COVID-19 MODIFIED OPERATIONS

OHSU declared modified operations as of March 23rd, 2020, to help slow the spread of COVID-19 (coronavirus disease 2019).  The core lab was temporarily shut down but received approval to re-open under OHSU Return-to-Research Level 2 modified operations on May 18. The core laboratory has been reorganized to comply with 6 ft distancing requirements, and enhanced PPE and cleaning procedures. 

Research support activities were restarted on June 1st.  Please continue to submit iLab service requests, however, be aware that at this time turnaround times may be slower. Please contact Chris Harrington for questions or more information. Core staff continue to be available by email, phone, and online meeting to plan experiments, advise on sample collection and preparation, review data, and assist with grant development and manuscript preparation.

DNA Services Core

The DNA Services Core is a unit of the Integrated Genomics Laboratory and is located on OHSU Marquam Hill Campus in Richard Jones Hall, room 5330. We work closely with the Massively Parallel Sequencing Shared Resource and the Gene Profiling Shared Resource to provide support and services, from specimen processing through data generation.

Please acknowledge support from the core in publications and presentations. See suggested text below.

Core information flyer

COVID-19 update: IDT is maintaining all oligo services at this time. You may continue to place custom DNA and RNA oligo orders through the DNA Services portal available through iLab.

Services we offer

  • Human cell line authentication: Accurate authentication of human cell lines using STR analysis and meeting the ANSI/ATCC requirements
  • Oligo synthesis: Creation of custom primers and probes for oligo and siRNA synthesis through Integrated DNA Technologies (IDT)
  • DNA quantification: Quantification, size and quality assessment of DNA samples
  • DNA normalization and plating: Plating support for sample dilution and standardization for downstream assays
  • Consulting: For project design and sample preparation assistance

Request services

To request services through the DNA Services Core, go to the iLab website.  For consultations, please contact Britt Daughtry.

If you have any questions or need further information about the services we offer, please contact Britt Daughtry or Chris Harrington.

Acknowledgements and co-authorship:

When presenting and publishing results from projects processed by the DNA Services Core, inclusion of the following statement is appreciated:

"STR profiling for human cell line authentication was performed in the OHSU DNA Services Core; this work utilized a 3730xl DNA Analyzer purchased with funding from NIH SIG grant S10 OD010609"

Major equipment

  • Nanodrop 1000 and 8000 Spectrophotometers
  • ThermoFisher Scientific Veriti Thermal Cyclers
  • Eppendorf epMotion 5075 Liquid Handling Robot for sample and reagent aliquoting, and sample normalization and plating
  • Suite of custom and commercial software for data QA/QC and analysis
  • Access to ABI DNA Analyzer

Announcements

Postponed: UCSC Genome Browser Workshop

  • This event is postponed as of March 11th, 2020.  This event will be updated once a new workshop date has been determined.

Winter 2019 IGL newsletter

Single cell analysis

Sanger sequencing services have moved to Vollum Sequencing Core

  • As of July 1st 2018, the DNA Services Core will no longer be supporting Sanger sequencing. Ongoing services utilizing capillary electrophoresis for DNA sequencing will be available through the Vollum Sequencing Core. The DNA Services Core will continue to provide cell line authentication service and support for oligo synthesis.

Assays and technologies

  • DNA oligo synthesis: There is an easy-to-use web ordering portal through Integrated DNA Technologies (IDT) that enables customers to create custom primers and probes for oligo and siRNA synthesis with excellent prices. With this portal you will be able to:
    • Eliminate purchase orders and be invoiced directly through iLab 
    • Orders must be placed through iLab in order to receive discounted pricing.
    • Conduct cost-effective experiments with better pricing and free standard shipping
    • Begin projects sooner with next-day delivery on standard desalted oligos <60 bases

Frequently viewed

  • Cell line validation service
  • For Massively Parallel Sequencing requests, please go to the homepage of our sister Core facility, MPSSR.
  • For assistance with DNA/RNA isolations and other molecular profiling assays, please visit the GPSR homepage