About

The Advanced Light Microscopy Core at the Jungers Center was established in 2009 through collaborative efforts of the University Shared Resources Program and the Departments of Neurology and Molecular Microbiology and Immunology, the Jungers Center for Neurosciences Research, and the Oregon Hearing Research Center.  Technical expertise and instrumentation was pulled into a shared resource to serve OHSU researchers in need of fluorescence microscopy.

Meet the Staff

Stefanie Kaech Petrie – Director

Stefanie puts 100% effort into developing and running programs to enhance advanced light microscopy at OHSU and serves as key consultant to neuroscientists supported through a NINDS P30 Core Center Grant.  She has more than 18 years experience with advanced instrumentation for light microscopy and was among the first neuroscientists to take up the use of GFP technology.  Her past research includes visualizing microtubule- and actin-based cytoskeletal rearrangements and membrane protein trafficking in neurons. Contact Stefanie

Crystal Chaw– Imaging specialist

Crystal is an evolutionary developmental biologist who has over 15 years of experience with immunostaining and confocal microscopy. Her light microscopy experience was refined during her undergraduate and Ph.D. training, where she used a variety of techniques to investigate early cell migration in spider and crustacean embryos. Crystal especially enjoys developing protocols for novel problems, be it specimen preparation for a microscope or an analysis pipeline for software like Bitplane Imaris. She is primarily responsible for the instruments in the Knight Cancer Research Building at the south waterfront campus. Contact Crystal

Brian Jenkins – Imaging specialist

Brian’s imaging experience spans 10+ years in the neuroscience field using several microscope modalities with a focus in live-cell imaging. Brian’s passion for microscopy began as an undergraduate in San Diego, further developed during graduate school in Oregon, and sharpened as a postdoc in Wisconsin. His main interests reside in how the neuronal cytoskeleton influences protein trafficking and neuronal morphology. In the core, he enjoys assisting researchers image their samples in a meaningful way while teaching the basics of light microscopy. Brian can usually be found at Marquam Hill with a cup of decaf in hand, but can occasionally be spotted at the South Waterfront campus. Contact Brian

Hannah Bronstein- Research assistant

Hannah is a research assistant at the ALM Core and an aspiring imaging specialist. She got her first introduction to light microscopy working on her undergraduate thesis at Reed College were she used fluorescent confocal microscopy to study the origins of the zebrafish retinal stem cell niche. Her interests in biology include regenerative stem cell biology and super resolution microscopy for imaging cells in their in vivo environment. At the ALM Core, Hannah splits her time between the South Waterfront and Marquam Hill training users on a variety of light microscopes. She also consults researchers on their data analysis problems using the 3D data analysis software Bitplane Imaris and IstoVisio syGlass. Contact Hannah

Zoe Bostick- Research assistant

Zoe is a research assistant with a lifelong curiosity in nature and a particular interest in cellular neuroscience. She previously used live-cell fluorescent imaging to investigate how kinesins contribute to modulating neuronal polarity. Here in the ALM Core, Zoe’s main focus is operating and assisting others with the AxioScanners in the Knight Cancer Research Building at the south waterfront campus. She loves seeing the various tissue types and staining patterns that are brought in for imaging. She is available afternoons Mon-Fri. Contact Zoe