Recent publications and preprints

Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography. 
Kim H, Spangler CJ, Matsui A, Elferich J, Kim J, Roseborough A, Nyman M, Lahtinen TM, Gouaux E. (2025) PNAS, 122(48):e2524034122. doi: 10.1073/pnas.2524034122.

The bile acid-sensitive ion channel is gated by Ca²⁺-dependent conformational changes in the transmembrane domain
Freitas MM, Gouaux E. (2025) Nature Communications, 16(6746). doi:10.1038/s41467-025-62038-9.

Gating and noelin clustering of native Ca²⁺-permeable AMPA receptors. 
Fang C, Spangler CJ, Park J, Sheldon N, Trussell LO, Gouaux E. (2025) Nature. doi:10.1038/s41586-025-09289-0.

Generation of conformation-specific monoclonal antibodies for integral membrane proteins.
Sheldon N*, Dhandapani G*, Kim J*, Spangler CJ, Fang C, Park J, Rao P, Gouaux E. (2025) Current Protocols, 5(5);e70142 (*contributed equally). doi: 10.1002/cpz1.70142.

Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses
Matsui A*, Spangler CJ*, Elferich J, Shiozaki M, Jean N, Zhao X, Qin M, Zhong H, Yu Z, Gouaux E. (2024) eLife. 13:RP98458. (*contributed equally)

Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses
Matsui A*, Spangler CJ*, Elferich J, Shiozaki M, Jean N, Zhao X, Qin M, Zhong H, Yu Z, Gouaux E. (2024) bioRxiv. Aug5:2024.04.01.587595. Pre-print. (*contributed equally)

Structure of the human dopamine transporter and mechanisms of inhibition
Srivastava DK, Navratna V, Tosh DK, Chinn A, Sk MF, Tajkhorshid E, Jacobson KA, Gouaux E. (2024) Nature. 632(8025):672-677.

The structure of the Caenorhabditis elegans TMC-2 complex suggests roles of lipid-mediated subunit contacts in mechanosensory transduction
Clark S, Jeong H, Posert R, Goehring A, Gouaux E. (2024) Proc Natl Acad Sci U S A. Feb 20;121(8):e2314096121.

Structure of C. elegans TMC-2 complex suggests roles of lipid-mediated subunit contacts in mechanosensory transduction
Clark S, Jeong H, Posert R, Goehring A, Gouaux E. (2023) bioRxiv. Aug 17:2023.08.16.553618.  Pre-print.

Large-scale growth of C. elegans and isolation of membrane protein complexes
Clark S, Jeong H, Goehring A, Kang Y, Gouaux E. (2023) Nat Protoc. Sep;18(9):2699-2716. 

Quantitative DNA-PAINT imaging of AMPA receptors in live neurons
Youn Y, Lau GW, Lee Y, Maity BK, Gouaux E, Chung HJ, Selvin PR. (2023) Cell Rep Methods. 3(2):100408.

Purification and biochemical analysis of native AMPA receptors from three different mammalian species
Rao P, Gouaux E. (2023) PLoS One. 18(3):e0275351.

Cryo-EM structures reveal native GABA_A receptor assemblies and pharmacology
Sun C, Zhu H, Clark S, Gouaux E. (2023) Nature. Oct;622(7981):195-201.  

Selected publications

Structure of the human dopamine transporter and mechanisms of inhibition
Srivastava DK, Navratna V, Tosh DK, Chinn A, Sk MF, Tajkhorshid E, Jacobson KA, Gouaux E. (2024) Nature. 632(8025):672-677.

Hippocampal AMPA receptor assemblies and mechanism of allosteric inhibition
Yu J*, Rao P*, Clark S, Mitra J, Ha T, Gouaux E. (2021) Nature. 594:448-453. (*contributed equally)

Structure of mouse protocadherin 15 of the stereocilia tip link in complex with LHFPL5
Ge J*, Elferich J*, Goehring A, Zhao J, Schuck P, Gouaux E. (2018) eLife. 7:e38770. (*contributed equally)

Glycine receptor mechanism elucidated by electron cryo-microscopy
Du J*, Lü W*, Wu S, Cheng Y, Gouaux E. (2015) Nature. 526:224-229. (*contributed equally)

Neurotransmitter and psychostimulant recognition by the dopamine transporter
Wang KH*, Penmatsa A*, Gouaux E. (2015) Nature. 521:322-327. (*contributed equally)

X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism
Chen L, Dürr KL, Gouaux E. (2014) Science. 345:1021-1026.

NMDA receptor structures reveal subunit arrangement and pore architecture
Lee CH, Lü W, Michel JC, Goehring A, Du J, Song X, Gouaux E. (2014) Nature. 511:191-197.

X-ray structure of dopamine transporter elucidates antidepressant mechanism
Penmatsa A*, Wang KH*, Gouaux E. (2013) Nature. 503:85-90. (*contributed equally)

Molecular mechanism of ATP binding and ion channel activation in P2X receptors
Hattori M and Gouaux E. (2012) Nature. 485:207-212.

X-ray structures of LeuT in substrate-free outward-open and apo inward-open states
Krishnamurthy H and Gouaux E. (2012) Nature. 481:469-474.

Principles of activation and permeation in an anion-selective Cys-loop receptor
Hibbs RE and Gouaux E. (2011) Nature. 474:54-60.