Abstract:

In the present talk, I will introduce an approach for correlated measurements of physiological, anatomical and nanoscale molecular properties in brain circuits. This approach is based on the combined use of microscope modalities such as STORM super-resolution imaging and confocal microscopy. In addition to immunolabeling-based visualization of target proteins, I will also illustrate how can one visualize even individual drug molecules in cells and in complex tissue preparations by using fluorescent small molecule probes. By exploiting these methodological, I aim to offer some new insights on the nanoscale organization of cannabinoid and dopamine signaling.