Immunohistochemistry Services
The Histopathology Shared Resource provides Immuhistochemistry services which include:
- Testing and titering new antibodies, both monoclonal and polyclonal. The latter should be either a purified IgG fraction or affinity-purified. (Straight serum is not suitable for immunostaining).
- Optimization of staining to enhance detection and reduce background.
- Epitope retrieval techniques (pre-treatment with heat and/or proteases)
- Specialized secondary detection reagents
- Avidin/biotin blocking steps
- Double- and triple-antibody stains using multiple chromagens.
Automated immunohistochemistry (IHC)
Immunohistochemistry services provided by the Histopathology Shared Resource (HSR) is performed using the automated Leica Bond platform.
Anyone interested in immunohistochemistry services must:
- Choose the antibody of interest
- Provide the antibody & control tissue to the core
- *HSR does not carry antibodies in stock for general use.
- All remaining antibody will be returned to the submitter after the request is completed
- Make arrangements outside of the core for interpreting the staining
- *Pathology analysis, quantitative assessment, scoring or interpretation is not available through HSR.
Contact the Histopathology Shared Resource for more information.
Previous optimized antibodies
The Histopathology Shared Resource (HSR) has performed various Immunohistochemistry stains (IHC) for researchers and can offer some insight based on previous staining experiences. It is required to discuss any IHC submissions with the Histopathology Shared Resource team to determine and review the details of this upcoming request.
Below is a list of antibody stains performed by the core for previous projects.
- For a list of previous antibodies optimized using the Leica Bond, refer to our Automated Immunohistochemistry Services*
*Please note that the Histopathology Shared Resource does not carry any antibodies in stock. The researcher is responsible for purchasing and providing any antibody or reagent needed for their project.
It is required to discuss an IHC request with the Histopathology Shared Resource team before any submissions.
Contact us to discuss optimization of a new antibody.
Shared Resource Acknowledgement
Please remember to acknowledge use of the Histopathology Shared Resource in talks, posters, and publications which include data generated in the core lab. The use of data generated in an OHSU core facility in a grant application, progress report or publication contains the implicit understanding that the PI or authors will acknowledge the use of the OHSU core facility.