What is Flow Cytometry?

"Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle's relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. A flow cytometer is made up of three main systems: fluidics, optics, and electronics." -BD

Flow Cytometry Services

• Daily regiment of QC protocols and cleaning procedures to ensure our instruments are functioning within manufacturer recommended set of criteria
• Quantitative measurement of fluorescent reporters to assess the distribution of specific molecules within cell populations
• Sorting to isolate purified cell populations based on detection of specific probes such as antibodies and fluorescent proteins
• Analysis of multiple characteristics such as relative cell size, antibody binding to cell surface or intracellular biomarkers, DNA and RNA content, and fluorescent protein expression
• Functional assays to measure apoptosis, enzyme activity or calcium flux
• Cell sorting into 5-15 ml conical tubes, Eppendorf tubes or multi-well plates