Animal Models

Mouse Genetic Models

Balance beam

Researchers of the PARC utilize many genetic animal models in their work. The following mouse genetic models were developed locally and are now available to the research community.

If you are interested in obtaining one of our currently available mouse models, or a cryopreserved model, please contact Dr. Tamara Richards (aka Tamara Phillips) at phillipt@ohsu.edu.

Individual tables below:

Selectively Bred Lines/Inbred Strains Created from Selectively Bred Lines
Cryopreserved Congenic, Knockout, and Transgenic Mouse Models

Selectively Bred Lines/Inbred Strains Created from Selectively Bred Lines

Line Designation Description Developing PI
FAST-1, FAST-2, SLOW-1, SLOW-2 FAST replicate 1&2 and SLOW replicate 1&2. Lines were bred to display heightened (FAST) or reduced (SLOW) sensitivity to the locomotor activating effects of a single 2.0 g/kg IP injection of ethanol, compared to response after saline injection; all are embryo cryopreserved and available from the MMRRC, email: service@mmrrc.org Tamara Phillips
WSP-1, WSP-2, WSR-1, WSR-2 Withdrawal Seizure-Prone replicate 1&2 and Withdrawal Seizure-Resistant replicate 1&2. Lines were bred to display severe (WSP) or mild (WSR) handling-induced convulsions after withdrawal from chronic ethanol vapor inhalation; all are embryo cryopreserved and available from the MMRRC, email: service@mmrrc.org John Crabbe
WSC-1, WSC-2 Withdrawal Seizure Control replicate 1&2. Randomly bred controls bred in parallel with WSP and WSR selected lines; all are embryo cryopreserved and available from the MMRRC, email: service@mmrrc.org John Crabbe
IWSP-1, IWSP-2, IWSR-1, IWSR-2 Inbred strains derived from WSP & WSR selectively bred lines, beginning with generation S26G33-52. Fully inbred. All are embryo cryopreserved and available from The Jackson Laboratory. John Crabbe
HOT-1, HOT-2, COLD-1, COLD-2 HOT & COLD mouse lines were selectively bred for extreme (COLD) or mild (HOT) hypothermia 30-60 minutes after 3 g/kg ethanol. All are embryo cryopreserved and available from The Jackson Laboratory. John Crabbe
HDID-1, HDID-2 The HDID replicate mouse lines are being actively selectively bred for high blood ethanol concentration (BEC) after consumption of ethanol (20%) in a limited access period of 4 hours called, "Drinking in the Dark (DID)." Live mice available. John Crabbe/Angela Ozburn
iHDID-1, iHDID-2 Inbred strains derived from the HDID selectively bred lines. The iHDID-1 mice are fully inbred; inbreeding began from S26 mice. The iHDID-2 mice are partially inbred using the highest BEC individuals; inbreeding began from S37 mice. As of January 2020 iHDID-2 are 7 generations away from full inbreeding. Live mice are available. John Crabbe/Angela Ozburn
HAFT-1, LAFT-1 The High Acute Functional Tolerance (HAFT) and Low Acute Functional Tolerance (LAFT) replicate 1 mouse lines were selectively bred by Drs. Erwin & Deitrich (UCHSC, Denver, CO) to display high (HAFT) or low (LAFT) acute functional tolerance to ethanol, calculated as the difference in blood ethanol concentrations at the time of regaining the ability to stay for 1 minute on a stationary dowel (12.7mm) after two consecutive injections of ethanol (1.75 g/kg followed by 2.0 g/kg, IP). Embryos are cryopreserved and available from the MMRRC, email: service@mmrrc.org John Crabbe

FAST/SLOW, WSP/WSR, HOT/COLD, and HAFT/LAFT selections were bred bi-directionally (high vs low) over 15 or more generations for the trait indicated from the founder populations of the HS/Ibg 8-way inbred strain cross (formerly available from the Institute of Behavior Genetics, Boulder, CO). Independently- derived replicated lines were developed for all four selections. The HAFT/LAFT selection was created by Dr. V. G. Erwin (UCHSC, Denver, CO) and breeding stock of the first replicate were provided to Dr. Crabbe in 2006.

HDID selections are being bred uni-directionally (high lines only) for the trait indicated from founder populations of the HS/Npt 8-way inbred strain cross (available from Dr. R. Hitzemann, OHSU, Portland, OR). These replicated lines are at 44 and 38 generations, respectively (January 2020).

Short Term Selectively Bred (STSB) lines are periodically developed for various behavioral traits by PARC Researchers for use in gene mapping and microarray studies.

Cryopreserved Congenic, Knockout, and Transgenic Mouse Models

Strain Name Developing PI Chr:cM Mb Cryo location Cryo PI Source
CHROMOSOME 1
B6.D2-D1MIT200,150 Buck 1:73-102 152.0-176.4 OHSU Phillips sperm
B6.D2 Chr 1 ISCS6 Buck 1:92-100 172.9-176.3 OHSU Buck sperm
B6.D2-Chr1 ISCS9 Buck 1:94-100 174.4-176.3 OHSU Buck sperm
B6.D2-Chr1 ISCS12 Buck 1:92-93 172.8-174.4 OHSU Phillips sperm
B6.D2 Chr 1 MtvTy/7a (D1MIT113,291) Buck 1:93.3-101 173.6-186.4 JAX Buck sperm
B6.D2-Chr1-R4 Buck 1:78-82 172.9-177.9 OHSU Buck sperm
B6.D2-Chr1-ISCS8 Buck 1:92-94 172.9-174.0 OHSU Buck embryo; sperm
D2.B6-D1MIT200,150 Buck 1:73-102 152.0-176.4 OHSU Buck sperm
D2 GIRK KO Buck 1 169.2-173.3 OHSU Buck sperm
D2.B6 Chr 1 ISCS2 Buck 1:97-100 169-2-176.4 OHSU Buck sperm
D2.B6 Chr 1 ISCS3 Buck 1:97-94 OHSU Buck sperm
CHROMOSOME 2
B6.D2-D2MIT80,285 control Klein 2:10-86 20.9-152.5 JAX Phillips sperm
B6.D2-D2MIT80,285 congenic Klein 2:10-86 20.9-152.5 JAX Phillips sperm
CHROMOSOME 4
D2.B6-Tyrp1 (D4MIT192,327 Buck 4:6-42 31.1-86.1 OHSU Phillips sperm
D2.B6-Chr4 ISCS2 Buck 4:21.9-38.6 53.6-81 OHSU Phillips sperm
D2.B6-mpdz Buck 4:37.7-38.6 75.1-76.5 OHSU Phillips sperm
D2.B6-Chr4 ISCS4 Buck 4:6.3-37.6 31.3-75.1 OHSU Phillips sperm
D2.Mpdz transgenic A Buck 4 OHSU Buck sperm
D2.Mpdz transgenic C Buck 4 OHSU Buck sperm
*D2.Mpdz transgenic E Buck 4 OHSU Buck sperm
Mpdz KO (heterozygous) Buck 4 OHSU Buck sperm
CHROMOSOME 9
B6.D2-D9MIT90,182 Phillips 9:9-58 32.6-101.4 OHSU Phillips sperm
B6.D2-D9MIT90,18 Phillips 9:9-71 32.6-120.1 JAX Phillips sperm
D2.B6-D9MIT90,18 Phillips 9:9-71 32.6-120.1 OHSU Phillips sperm
CHROMOSOME 11
D2.B6-D11MIT78,179 Buck 11:2-52 17.8-89.6 OHSU Buck sperm
D2.B6-D11MIT82,258 Buck 11:14-65 25.9-107.5 OHSU Buck sperm
D1.D2 –D11MIT217,140 Buck 11:19-28 37.4-54 OHSU Buck sperm
129-GABR gamma 2 KO (heterozygous) Buck 11 OHSU Buck sperm
CHROMOSOME 19
D2.B6-D19MIT109,137 Buck 19:4-55.7 6.1-59 OHSU Buck sperm
D2.B6-D19MIT19,91 Buck 19:26-47 39.3-47.8 OHSU Buck sperm
*It is the D2.Mpdz transgenic E mice that were characterized for ethanol withdrawal and voluntary ethanol consumption in Milner et al., 2015 Addict Biol 20(1):143-7.