DNA Services Core
The DNA Services Core is a unit of the Integrated Genomics Laboratory and is located on OHSU Marquam Hill Campus in Richard Jones Hall, room 5330. We work closely with the Massively Parallel Sequencing Shared Resource and the Gene Profiling Shared Resource to provide support and services, from specimen processing through data generation.
Please acknowledge support from the core in publications and presentations. See suggested text below.
Services we offer
- Human cell line authentication: Accurate authentication of human cell lines using STR analysis and meeting the ANSI/ATCC requirements
- Oligo synthesis: Creation of custom primers and probes for oligo and siRNA synthesis through Integrated DNA Technologies (IDT)
- DNA quantification: Quantification, size and quality assessment of DNA samples
- DNA normalization and plating: Plating support for sample dilution and standardization for downstream assays
- Consulting: For project design and sample preparation assistance
Acknowledgements and co-authorship:
When presenting and publishing results from projects processed by the DNA Services Core, inclusion of the following statement is appreciated:
"STR profiling for human cell line authentication was performed in the OHSU DNA Services Core; this work utilized a 3730xl DNA Analyzer purchased with funding from NIH SIG grant S10 OD010609"
- Trevor McFarland is leaving the Integrated Genomics Laboratory as of October 1st, 2019. Since 2014, Trevor has applied his knowledge and experience to growing the DNA Services Core and providing excellent support to OHSU researchers. He will continue to advance scientific research at OHSU in Dr. Julie Saugstad’s laboratory in the Department of Anesthesiology and Preoperative Medicine.
- Please contact Britt Daughtry or Chris Harrington for information or questions about core services.
Summer 2019 IGL newsletter
- For updates on service center activities, please see the Summer 2019 IGL newsletter.
Single cell analysis
- To learn more about single cell analysis and available core resources, please visit the University Shared Resources program website.
Sanger sequencing services have moved to Vollum Sequencing Core
- As of July 1st 2018, the DNA Services Core will no longer be supporting Sanger sequencing. Ongoing services utilizing capillary electrophoresis for DNA sequencing will be available through the Vollum Sequencing Core. The DNA Services Core will continue to provide cell line authentication service and support for oligo synthesis.
Assays and technologies
We currently offer a human cell line authentication (CLA) service that uses Promega's GenePrint® 10 system to confirm the identity of human cell lines through short tandem repeat (STR) analysis and to detect the presence of cell line contaminants.
- This service provides co-amplification and detection of 9 STR loci, including Amelogenin (gender determining locus), and can amplify DNA with inputs of 10ng.
- Standards for Cell Line Authentication and Beyond - Almeida et al. (2016)
- Cell line authentication flyer
- DNA oligo synthesis: There is an easy-to-use web ordering portal through Integrated DNA Technologies (IDT) that enables customers to create custom primers and probes for oligo and siRNA synthesis with excellent prices. With this portal you will be able to:
- Eliminate purchase orders and be invoiced directly through iLab
- Conduct cost-effective experiments with better pricing and free standard shipping
- Begin projects sooner with next-day delivery on standard desalted oligos <60 bases
- Nanodrop 1000 and 8000 Spectrophotometers
- ThermoFisher Scientific Veriti Thermal Cyclers
- Eppendorf epMotion 5075 Liquid Handling Robot for sample and reagent aliquoting, and sample normalization and plating
- Suite of custom and commercial software for data QA/QC and analysis
- Access to ABI DNA Analyzer