Conventional Flow Cytometry Training
The OHSU Flow Core offers trainings on any of our 5 conventional flow cytometers:
- BD Symphony - 5 Laser, 21 color configuration
- BD Fortessa - 4 Laser, 18 color configuration
- BD LSR II - 3 Laser, 8 color configuration
- BD Canto II - 3 Laser, 7 color configuration
- Beckman Coulter Cytoflex S - 4 Laser, 13 color configuration
Trainings typically consist of an initial 2-hour session where user and operator will go over general instrument maintenance, start-up and shut-down procedures, setting up experiments, and running compensation. Beads will be provided by the flow core for this training, so the user does not need to bring anything. After the initial 2-hour training, users will then schedule a follow up training that is approximately 30-minutes when they have their own cells to run on the cytometer. This second 30-minute training is to get users accustomed to how their own cells look on the cytometer, and to make sure that everybody is properly setting up their experiment.
Only 1 person can be trained at a time. No Exceptions.
Before attending training, please review the pre-training resources on the right side of the page. Operators will not go over these topics during training, so it is important that users look at these before going to training so they have a basic understanding of how flow cytometry works.
Spectral Cytometer Training
The OHSU Flow-Core also offers trainings on the CyTEK Aurora (64-color spectral cytometer). Trainings are similar to our conventional flow cytometer trainings; there will be an initial 2-hour training with beads, then an approximately 30 minute follow up training when the user has their own samples to run.
Pre-Training Resources - Conventional Flow Cytometry
Introduction to Flow Cytometry - This 12 minute video by Thermo-Fisher goes over the basics of flow cytometry. This video will go over the basic instrumentation and fluidics of cytometers, and how fluorescence is detected and measured.
Flow Cytometry Overview - This is a module based overview of flow cytometry from BD. This overview will cover the three sub-systems of all cytometers: fluidics, optics, and electronics.
Pre-Training Resources -Spectral Flow Cytometry
Flow Cytometry Overview - This is a module based overview of flow cytometry from BD. This overview will cover the three sub-systems of all cytometers: fluidics, optics, and electronics. Make sure you're familiar with the basic principles of flow cytometry before attending your training.
SpectroFlo Overview Video - A quick overview of the SpectroFlo software that is used with the Aurora spectral cytometer.
Create and Unmix an Experiment in SpectroFlow - A detailed overview of how to setup and create a new experiment in SpectroFlo.