Integrated Pathology Core

Retinal image with multiplex fluorescence staining: credit Jonathan Stoddard, Neuringer Laboratory

About us

The ONPRC Integrated Pathology Core (IPC), directed by Oleg Varlamov, M.D. Ph.D., provides investigators with state-of-the-art histology and light microscopy imaging and analysis through extensive expertise, services, data analysis, and access to advanced equipment. The core provides routine processing and sectioning of formalin-fixed paraffin-embedded and frozen tissues, special histological stains, next-generation RNAscope/DNAscope in situ hybridization, multiplex immunohistochemistry, digital whole-slide scanning, multi-spectral confocal microscopy, and quantitative image analysis. Imaging and microscopy capabilities include a Leica Stellaris8 spectral confocal (with a white light laser - up to 8 excitation lines from 350 to 790 nm, Acousto-Optical Beam Splitter, Image Navigator module for capturing large tissue areas, resonant scanner, four HyD S detectors, and an Okolab CO2 humidity incubator with a stage top incubator with temperature and gas control for live-cell imaging), a Leica Aperio AT2 high-capacity brightfield whole slide scanner, an Olympus VS120 fluorescence (up to 5 channels; 405, 488, 561, 633, and 750 nm) and brightfield whole slide scanner, and a Keyence BZ-X810 fluorescence microscope. Image analysis is supported by the HALO Image Analysis suite, an advanced quantitative image analysis package that supports unique tissue-specific and disease-specific applications. CellSens (Olympus Bioimaging) and ImageJ are available for basic image analysis. Core personnel are available at every stage of histology and microscopy-based experiments, from planning and training to troubleshooting and quality control.

Contact us

Director, Integrated Pathology Core
Oleg Varlamov, MD, PhD
varlamov@ohsu.edu
503-346-5377

Histology services
Rachel Dannay

dannay@ohsu.edu
503-346-5077

Shipping address
Integrated Pathology Core
Oregon National Primate Research Center
Campus Mail Code L-584
505 NW 185th Avenue
Beaverton, OR 97006

Integrated Pathology Core Services

  • Pre-project planning
  • Expert consultation
  • Method documentation
  • Publication preparation

  • Routine and customized tissue processing
  • Embedding, sectioning (including large format blocks)
  • Routine hematoxylin and eosin staining
  • Frozen sectioning
  • Custom sectioning: serial, step, large format
  • Special histochemical stains
  • Cassette and slide printing

IPC_Histology_automated stainer
  • Bench top and automated immunohistochemistry
  • Antibody optimization
  • Multiplex immunohistochemistry
  • RNAscope and DNAscope In Situ Hybridization
  • Extensive portfolio of antibodies and In situ Hybridization probes validated for use in macaques

  • Tissue processors
  • Leica cassette labeler
  • Leica slide printer, automated cover-slipper, ans auto-stainer
  • Semi-automated and manual rotary microtomes
  • Biocare IntelliPATH automated immunohistochemistry platform

  • Leica Aperio AT2 400 slide scanner (bright field)
  • Olympus VS120 bright field and fluorescent (up to 5 channels) slide scanner
  • Leica Stellaris 8 confocal microscope

  • HALO Image Analysis: HALO, HALOLink
  • Olympus CellSens
  • FIJI/ ImageJ