A full list of our assay offerings can be found above in the ETC Assays and Rates link above. Below are descriptions of the analytical platforms offered in our Core.
The ETC has a Roche cobas e411 automated immunoassay platform that currently offers 40 assays for human serum and/or plasma. A number of these assays have been validated for use in NHP serum and/ or plasma samples: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, total T3, free T3, total T4, free T4, insulin, C-peptide, DHEAS, ferritin, serum beta-CrossLaps (CTX), growth hormone, osteocalcin, AMH, and vitamin D. We also offer daily STAT testing of estradiol, progesterone, and testosterone in NHP and human subjects for ONPRC and OHSU investigators for the purpose of monitoring menstrual cycles in these subjects.
Assays for proteins or peptides without extraction are available as in-house assays or from commercial sources. In-house assays include NHP LH, NHP FSH, and NHP CG. Routine commercial assays include inhibin-B, AMH, growth hormone, prolactin, leptin, ghrelin, glucagon, IGF-1, and total or high molecular weight adiponectin. The ETC will validate additional commercial assays and perform analysis as requested by investigators.
We offer a Luminex LX-200 for simultaneous measurement of multiple hormones in a single, small-volume sample. This platform is excellent for quantifying cytokines, cell signaling molecules, and disease markers. The most commonly requested assays are 29-plex and 37-plex NHP cytokine panels. We also offer custom cytokine panels designed specifically for your experiment.
Liquid chromatography-tandem mass spectrometry
The ETC has Shimadzu Nexera-LCMS-8050 liquid chromatography-tandem triple quadrupole mass spectrometry (LC-MS/MS) and Shimadzu Nexera-LCMS-8060 LC-MS/MS systems for steroid, small molecule, and peptide quantitation. By combining ultra-high-performance liquid chromatography (UHPLC) with next-generation triple quadrupole technology offered by the LCMS-8050 and the LCMS-8060, we can provide unparalleled sensitivity, precision, and throughput for our clients. We currently provide routine, affordable assays for over 15 analytes, including steroid hormones and nicotine metabolites, and will develop methods for other targets at investigator's request. Please contact us for more information on LC-MS/MS methods and pricing.
Extraction RIA for single steroids
Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA. While many of our extraction RIAs for steroids have been replaced with LC-MS/MS assays, we still offer this platform for analysis of deoxycorticosterone and corticosterone in multiple species.