Sample preparation considerations

See the Sample Preparation page of our website.

Protein gel instructions

PSR Protein Gel Recommendations 

Digestion procedures

PSR’s In-gel Digestion Procedure

Protocol for Protease Max Digestion

PSR Protocol for S-Trap micro spin column digestion

Protocol for Cell Signaling Digestion

Clean up and Enrichment Protocols

Protocol for Waters SepPak tC18

Protocol for peptide desalting using NestGroup Spin columns

Protocol for phosphoenrichment with TiO2 beads

Protocol for Cell Signaling PTMScan Ab enrichment

OHSU Proteomics Shared Resources eFASP Protocol 07-29-19

Other procedures

IP Extraction Procedure

Protocol for chloroform-methanol precipitation

Protocol for preparation of Lysis Buffer