Massively Parallel Sequencing Shared Resource (MPSSR)
The OHSU Massively Parallel Sequencing Shared Resource functions as a full service genomics facility serving research scientists and clinicians with a full range of applications based upon massively parallel sequencing protocols.
The OHSU Massively Parallel Sequencing Shared Resource (MPSSR) was founded in 2009 to provide high quality, cost efficient short read sequencing services to help investigators examine the genetic profiles that underlie disease predisposition and to explore the fundamental operations of biological systems. In addition to sequencing services, the MPSSR offers a variety of library construction services - including genome resequencing with sequence capture and transcriptome analysis. Contact us about your proposed work to see how we can assist you.
We offer the following library preparation services: Illumina TruSeq standard and stranded RNA-seq, low input RNA-seq via Nugen Ovation and Clontech SMART-seq, single cell RNA-seq with the 10x Chromium system, miRNA libraries using the Clontech small RNA library kit, and DNA exome capture with the Illumina Nextera RapidCapture protocol.
Funding for our initial Illumina GAIIx sequencer was provided by a generous grant from the M.J. Murdock Charitable Trust. Funding for our upgrade to the HiSeq 2000 was provided by OHSU. Funding for the HiSeq 2500 and the NextSeq 500 was provided by the M.J. Murdock Charitable Trust.
The MPSSR receives support from the Knight Cancer Institute and the Portland Alcohol Research Center (PARC).
Quick Links and Quick Information
- Initiate projects via our new online form at the OHSU iLab portal
- Illumina HiSeq 2500 Sequencer
- Illumina NextSeq 500 Sequencer
- Illumina cBot
- 10x Genomics Chromium Single Cell RNA-seq System
- Covaris S220 sonicator.
- Dedicated blade server for data collection and preparation
- LifeTechnology StepOne Real Time PCR System
- LifeTechnology StepOnePlus Real Time PCR System