Flow Cytometry

OHSU offers a full flow cytometry service to aid in the evaluation of a variety of clinical problems such as:
  • Lymphoma/leukemia
  • Immunodeficiency and Autoimmune disorders
  • Lymphocyte activation for bone marrow transplant patients
  • Paroxysmal nocturnal hemoglobinuria (PNH), hereditary platelet disorders (Bernard-Soulier Syndrome and Glansmann's thrombasthenia)
  • Fetal maternal blood screening test

Flow Lab test menus:

Hematologic malignancies

  • Myeloid disorders
              Acute myeloid leukemias
              Chronic myeloproliferative neoplasms
              Chronic myeloproliferative/myelodysplastic neoplasms
  • Lymphoid disorders
              Acute lymphoblastic leukemia/lymphomas
              B-cells, T-cell, and NK-cell lymphomas, all subtypes
              Large granulocytic leukemia with Kir antibodies
              Plasma cell myelomas
              Sezary syndrome

MRD (minimal residual diseases) detection (sensitivity of 0.01%)

Acute lymphoblastic leukemia
Acute myeloid leukemia
Chronic lymphocytic leukemia

Immunodeficiency and autoimmune disorders

CVID (common variable immunodeficiency)
SCID (severe combined immunodeficiency)
ALPS (autoimmune lymphoproliferative disorders)
CD4 cell count
T-cell subset count: CD3/CD4/CD8
Lymphoid subset count: T, B, and NK cells

Stem cells and bone marrow transplant services:

CD34 count
CD34 count with T-cell quant
Immune reconstitution
Sorting for Chimerism

PNH: High sensitivity assay (0.01%)

Fetal hemoglobin testing

Platelet surface markers for hereditary platelet disorders

Flow sorting

Chimerism testing
Sorting cells on slides for FISH
Sorting cells for RNA expression
Sorting cells for DNA mutation testing

The lab is open for routine testing 8am-5pm weekdays and 9am-4pm on weekends and holidays for OKT3 testing only.

Faculty members can be reached by calling 503 494-2302 during regular business hours or by calling the campus operator after hours at 503 494-8311 and asking for the Immunology faculty on-call.