Selected publications

Analysis of K-Ras Interactions by Biotin Ligase Tagging.
Ritchie C, Mack A, Harper L, Alfadhli A, Stork PJS, Nan X, Barklis E.
Cancer Genomics Proteomics. 2017 Jul-Aug;14(4):225-239.
PMID: 28647697

Analysis of quinolinequinone reactivity, cytotoxicity, and anti-HIV-1 properties.
Alfadhli A, Mack A, Harper L, Berk S, Ritchie C, Barklis E.
Bioorg Med Chem. 2016 Nov 1;24(21):5618-5625. doi: 10.1016/j.bmc.2016.09.028. Epub 2016 Sep 12.
PMID: 27663546

Trimer Enhancement Mutation Effects on HIV-1 Matrix Protein Binding Activities.
Alfadhli A, Mack A, Ritchie C, Cylinder I, Harper L, Tedbury PR, Freed EO, Barklis E.
J Virol. 2016 May 27;90(12):5657-64. doi: 10.1128/JVI.00509-16. Print 2016 Jun 15.
PMID: 27030269 

Analysis of HIV-1 Gag protein interactions via biotin ligase tagging.
Ritchie C, Cylinder I, Platt EJ, Barklis E.
J Virol. 2015 Apr;89(7):3988-4001. doi: 10.1128/JVI.03584-14. Epub 2015 Jan 28.
PMID: 25631074 

RRE-dependent HIV-1 Env RNA effects on Gag protein expression, assembly and release.
López CS, Sloan R, Cylinder I, Kozak SL, Kabat D, Barklis E.
Virology. 2014 Aug;462-463:126-34. doi: 10.1016/j.virol.2014.05.019. Epub 2014 Jun 25.
PMID: 24971705 

The roles of lipids and nucleic acids in HIV-1 assembly.
Alfadhli A, Barklis E.
Front Microbiol. 2014 May 28;5:253. doi: 10.3389/fmicb.2014.00253. eCollection 2014. Review.
PMID: 24917853

Second site reversion of a mutation near the amino terminus of the HIV-1 capsid protein.
López CS, Tsagli SM, Sloan R, Eccles J, Barklis E.
Virology. 2013 Dec;447(1-2):95-103. doi: 10.1016/j.virol.2013.08.023. Epub 2013 Sep 21.
PMID: 24210103 

Analysis of small molecule ligands targeting the HIV-1 matrix protein-RNA binding site.
Alfadhli A, McNett H, Eccles J, Tsagli S, Noviello C, Sloan R, López CS, Peyton DH, Barklis E.
J Biol Chem. 2013 Jan 4;288(1):666-76. doi: 10.1074/jbc.M112.399865. Epub 2012 Nov 7.
PMID: 23135280 

HIV-1 matrix protein binding to RNA.
Alfadhli A, McNett H, Tsagli S, Bächinger HP, Peyton DH, Barklis E.
J Mol Biol. 2011 Jul 22;410(4):653-66. doi: 10.1016/j.jmb.2011.04.063.
PMID: 21762806 

Determinants of the HIV-1 core assembly pathway.
López CS, Eccles JD, Still A, Sloan RE, Barklis RL, Tsagli SM, Barklis E.
Virology. 2011 Aug 15;417(1):137-46. doi: 10.1016/j.virol.2011.05.011. Epub 2011 Jun 14.
PMID: 21676426

Second-site compensatory mutations of HIV-1 capsid mutations.
Noviello CM, López CS, Kukull B, McNett H, Still A, Eccles J, Sloan R, Barklis E.
J Virol. 2011 May;85(10):4730-8. doi: 10.1128/JVI.00099-11. Epub 2011 Mar 2.
PMID: 21367891

Analysis of the N-terminal region of the murine leukemia virus nucleocapsid protein.
Still A, Huseby D, Barklis E.
Virus Res. 2011 Jan;155(1):181-8. doi: 10.1016/j.virusres.2010.10.001. Epub 2010 Oct 8.
PMID: 20934471 

Small molecule inhibition of HIV-1-induced MHC-I down-regulation identifies a temporally regulated switch in Nef action.
Dikeakos JD, Atkins KM, Thomas L, Emert-Sedlak L, Byeon IJ, Jung J, Ahn J, Wortman MD, Kukull B, Saito M, Koizumi H, Williamson DM, Hiyoshi M, Barklis E, Takiguchi M, Suzu S, Gronenborn AM, Smithgall TE, Thomas G.
Mol Biol Cell. 2010 Oct 1;21(19):3279-92. doi: 10.1091/mbc.E10-05-0470. Epub 2010 Aug 11.
PMID: 20702582 

Structure-specific recognition protein 1 facilitates microtubule growth and bundling required for mitosis.
Zeng SX, Li Y, Jin Y, Zhang Q, Keller DM, McQuaw CM, Barklis E, Stone S, Hoatlin M, Zhao Y, Lu H.
Mol Cell Biol. 2010 Feb;30(4):935-47. doi: 10.1128/MCB.01379-09. Epub 2009 Dec 7.
PMID: 19995907 

Analysis of human immunodeficiency virus type 1 matrix binding to membranes and nucleic acids.
Alfadhli A, Still A, Barklis E.
J Virol. 2009 Dec;83(23):12196-203. doi: 10.1128/JVI.01197-09. Epub 2009 Sep 23.
PMID: 19776118 

Characterization of the in vitro HIV-1 capsid assembly pathway.
Barklis E, Alfadhli A, McQuaw C, Yalamuri S, Still A, Barklis RL, Kukull B, López CS.
J Mol Biol. 2009 Mar 27;387(2):376-89. doi: 10.1016/j.jmb.2009.01.058. Epub 2009 Feb 3.
PMID: 19356593 

HIV-1 matrix organizes as a hexamer of trimers on membranes containing phosphatidylinositol-(4,5)-bisphosphate.
Alfadhli A, Barklis RL, Barklis E.
Virology. 2009 May 10;387(2):466-72. doi: 10.1016/j.virol.2009.02.048. Epub 2009 Mar 27.
PMID: 19327811

HIV-1 Nef assembles a Src family kinase-ZAP-70/Syk-PI3K cascade to downregulate cell-surface MHC-I.
Hung CH, Thomas L, Ruby CE, Atkins KM, Morris NP, Knight ZA, Scholz I, Barklis E, Weinberg AD, Shokat KM, Thomas G.
Cell Host Microbe. 2007 Apr 19;1(2):121-33.
PMID: 18005690

Analysis of human immunodeficiency virus matrix domain replacements. 
Scholz, I., Still, A., Dhenub, T., Coday, K., Webb, M., and Barklis, E. (2008). 
Virology 371, 322-335.

The HIV-1 matrix protein assembles on membranes as a hexamer. 
Alfadhli, A., Huseby, D., Kapit, E., Colman, D., and Barklis, E.
(2007). J. Virol. 81, 1472-1478.

Sultam thiourea inhibition of West Nile virus. 
Barklis, E., Still, A., Sabri, M., Hirsch, A., Nikolich-Zugich, J., Brien, J., Dhenub, T., Scholz, I., and Alfadhli, A.
(2007). Antimicrobial Agents and Chemotherapy 51, 2642-2645.

Analysis of human immunodeficiency virus type-1 Gag dimerization-induced assembly. 
Alfadhli, A., Dhenub, T., Still, A., and Barklis, E.
(2005). J. Virol. 79, 14498-14506.

Assembly of human immunodeficiency virus precursor Gag proteins. 
Huseby, D., Barklis, R., Alfadhli, A., and Barklis, E.
(2005). J. Biol. Chem. 280, 17664-17670.

Virus particle core defects caused by mutations in the human immunodeficiency virus capsid N-terminal domain.
Scholz, I., Arvidson, B., Huseby, D., and Barklis, E.
(2005). J. Virol. 79, 1470-1479.