Flow Cytometry


The Flow Cytometry Core of the Oregon National Primate Research Center (ONPRC) was created during the mid 1980s in response to the AIDS epidemic and subsequent discovery of an animal model in nonhuman primates;i.e., simian AIDS or SAIDS. Although the Core started out with a Coulter EPICS C analyzer/sorter, the Flow Core is now equipped with a two Becton-Dickinson (BD) LSR-II analyzers, and a BD Aria II Special Order cell sorter.

The Flow Cytometry Core serves the OHSU West Campus community by: (1) providing, maintaining and upgrading the Flow Core's equipment;(2) training users to operate the equipment in the Flow Core;and (3) providing panel and experimental advice to users unfamiliar with flow on basic flow theory and cell staining procedures, and troubleshooting help with problems.

The Flow Cytometry Core equipment includes:

  1. BD LSR II analyzer with blue, red and violet lasers, and detectors for 18 colors.
  2. BD LSR II Special Order analyzer with blue, red, violet and green lasers, and detectors for 18 colors.
  3. BD Aria II Special Order cell sorter equipped with the hazardous materials containment, plate sorting, and the heating/refrigeration options. The Aria II has violet, blue, and red lasers, and detectors for 15 colors. It is located inside one of the VGTI's Biosafety Level 3 suites.
  4. BD FACSAria Fusion Special Order cell sorter, equipped like the Aria II, but with UV, violet, blue and red lasers, and detectors for 18 colors. It is installed inside an aerosol-capturing biosafety cabinet, within an accessible room outside of a BSL3 suite.

The analyzers are offered on a user self-serve basis after proper training of new users. Flow Core personnel can be arranged to run the Aria II cell sorter.


Andrew Sylwester, Ph.D., Head of Flow Cytometry Core 503-346-5070