read the instructions or contact one of our staff to discuss requirements. The core team is available to consult on the specifics of sample preparation for your study.

The GPSR provides nucleic acid extraction services for a large variety of sample types, including saliva, blood, FFPE specimens, fresh/frozen tissue and cultured cells. We specialize in Qiagen based isolation methods but also utilize other methods, such as TRIzol Reagent and Zymo Research based kits, depending on project needs.

Many of the core-supported isolation procedures can be run on Qiagen automation robots available in the lab. Available platforms: 2 QIAcubes (up to 12 samples per processing run) and a QIAsymphony (24-96 samples per processing run).  For multiple samples, automation-based processing provides an additional level of reproducibility while eliminating potential operator-to-operator variation. 

The GPSR also provides support for nucleic acid sample processing to remove contaminants from RNA and DNA solutions and to concentrate samples when required.

QIAcube Isolation Robot
Automated RNA/DNA Isolation using
Qiagen column based kits
QIAsymphony Isolation Robot
High-throughput automated
RNA/DNA isolation
QIAGEN TissueLyser LT
Provides fast, effective disruption of
up to 12 samples at the same time

Nucleic acid isolation and processing services

  • DNA (fresh, frozen, FFPE)
    • GPSR low throughput DNA isolation service (1-24 samples), includes standard Quality Assessment service (UV quantification and Tapestation sizing).
    • GPSR high throughput automated DNA isolation service from cells and tissue (24+ samples) is available at low, per sample cost, includes UV quantification. 
    • GPSR high throughput automated DNA isolation service from 2ml Saliva (24+ samples) is available at low, per sample cost, includes UV quantification. 
  • RNA (fresh, frozen, FFPE, prokaryotic, eukaryotic) quality assessment
    • GPSR low throughput RNA isolation service (1-24 samples) includes standard Quality Assessment service (UV quantification and Bioanalyzer sizing).
    • GPSR high throughput automated RNA isolation service from cells and tissue (24+ samples) is available at low, per sample cost, includes standard Quality Assessment service (UV quantification and Bioanalyzer sizing)
    • GPSR high throughput automated RNA isolation service from PAXgene RNA blood tubes (24+ samples) is available at low, per sample cost, includes standard Quality Assessment service (UV quantification and Bioanalyzer sizing).  This service is also available for blood samples collected in other tube types. Please contact us for more information.
  • Processing for nucleic acid cleanup and/or concentration

Support for biofluid-specific collection protocols

PAXgene blood tubes
Genotek saliva vials

The Core is currently running RNA and DNA Isolation protocols that use the following collection tubes:

  • PreAnalytiX collection tubes for blood preservation of both RNA and DNA. 
  • DNA Genotek collection vials for oral, fecal, blood, saliva, and sputum collection.
  • GeneFiX Saliva DNA Collection vials

The Core is available to consult on optimal collection protocols.  Please contact us if we can be of assistance.