Integrated Pathology Core

Retinal image with multiplex fluorescence staining: credit Jonathan Stoddard, Neuringer Laboratory

About us

The Integrated Pathology Core (IPC) provides investigators with state-of-the-art histology and advanced optical light microscopy imaging and analysis through extensive expertise, services, data analysis, and access to advanced equipment. Services provided include routine histology, cryosectioning, immunohistochemistry, next-generation RNAScope/DNAScope in situ hybridization, advanced optical light (bright field and multi-spectral confocal) microscopy, digital whole-slide imaging, and quantitative image analysis. Core personnel are available at every stage of histology and microscopy-based experiments, from planning to training, troubleshooting, and quality control.

The Core has an extensive resource of tissue-based analyses and techniques including multiplexed immunohistochemical and immunofluorescence staining and RNA/DNAScope in situ hybridization. In addition to routine and automated histology equipment, advanced histology techniques are supported by a Biocare IntelliPATH automated stainer for immunohistochemistry. Imaging and microscopy capabilities include a Leica Stellaris 8 confocal, Leica Aperiao AT2 bright field slide scanner, Olympus VS120 fluorescence (up to 5 channels) and Bright field slide scanner with multiple slide inserts, and a Keyence BZ-X810 Fluorescence Microscope.  Image analysis is supported by multiple platforms including the HALO Image Analysis suite (Indica Lab, Corrales, NM), an advanced quantitative image analysis package that supports unique tissue-specific and disease-specific applications.  

Contact us

Interim Director, Integrated Pathology Core
Charles Roberts, PhD

Histology services
Rachel Dannay

Advanced Microscopy services
Sathya Srinivansan, PhD

Shipping address
Integrated Pathology Core
Oregon National Primate Research Center
Campus Mail Code L-584
505 NW 185th Avenue
Beaverton, OR 97006

Integrated Pathology Core Services

  • Pre-project planning
  • Expert consultation
  • Method documentation
  • Publication preparation
  • Routine and customized tissue processing
  • Embedding, sectioning (including large format blocks)
  • Routine hematoxylin and eosin staining
  • Frozen sectioning
  • Custom sectioning: serial, step, large format
  • Special histochemical stains
  • Cassette and slide printing
IPC_Histology_automated stainer
  • Bench top and automated immunohistochemistry
  • Antibody optimization
  • Multiplex immunohistochemistry
  • RNAscope and DNAscope In Situ Hybridization
  • Extensive portfolio of antibodies and In situ Hybridization probes validated for use in macaques
  • Tissue processors
  • Leica cassette labeler
  • Leica slide printer, automated cover-slipper, ans auto-stainer
  • Semi-automated and manual rotary microtomes
  • Biocare IntelliPATH automated immunohistochemistry platform
IPC_Imaging_Aperio whole slide scanner
  • Fluorescence imaging: confocal, wide-field, and deconvolution microscopy
  • Special fluorescence techniques: FURA-2, FRET, FRAP, and live-cell time-lapse imaging
  • Large-area image montages: color bright field or fluorescence, including 3D confocal
  • Stereology image analysis
  • Image processing, 3D rendering, movies
  • Quantitative image analysis and stereology
  • Leica Aperio AT2 400 slide scanner (bright field)
  • Olympus VS120 bright field and fluorescent (up to 5 channels) slide scanner
  • Leica Stellaris 8 confocal microscope
  • MBF Bioscience Stereology System (offline analysis)
  • HALO Image Analysis: HALO, HALOLink
  • MBF Bioscience Stereology System with StereoInvestigators and NeuroLucida
  • Olympus CellSens
  • QuPath
  • FIJI/ ImageJ