The Integrated Pathology Core (IPC) provides investigators with state-of-the-art histology and light microscopy imaging and analysis through extensive expertise, services, data analysis, and access to advanced equipment. Services provided include routine histology, cryosectioning, immunohistochemistry, next-generation RNAScope/DNAScope in situ hybridization, advanced light (bright field and multi-spectral confocal) microscopy, digital whole-slide imaging, quantitative image analysis, and laser-capture microdissection. Core personnel are available at every stage of histology and microscopy-based experiments, from planning to training, troubleshooting, and quality control.
The Unit has an extensive resource of tissue-based analyses and techniques including multiplexed immunohistochemical and immunofluorescence staining and RNAScope in situ hybridization. In addition to routine and automated histology equipment, advanced histology techniques are supported by a Biocare Intellipath automated stainer for immunohistochemistry. Imaging and microscopy capabilities include a Leica SP5 AOBS confocal, a Marianas imaging workstation (3I, Denver, CO), and ArcturusXT for laser capture microdissection. Image analysis is supported by multiple platforms including the HALO Image Analysis suite (Indica Lab, Corrales, NM), an advanced quantitative image analysis package that supports unique tissue-specific and disease-specific applications.