Circular Dichroism Spectroscopy

AVIV 215 Series Circular Dichroism Spectrophotometer

Aviv 215 Series Circular Dichroism Spectrophotometer Image
Aviv 215 Series Circular Dichroism Spectrophotometer

Circular dichroism (CD) spectrometer is a powerful tool to compare the relative structure content and thermal stability of proteins and nucleic acid samples in solution. The instrument is currently configured to:

  • Estimate the secondary structure content of proteins or nucleic acids: CD spectra have typical shapes for alpha-helical, beta-sheet, unfolded proteins and nucleic acids.
  • Measure the thermodynamic stability of a protein or nucleic acid sample from a temperature or chaotrope mediated melting curve.
  • Demonstrate similar fold and stability of protein samples that carry point mutations, which fulfills new NIH guidelines on Transparency and Reproducibility.
  • Compare structure and thermal stability of phylogenetically-related, alternatively spliced, post-transcriptionally or post-translationally-modified protein samples.
  • Measure association or dissociation kinetics of protein-protein, protein-ligand, and nucleic acid ligand interactions, depending upon the conformational changes observed in biomolecular complexes.

Cost for using the Aviv 215 Series Circular Dichroism Spectrophotometer

Training: This process charge is for training personnel on instrument usage and theory. All new users will have to be trained. This process take about four hours and costs $500.00.

Consultations and Data Collection/Analysis: This charge is for training personnel, helping to collect, analyze and interpret data. ($125.00/hr)

Instrument Setup fees: Experiment Start-up and nitrogen purging ($50.00/use)

CD Experiment hourly use: This is the hourly rate for the instrument. A minimum time of booking is 1 hour ($25.00/hr).