Nuclear Magnetic Resonance (NMR) Core Facility

NMR machine with person loading a sample

Requires user training

Our NMR core facility has a Bruker 400 MHz Avance NEO NanoBay spectrometer, a powerful analytical tool routinely used for structural biology research. Housed in the Department of Chemical Physiology & Biochemistry, room number LBRB 670, the spectrometer is equipped with a broadband iProbe.

Features include

  • 24 - position Sample Case automation system.
  • Broad band iProbe.
  • Standard 1H/13C experiments (1D/2D) for monitoring reaction progress, checking purity, and determining molecular structure and concentration.
  • Advanced 2D NMR experiments (COSY, HSQC, HMBC, DEPT, NOESY/ROESY) for further structural elucidation.
  • Multi-nuclear NMR analysis (31P, 19F, 15N and other)

Wilmad Precision NMR tubes model numbers 527-PP-7 (7”) and 527-PP-8 (8”) are recommended as lower quality tubes not only result in poor spectra by introducing large spinning bands and bad line shape but also damage the probe.

User training fee

User training is carried out by Tapasree Banerji.

OHSU/PSU/UO/OSU Other Academic Non-academic/Industrial
$30/hour per research group $100/hour per person $300/hour per person

User run fee

OHSU/PSU/UO/OSU Other Academic Non-academic/Industrial
$12/hour $30/hour $100/hour

Please observe these rules when using the NMR

  • Use Wilmad Precision NMR tubes model numbers 527-PP-7 (7”) and 527-PP-8 (8”).
    • Lower-quality tubes can damage the probe and can result in poor spectra by introducing large spinning bands and bad line shape.
  • Do not use tubes that are chipped at the top and make sure caps are sealed properly.
    • Glass chips and lost caps inside the NMR require expensive professional cleaning and can result in damage to the probe
  • Do not use/attach any paper label, glue, sellotape, parafilm, Teflon tape, or any other tape to the NMR tube.
    • Any manipulation of the tubes can cause unbalanced spinning. Use an ultra-fine tip permanent marker to write a short code on the cap or at the top of the NMR tube, not lower than 3 cm from the top.
  • Fill NMR tubes with at least 600-700 µl deuterated solvent or 40mm solution height.
    • Lower amounts of solvent can result in locking and shimming errors and damage the probe.
  • Do not overfill tubes.
    • Too much solvent also makes shimming difficult.
  • Only touch the spinner in the area with the black and white tape.
    • Touching the spinner in other areas can cause debris build up which results in unbalanced spinning.
  • Do not use force to insert your sample tubes into the spinner.
    • Using force can cause the tube to break which can result in severe injuries.
  • Click “change user” in Icon NMR after sample submission.
    • Forgetting this can cause charges from the following users to be charged to your account.
  • Collect tubes after completing the experiment within 4 days.
    • Leaving tubes for prolonged times will clog up the work area and result in breaking hazards.