About our lab
The Gouaux lab seeks to understand the molecular basis for signal transduction at chemical synapses in the central nervous system and at sites of mechanical-electrical transduction (MET) in hair cells of the inner ear. We strive to not only elucidate molecular structures of the neurotransmitter receptors and transporters at synapses, but we also aim to elaborate the composition and molecular organization of the MET complex. We apply a host of structural and biophysical approaches, including single particle and tomographic cryo-electron microscopy, together with fluorescence microscopy methods, biochemical methods, patch clamp electrophysiology and molecular biological manipulations.
We are particularly excited about studying native signaling complexes derived from specific brain regions, as well as the MET complexes from the mouse cochlea and utricle. Ultimately, we use molecular structures and the organization of super-molecular complexes to develop mechanisms by which to understand, in atomic detail, the biochemical principles of signal transduction at synapses and of the conversion of mechanical forces to an electrical signal at hair cells. Our work thus provides fundamental insight into brain function — signaling at synapses — and hearing — the transduction of sound into a nerve impulse.
Gouaux Lab news
Serotonin transporter–ibogaine complexes illuminate mechanisms of inhibition and transport
Coleman JA, Yang D, Zhao Z, Wen P-C, Yoshioka C, Tajkhorshid E and Gouaux E
Nature, 2019 May; 569(7754):141–145
Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM
Zhao Y, Chen S, Swensen AC, Qian WJ, Gouaux E
Science, 2019 Apr 26; 364(6438):355–362
Gating mechanisms of acid-sensing ion channels
Yoder N, Yoshioka C and Gouaux E
Nature, 2018 Mar 15; 555(7696):397–401
Mechanisms for zinc and proton inhibition of the GluN1/GluN2A NMDA receptor
Jalali-Yazdi F, Chowdhury S, Yoshioka C, Gouaux E.
Cell, 2018 Nov 29; 175(6):1520–1532.e15