Instrumentation

Equipment

  • BD Symphony Five laser, 18 parameter analyzer.
  • CytoFlex S  Four laser, 12 parameter analyzer with a 96-well plate reader.
  • BD FACSAria Fusion  cell sorter: Five laser, 18 parameter cell sorter able to sort into plates or up to four populations simultaneously.  Rapidly purifies populations that are less than 1% of the original mixture to >98% purity. Capable of 4-way sorting This instrument is located in the KCRB.
  • BD Symphony S6 cell sorter: Five laser, 18 parameter cell sorter able to sort into plates or up to four populations simultaneously.  Rapidly purifies populations that are less than 1% of the original mixture to >98% purity. Capable of 6-way sorting. This instrument is located in the KCRB.
  • Two Becton Dickinson InFlux®  cell sorters: Can sort up to eleven fluorescent probes rapidly to purify populations that are less than 1% of the original mixture to >98% purity. Able to sort into plates or up to four populations simultaneously.
  • BD Fortessa®  Four lasers and 18 parameters expands the amount of data collected from a single tube. This analyzer is optimal for experiments with limited cell numbers.
  • Miltenyi AutoMACS Pro®: magnetic bead separation allows for cell enrichment or pre-sorting prior to InFlux or Vantage use.
  • BD LSR II® three-laser system allows for rapid, simultaneous eight-color analysis.
  • BD Canto II®:  5-color system with a UV laser for Hoechst/DAPI viability or cell cycle analysis.
  • Luminex 200®: For genomic and proteomic assays including protein expression, cell signaling, genotyping and metabolic markers. Multiplex up to 100 analytes in one microplate well.
  • Beckman Coulter Vi-Cell®: Fast and cost-effective cell counter adds trypan to samples and generates images and data representing cell size distribution, concentration and viability.
  • New CyTEK Aurora 5 laser, 64 parameter spectral cytometer with 96 well plate reader.