My labeling bleaches during imaging.  What do I do?

  • Mount your specimen in a medium that includes antifade reagents. 
  • Change your imaging parameters to use less excitation light for shorter periods of time.

My cells die during imaging.  How can I prevent harming cells during the imaging?

  • Reduce the frequency of taking images.
  • Reduce excitation intensity and duration.
  • Increase labeling intensity.