Mouse Genetic Models
Researchers of the PARC utilize many genetic animal models in their work. The following mouse genetic models were developed locally and are now available to the research community.
If you are interested in obtaining one of our currently available mouse models, or a cryopreserved model, please contact Dr. Tamara Richards (aka Tamara Phillips) at firstname.lastname@example.org.
Individual tables below:
Selectively Bred Lines/Inbred Strains Created from Selectively Bred Lines
|Line Designation||Description||Developing PI|
|FAST-1, FAST-2, SLOW-1, SLOW-2||FAST replicate 1&2 and SLOW replicate 1&2. Lines were bred to display heightened (FAST) or reduced (SLOW) sensitivity to the locomotor activating effects of a single 2.0 g/kg IP injection of ethanol, compared to response after saline injection; all are embryo cryopreserved and available from the MMRRC, email: email@example.com||Tamara Phillips|
|WSP-1, WSP-2, WSR-1, WSR-2||Withdrawal Seizure-Prone replicate 1&2 and Withdrawal Seizure-Resistant replicate 1&2. Lines were bred to display severe (WSP) or mild (WSR) handling-induced convulsions after withdrawal from chronic ethanol vapor inhalation; all are embryo cryopreserved and available from the MMRRC, email: firstname.lastname@example.org||John Crabbe|
|WSC-1, WSC-2||Withdrawal Seizure Control replicate 1&2. Randomly bred controls bred in parallel with WSP and WSR selected lines; all are embryo cryopreserved and available from the MMRRC, email: email@example.com||John Crabbe|
|IWSP-1, IWSP-2, IWSR-1, IWSR-2||Inbred strains derived from WSP & WSR selectively bred lines, beginning with generation S26G33-52. Fully inbred. All are embryo cryopreserved and available from The Jackson Laboratory.||John Crabbe|
|HOT-1, HOT-2, COLD-1, COLD-2||HOT & COLD mouse lines were selectively bred for extreme (COLD) or mild (HOT) hypothermia 30-60 minutes after 3 g/kg ethanol. All are embryo cryopreserved and available from The Jackson Laboratory.||John Crabbe|
|HDID-1, HDID-2||The HDID replicate mouse lines are being actively selectively bred for high blood ethanol concentration (BEC) after consumption of ethanol (20%) in a limited access period of 4 hours called, "Drinking in the Dark (DID)." Embryos are cryopreserved and available from the MMRRC, email: firstname.lastname@example.org.||John Crabbe/Angela Ozburn|
|iHDID-1, iHDID-2||Inbred strains derived from the HDID selectively bred lines. The iHDID-1 mice are fully inbred; inbreeding began from S26 mice. The iHDID-2 mice are partially inbred using the highest BEC individuals; inbreeding began from S37 mice. As of January 2020 iHDID-2 are 7 generations away from full inbreeding. Live mice are available for iDID-1 mice and they are currently in the process be being cryopreserved at the MMRRC; iHDID-2 embryos are cryopreserved and available from the MMRRC, email: email@example.com.||John Crabbe/Angela Ozburn|
|HAFT-1, LAFT-1||The High Acute Functional Tolerance (HAFT) and Low Acute Functional Tolerance (LAFT) replicate 1 mouse lines were selectively bred by Drs. Erwin & Deitrich (UCHSC, Denver, CO) to display high (HAFT) or low (LAFT) acute functional tolerance to ethanol, calculated as the difference in blood ethanol concentrations at the time of regaining the ability to stay for 1 minute on a stationary dowel (12.7mm) after two consecutive injections of ethanol (1.75 g/kg followed by 2.0 g/kg, IP). Embryos are cryopreserved and available from the MMRRC, email: firstname.lastname@example.org||John Crabbe|
FAST/SLOW, WSP/WSR, HOT/COLD, and HAFT/LAFT selections were bred bi-directionally (high vs low) over 15 or more generations for the trait indicated from the founder populations of the HS/Ibg 8-way inbred strain cross (formerly available from the Institute of Behavior Genetics, Boulder, CO). Independently- derived replicated lines were developed for all four selections. The HAFT/LAFT selection was created by Dr. V. G. Erwin (UCHSC, Denver, CO) and breeding stock of the ﬁrst replicate were provided to Dr. Crabbe in 2006.
HDID selections are being bred uni-directionally (high lines only) for the trait indicated from founder populations of the HS/Npt 8-way inbred strain cross (previously available from Dr. R. Hitzemann, OHSU, Portland, OR). These replicated lines are at 44 and 38 generations, respectively (January 2020).
Short Term Selectively Bred (STSB) lines are periodically developed for various behavioral traits by PARC Researchers for use in gene mapping, microarray, and genomic-related studies. Currently short-term selected lines for high and low ethanol preference (HP and LP, respectively) are being utilized by PARC investigators and are available upon request. The HP and LP mice were selected for the trait indicated from the founder population of HS-CC mice (formerly available from Dr. R. Hitzemann, OHSU, Portland, OR). The first replicate of these lines are discussed in Colville et al., 2017 (Genes Brain Behavior 16(4): 462-471), and the second replicate was completed in April 2021 utilizing identical methods.
Cryopreserved Congenic, Knockout, and Transgenic Mouse Models
|Strain Name||Developing PI||Chr:cM||Mb||Cryo location||Cryo PI||Source|
|B6.D2 Chr 1 ISCS6||Buck||1:92-100||172.9-176.3||OHSU||Buck||sperm|
|B6.D2 Chr 1 MtvTy/7a (D1MIT113,291)||Buck||1:93.3-101||173.6-186.4||JAX||Buck||sperm|
|D2 GIRK KO||Buck||1||169.2-173.3||OHSU||Buck||sperm|
|D2.B6 Chr 1 ISCS2||Buck||1:97-100||169-2-176.4||OHSU||Buck||sperm|
|D2.B6 Chr 1 ISCS3||Buck||1:97-94||OHSU||Buck||sperm|
|D2.Mpdz transgenic A||Buck||4||OHSU||Buck||sperm|
|D2.Mpdz transgenic C||Buck||4||OHSU||Buck||sperm|
|*D2.Mpdz transgenic E||Buck||4||OHSU||Buck||sperm|
|Mpdz KO (heterozygous)||Buck||4||OHSU||Buck||sperm|
|129-GABR gamma 2 KO (heterozygous)||Buck||11||OHSU||Buck||sperm|
|*It is the D2.Mpdz transgenic E mice that were characterized for ethanol withdrawal and voluntary ethanol consumption in Milner et al., 2015 Addict Biol 20(1):143-7.|