2024

Astrocyte-dependent local neurite pruning in Beat-Va neurons.

Lehmann KS, Hupp MT, Abalde-Atristain L, Jefferson A, Cheng YC, Sheehan AE, Kang Y, Freeman MR. J Cell Biol. 2025 Jan 6;224(1):e202312043. doi: 10.1083/jcb.202312043. Epub 2024 Dec 9. PMID: 39652106.

The Biology of Glia.

Stevens B, Monk KR, Freeman MR. Cold Spring Harb Perspect Biol. 2024 Sep 16:a041809. doi: 10.1101/cshperspect.a041809. Online ahead of print. PMID: 39284663.

Glial Regulation of Circuit Wiring, Firing, and Expiring in the Drosophila Central Nervous System.

Coutinho-Budd J, Freeman MR, Ackerman S. Cold Spring Harb Perspect Biol. 2024 Dec 2;16(12):a041347. doi: 10.1101/cshperspect.a041347. PMID: 38565270.

Astrocyte growth is driven by the Tre1/S1pr1 phospholipid-binding G protein-coupled receptor. 

Chen J, Stork T, Kang Y, Nardone KAM, Auer F, Farrell RJ, Jay TR, Heo D, Sheehan A, Paton C, Nagel KI, Schoppik D, Monk KR, Freeman MR. Neuron. 2024 Jan 3;112(1):93-112.e10. PMID: 38096817

2023

Glial TGFβ activity promotes neuron survival in peripheral nerves.

Lassetter AP, Corty MM, Barria R, Sheehan AE, Hill JQ, Aicher SA, Fox AN, Freeman MR.

J Cell Biol. 2023 Jan 2;222(1):e202111053.

PMID: 36399182

2022

Discoidin domain receptor regulates ensheathment, survival and caliber of peripheral axons.

Corty MM, Hulegaard AL, Hill JQ, Sheehan AE, Aicher SA, Freeman MR.

Development. 2022 Dec 1;149(23):dev200636

PMID: 36355066

Astrocytic GABA transporter controls sleep by modulating GABAergic signaling in Drosophila circadian neurons.

Chaturvedi R, Stork T, Yuan C, Freeman MR, Emery P.

Curr Biol. 2022 May 9;32(9):1895-1908.e5.

PMID: 35303417

2021

An ELISA-based method for rapid genetic screens in Drosophila.

Jay TR, Kang Y, Jefferson A, Freeman MR.

Proc Natl Acad Sci U S A. 2021 Oct 26;118(43):e2107427118.

PMID: 34686600

SARM1 signaling mechanisms in the injured nervous system

Sambashivan S, Freeman MR

Curr Opin Neurobiol. 2021 Jun 24;69:247-255

PMID: 34175654

TSG101 negatively regulates mitochondrial biogenesis in axons

Lin TH, Bis-Brewer DM, Sheehan AE, Townsend LN, Maddison DC, Züchner S, Smith GA, Freeman MR

Proc Natl Acad Sci USA 2021 May 18;118(20):e2018770118

PMID: 33972422

Astrocytes close a motor circuit critical period

Ackerman SD, Perez-Catalan NA, Freeman MR, Doe CQ

Nature 2021 Apr;592(7854):414-420

PMID: 33828296

Injury-induced inhibition of bystander neurons requires dSarm and signaling from glia

Hsu JM, Kang Y, Corty MM, Mathieson D, Peters OM, Freeman MR

Neuron 2021 Feb 3;109(3):473-487.e5

PMID: 33296670

2020

TrpML-mediated astrocyte microdomain Ca²⁺ transients regulate astrocyte-tracheal interactions

Ma Z, Freeman MR

eLife 2020 Dec 7;9:e58952

PMID: 33284108

Live-imaging of astrocyte morphogenesis and function in zebrafish neural circuits

Chen J, Poskanzer KE, Freeman MR, Monk KR

Nature Neurosci. 2020 Oct;23(10):1297-1306

PMID:32895565

2019

Polymodal nociception in Drosophila requires alternative splicing of TrpA1

Gu P, Gong J, Shang Y, Wang F, Ruppell KT, Ma Z, Sheehan AE, Freeman MR, Xiang Y

Curr Biol. 2019 Dec 2;29(23):3961-3973.e6

PMID:31735672

Neural JNK3 regulates blood flow recovery after hindlimb ischemia in mice via an Egr1/Creb1 axis

Kant S, Craige SM, Chen K, Reif MM, Learnard H, Kelly M, Caliz AD, Tran KV, Ramo K, Peters OM, Freeman M, Davis RJ, Keaney JF Jr

Nat Commun. 2019 Sep 17;10(1):4223

PMID:31530804

Glutathione S-transferase regulates mitochondrial populations in axons through increased glutathione oxidation

Smith GA, Lin TH, Sheehan AE, Van der Goes van Naters W, Neukomm LJ, Graves HK, Bis-Brewer DM, Züchner S, Freeman MR

Neuron 2019 Jul 3;103(1):52-65.e6

PMID:31101394

2018

Focal adhesion molecules regulate astrocyte morphology and glutamate transporters to suppress seizure-like behavior

Cho S, Muthukumar AK, Stork T, Coutinho-Budd JC and Freeman MR

Proc Natl Acad Sci USA 2018 Oct 30;115(44):11316-11321

PMID:30327343 

Loss of Sarm1 does not suppress motor neuron degeneration in the SOD1G93A mouse model of amyotrophic lateral sclerosis

Peters OM, Lewis EA, Osterloh JM, Weiss A, Salameh JS, Metterville J, Brown RH and Freeman MR

Hum Mol Genet. 2018 Nov 1;27(21):3761-3771

PMID:30010873

Transcription factor Pebbled/RREB1 regulates injury-induced axon degeneration

Farley JE, Burdett TC, Barria R, Neukomm LJ, Kenna KP, Landers JE and Freeman MR

Proc Natl Acad Sci USA 2018 Feb 6;115(6):1358-1363

PMID:29295933

2017

The secreted neurotrophin spätzle 3 promotes glial morphogenesis and supports neuronal survival and function

Coutinho-Budd JC, Sheehan AE and Freeman MR

Genes Dev. 2017 Oct 15;31(20):2023-2038

PMID:29138279

Axon death pathways converge on Axundead to promote functional and structural axon disassembly

Neukomm LJ, Burdett TC, Seeds AM, Hampel S, Coutinho-Budd JC, Farley JE, Wong J, Karadeniz YB, Osterloh JM, Sheehan AE and Freeman MR

Neuron 2017 Jul 5;95(1):78-91.e5

PMID:28683272

Axon degeneration induces glial responses through Draper-TRAF4-JNK signalling

Lu TY, MacDonald JM, Neukomm LJ, Sheehan AE, Bradshaw R, Logan MA and Freeman MR

Nat Commun. 2017 Feb 6;8:14355

PMID:28165006

2016

Neuromodulators signal through astrocytes to alter neural circuit activity and behaviour

Ma Z, Stork T, Bergles DE and Freeman MR

Nature 2016 Nov 17;539(7629):428-432

PMID:27828941

Prevalent presence of periodic actin-spectrin-based membrance skeleton in a broad range of neuronal cell types and animal species

He J, Zhou R, Wu Z, Carrasco MA, Kurshan PT, Farley JE, Simon DJ, Wang G, Han B, Hao J, Heller E, Freeman MR, Shen K, Maniatis T, Tessier-Lavigne M and Zhuang X

Proc Natl Acad Sci USA 2016 May 24;113(21):6029-6034

PMID:27162329

Dendrites actively restrain axon outgrowth and regeneration

Francis MM and Freeman MR

Proc Natl Acad Sci USA 2016 May 17;113(20):5465-5466

PMID:27147603

Attenuated traumatic axonal injury and improved functional outcome after traumatic brain injury in mice lacking Sarm1

Henninger N, Bouley J, Sikoglu EM, An J, Moore CM, King JA, Bowser R, Freeman MR and Brown RH Jr

Brain 2016 Apr;139(Pt 4):1094-1105

PMID:26912636

2015

Letting go of JuNK by disassembly of adhesive complexes

Farely JE and Freeman MR

Neuron 2015 Dec 2;88(5):848-850

PMID:26637791

Age-dependent TRDP-43-medicated motor neuron degeneration requires GSK3, hat-trick, and xmas-2

Sreedharan J, Neukomm LJ, Brown RH Jr and Freeman MR

Curr Biol. 2015 Aug 17;25(16):2130-2136

PMID:26234214

Drosphila central nervous system glia

Freeman MR

Cold Spring Harb Perspect Biol. 2015 Feb 26;7(11). pii: a020552

PMID:25722465

2014

PI3K signaling and Stat92E converge to modulate glial responsiveness to axonal injury

Doherty J, Sheehan AE, Bradshaw R, Fox AN, Lu TY and Freeman MR

PLoS Biol. 2014 Nov 4;12(11):e1001985

PMID:25369313

Activity-dependent regulation of astrocyte GAT levels during synaptogenesis

Muthukumar AK, Stork T and Freeman MR

Nat Neurosci. 2014 Oct;17(10):1340-1350

PMID:25151265

DRK/DOS/SOS converge with Crk/Mbc/dCed-12 to activate Rac1 during glial engulfment of axonal debris

Lu TY, Doherty J and Freeman MR

Proc Natl Acad Sci USA 2014 Aug 26;111(34):12544-12549

PMID:25099352

Astrocytes eyeball axonal mitochondria

Burdett TC and Freeman MR

Science 2014 Jul 25;345(6195):385-386

PMID:25061193

Neuron-glia interactions through the Heartless FGF receptor signaling pathway mediate morphogenesis of Drosophila astrocytes

Stork T, Sheehan A, Tasdemir-Yilmaz OE and Freeman MR

Neuron 2014 Jul 16;83(2):388-403

PMID:25033182

Rapid in vivo forward genetic approach for identifying axon death genes in Drosophila

Neukomm LJ, Burdett TC, Gonzalez MA, Züchner S and Freeman MR

Proc Natl Acad Sci USA 2014 Jul 8;111(27):9965-9970

PMID:24958874

Signaling mechanisms regulating Wallerian degeneration

Freeman MR

Curr Opin Neurobiol. 2014 Aug;27:224-231

PMID:24907513

Diverse cellular and molecular modes of axon degeneration

Neukomm LJ and Freeman MR

Trends Cell Biol. 2014 Sep;24(9):515-523

PMID:24780172

Drosophila models of neuronal injury

Rooney TM and Freeman MR

ILAR J. 2014;54(3):291-295

PMID:24615442

Glial wingless/Wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction

Kerr KS, Fuentes-Medel Y, Brewer C, Barria R, Ashley J, Abruzzi KC, Sheehan A, Tasdemir-Yilmaz OE, Freeman MR* and Budnik V* (*corresponding/senior authors)

J Neurosci. 2014 Feb 19;34(8):2910-2920

PMID:24553932

Astrocytes engage unique molecular programs to engulf pruned neuronal debris from distinct subsets of neurons

Tasdemir-Yilmaz OE and Freeman MR

Genes Dev. 2014 Jan 1;28(1):20-33

PMID:24361692

2013

Architects in neural circuit design: Glia control neuron numbers and connectivity

Corty MM and Freeman MR

J Cell Biol. 2013 Nov 11;203(3):395-405

PMID:24217617

Evolving concepts in gliogenesis: A look way back and ahead to the next 25 years

Freeman MR and Rowitch DH

Neuron 2013 Oct 30;80(3):613-623

PMID:24183014

Deletions within its subcellular targeting domain enhance the axon protective capacity of Nmnat2 in vivo

Milde S, Fox AN, Freeman MR and Coleman MP

Sci Rep. 2013;3:2567

PMID:23995269

Probing the enigma: Unraveling glial cell biology in invertebrates

Coutinho-Budd J and Freeman MR

Curr Opin Neurobiol. 2013 Dec;23(6):1073-1079

PMID:23896311

The c-Jun kinase signaling cascade promotes glial engulfment activity through activation of draper and phagocytic function

MacDonald JM, Doherty J, Hackett R and Freeman MR

Cell Death Differ. 2013 Sep;20(9):1140-1148

PMID:23618811

2012

Whole genome sequencing and a new bioinformatics platform allow for rapid gene identification in D. melanogaster EMS screens 

Gonzalez MA, Van Booven D, Hulme W, Ulloa RH, Lebrigio RFA, Osterloh J, Logan M, Freeman M and Züchner S

Biology (Basel) 2012 Dec 5;1(3):766-777

PMID:24832518

Integration of a retrograde signal during synapse formation by glia-secreted TGF-ß ligand

Fuentes-Medel Y, Ashley J, Barria R, Maloney R, Freeman MR* and Budnik V* (*corresponding/senior authors)

Curr Biol. 2012 Oct 9;22(19):1831-1838

PMID:22959350

Combining comparative proteomics and molecular genetics uncovers regulators of synaptic and axonal stability and degeneration in vivo

Wishart TM, Rooney TM, Lamont DJ, Wright AK, Morton AJ, Jackson M, Freeman MR and Gillingwater TH

PLoS Genet. 2012;8(8):e1002936

PMID:22952455

Distinct molecular pathways mediate glial activation and engulfment of axonal debris after axotomy

Ziegenfuss JS, Doherty J and Freeman MR

Nat Neurosci. 2012 Jun 17;15(7):979-987

PMID:22706267

dSarm/Sarm1 is required for activation of an injury-induced axon death pathway

Osterloh JM, Yang J, Rooney TM, Fox AN, Adalbert R, Powell EH, Sheehan AE, Avery MA, Hackett R, Logan MA, MacDonald JM, Ziegenfuss JS, Milde S, Hou YJ, Nathan C, Ding A, Brown RH, Conforti L, Coleman M, Tessier-Lavigne M, Züchner S and Freeman MR

Science 2012 Jul 27;337(6093):481-484

PMID:22678360

Negative regulation of glial engulfment activity by Draper terminates glial responses to axon injury

Logan, MA, Hackett R, Doherty J, Sheehan A, Speese SD and Freeman MR

Nat Neurosci. 2012 Mar 18;15(5):722-730

PMID:22426252

WldS protects axons through increased mitochondrial flux and enhanced mitochondrial Ca²⁺ buffering

Avery MA, Rooney TM, Pandya JD, Wishart TM, Gillingwater TH, Geddes JW, Sullivan PG and Freeman MR

Curr Biol. 2012 Apr 10;22(7):596-600

PMID:22425157

Analysis of glial cell development and function in Drosophila

Stork T, Bernardos R and Freeman MR

Cold Spring Harb Protoc. 2012 Jan 1;2012(1):1-17

PMID:22194269

2010

Specification and morphogenesis of astrocytes

Freeman MR

Science 2010 Nov 5;330(6005):774-778

PMID:21051628

Activation of autophagy during cell death requires the engulfment receptor Draper

McPhee CK, Logan MA, Freeman MR and Baehrecke EH

Nature 2010 Jun 24;465(7301):1093-1096

PMID:20577216

Wallerian degeneration, Wld^s, and Nmnat

Coleman MP and Freeman MR

Annu Rev Neurosci. 2010;33:245-267

PMID:20345246

2009

Neuronal death and dismemberment mediated by Sox14

Osterloh JM and Freeman MR

Nat Neurosci. 2009 Dec;12(12):1479-1480

PMID:19935724

Glia and muscle sculpt neuromuscular arbors by engulfing destablilized synaptic boutons and shed presynaptic debris

Fuentes-Medel Y, Logan MA, Ashley J, Ataman B, Budnik V* and Freeman MR* (*corresponding/senior authors)

PLoS Biol. 2009 Aug;7(8):e1000184

PMID:19707574

Ensheathing glia function as phagocytes in the adult Drosophila brain

Doherty J, Logan MA, Tasdemir O and Freeman MR

J Neurosci. 2009 Apr 15;29(15):4768-4781

PMID:19369546

Wld^s requires Nmnat1 enzymatic activity and N16-VCP interactions to suppress Wallerian degeneration

Avery MA, Tasdemir O, Sheehan A, Wang J and Freeman MR

J Cell Biol. 2009 Feb 23;184(4):501-513

PMID:19237597

2008

Draper-dependent glial phagocytic activity is mediated by Src and Syk family kinase signalling

Ziegenfuss JS, Biswas R, Avery MA, Hong K, Sheehan AE, Yeung YG, Stanley ER and Freeman MR

Nature 2008 Jun 12;453(7197):935-939

PMID:18432193

2007

A look inside

Freeman MR

Neuron Glia Biol. 2007 Feb;3(1):1-3

PMID:18634573

The scoop on the fly brain: Glial engulfment functions in Drosophila

Logan MA and Freeman MR

Neuron Glia Biol. 2007 Feb;3(1):63-74

PMID:18172512

Glia got rhythm

Emery P and Freeman MR

Neuron 2007 Aug 2;55(3):337-339

PMID:17678846

2006

The Drosophila cell corpse engulfment receptor Draper mediates glial clearance of severed axons

MacDonald JM, Beach MG, Popiglia E, Sheehan, AE, Watts RJ and Freeman MR

Neuron 2006 Jun 15;50(6):869-881

PMID:16772169

Glial cell biology in Drosophila and mammals

Freeman MR and Doherty J

Trends Neurosci. 2006 Feb;29(2):82-90

PMID:16377000

Sculpting the nervous system: Glial control of neuronal development

Freeman MR

Curr Opin Neurobiol. 2006 Feb;16(1):119-125

PMID:16387489

2005

Glial (and neuronal) cells missing

Freeman MR

Neuron 2005 Oct 20;48(2):163-165

PMID:16242394

Glial control of synaptogenesis

Freeman MR

Cell 2005 Feb 11;120(3):292-293

PMID:15707887

2003

Unwrapping glial biology: Gcm target genes regulating glial development, diversification, and function

Freeman MR, Delrow J, Kim J, Johnson E and Doe CQ

Neuron 2003 May 22;38(4):567-580

PMID:12765609

2001

Moving muscle: Jeb signaling in Drosophila

Freeman MR and Doe CQ

Dev Cell. 2001 Nov;1(5):587-588

PMID:11709176

Asymmetric Prospero localization is required to generate mixed neuronal/glial lineages in the Drosophila CNS

Freeman MR and Doe CQ

Development 2001 Oct;128(20):4103-4112

PMID:11641232

1999

The dare gene: Steroid hormone production, olfactory behavior, and neural degeneration in Drosophila

Freeman MR, Dobritsa A, Gaines P, Segraves WA and Carlson JR

Development 1999 Oct;126(20):4591-4602

PMID:10498693

Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila

Buszczak M, Freeman MR, Carlson JR, Bender M, Cooley L and Segraves WA

Development 1999 Oct;126(20):4581-4589

PMID:10498692

A novel family of divergent seven-transmembrane proteins: Candidate odorant receptors in Drosophila

Clyne PJ, Warr CG, Freeman MR, Lessing D, Kim J and Carlson JR

Neuron 1999 Feb;22(2):327-338

PMID:10069338