The Flow Core offers discounted flow cytometry analysis software licenses, as well as free use of FlowJo or FCS Express on dedicated workstations at KCRB and RJH.

Details on both analysis and acquisition software associated with instruments can be found below.

We offer licenses for both FlowJo and FCS Express. The billing cycle runs from February 1st - January 31st.  FlowJo licenses are $350/year and FCS Express licenses are $400/year, prorated quarterly.

Please contact Christina Metea  with questions or license purchase requests. Current license holders will be contacted at the end of the billing cycle for renewals for the following year.

If you would like to be assistance with FlowJo, you can schedule operator time to assist in your data analysis. The Flow Core also hosts software training seminars for both beginning and advanced applications. Please contact Flow Core staff for more information.

Getting Started with FlowJo

Getting Started with FCS Express

FlowJo Licenses

Each option requires a free FlowJo Portal account in order to log into the software.

  • Single User: The license allows one user to log into FlowJo with their Portal account on up to 4 computers with an internet connection, one at a time. This user is responsible for managing the account. We stress that you be very careful which computers you choose, as you will have a maximum of 4 hardware addresses associated with your account.
  • Single Computer: The license will be associated with a computer and not a person. This would be best for a shared lab workstation and can be used by an unlimited number of users. Each user of that computer will need to sign up for a FlowJo portal profile and use it to log in.

FCS Express Licenses

FCS Express requires a free De Novo Software account in order to download the software.

The Flow and Image editions have been combined, and each software license now includes the following features:

  • Flow Cytometry
  • Image Cytometry
  • High Content Tools
  • Advanced Multi-Cycle Functionality

Frequently Asked Questions

What happens if a FlowJo or FCS Express license holder leaves the lab in the middle of the year?

The license will remain active through January 31 of the following year. Licenses can be transferred to another user within the lab at any point in the year by contacting the Flow Core.

Can I switch between license types?

Yes! At any point in the year, FlowJo licenses can be changed between Single User and Single Computer licenses.

How do I manage the hardware addresses associated with my FlowJo Single User license?

Hardware addresses can be managed through the FlowJo Portal website as described here.

Is this information still current?

These prices apply to FlowJo and FCS Express licenses from February 1, 2024 through January 31, 2025.

The following software is available on instrument workstations:


All BD analyzers and cell sorters use FACSDiva software.

FACSDiva Reference Manual

Cytek SpectroFlo

The two Cytek Auroras (Sam and Frodo) both use SpectroFlo software. SpectroFlo dongles can be checked out from the Flow Core, or purchased through Cytek's website: SpectroFlo Software Dongle

Beckman Coulter CytExpert

The Beckman Coulter CytoFlex S uses CytExpert. A copy of the software can be requested by filling out the form on the right side of this page: CytExpert Software