Sample Submission

The key to a successful project is starting with high quality samples. Depending on the experiment and application (DNA methylation or chromatin analyses), investigators will submit DNA or tissues/cells. 

Below are our requirements for genomic DNA. For any questions about DNA preparation, please email epigenetics@ohsu.edu

DNA Methylation

For all methylation assays we require high-quality genomic DNA:

  • 260 / 280 = 1.8 - 2.0
  • 260 / 230 > 2

DNA specifications for each assay are reported in table below.

The Gene Profile Shared Resources (GPSR) is a great resource that supports DNA extractions and/or QA here at OHSU. The team at the GPSR works closely with our team to obtain high quality DNA suitable for our assays. DNA specifications for each DNA methylation assay are reported in the table below.

Chromatin

ChIP-seq, ATAC-seq, and Hi-C experiments start from tissues or cells. The sample requirements are dependent on the assay to be performed. Please contact epigenetics@ohsu.edu before preparing your samples.

sam loading a gel
Assay Recommended DNA Input Range Minimum concentration Maximum concentration Species Restriction FFPE Compatible
Whole Genome Bisulfite Sequencing 2-50 ng 1 ng/ul 100 ng/ul None Yes
Reduced Representation Bisulfite Sequencing 100-300 ng 15 ng/ul 100 ng/ul None Yes
TruSeq Methyl Capture EPIC Sequencing 600-1,000ng 11 ng/ul 100 ng/ul Human No
Enzymatic Methyl Sequencing 20-200 ng 1 ng/ul 100 ng/ul None Yes

Sample Drop-off

Ready to start a project? Please fill out the intake form and email it to epigenetics@ohsu.edu. We will schedule a drop off after reviewing the submission.

We are located in the CHH1 at the 14th floor.

Location

Laboratory

Center for Health & Healing 1 (CHH1)

14th Floor, Room 14102

Center for Health & Healing Building 1

Tall building with the ariel tram to its left
3303 S. Bond Avenue
Portland, Oregon 97239