Molecular Microbiology Lab
Culture, Superficial Wound (Aerobic)
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Lab Section:
Turnaround Time:
72 Hours
Test Schedule:
7 days a week, 7:00 am to 7:00 pm
Specimen Requirements:
Specimen collected under sterile conditions.
Preferred specimen type:
E-Swab (Aerobes and Anaerobes) (Opens in a new window)
Stuart Swab (Aerobes only) (Opens in a new window)
Storage and Transport:
Once a swab sample is collected, it should be placed immediately into the E-Swab transport tube or Stuart Swab, where it comes in contact with the transport medium. It should be transported to the laboratory, preferably within 2 hours of collection to maintain optimum organism viability. If immediate delivery is delayed, then specimens should be refrigerated at 4 to 8 degrees C or stored at room temperature (20 to 25 degrees C) and processed within 48 hours, except for Neisseria gonorrhoeae cultures which should be processed within 24 hours.
Pediatric Specimen Requirements:
As above.
Culture, Lower Respiratory
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Lab Section:
Turnaround Time:
48 Hours
Test Schedule:
7 days a week, 7 am to 7 pm
Specimen Requirements:
Sputum, Endotracheal tube aspirates and Auger suction specimens.
Specimens should be labeled with the sample type, date and time of collection, and at least two patient identifiers before submission to the laboratory.
Stability: 2 hours at room temperature, 24 hours at 2 to 8 Celsius.
Pediatric Specimen Requirements:
Neonates, infants and small children cannot provide an expectorated sputum sample, so the sample should be collected from the upper airway using an Auger. Frequently, a very small amount of sputum secretions can be obtained by this method, so the sample may be submitted in the catheter in a sterile container.
Culture, Strep A
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Includes:
Strep. pyogenes (Group A beta Strep), Group C beta Strep, Group G beta Strep, Arcanobacterium haemolyticum.
Turnaround Time:
48 hours.
Test Schedule:
7 days a week.
Specimen Requirements:
Collect E-swab. Stability (from collection to initiation): Ambient for 24 hours.
Unacceptable Conditions: Frozen specimen, nonsterile or leaking containers.
Comments:
Reported: Preliminary in 1 day; Final verified in 2 days.
Synonyms:
Culture, Throat
Strep A Culture
Throat
Throat Culture
Hepatitis B by PCR
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Lab Section:
Turnaround Time:
7 to 10 days.
Test Schedule:
Tuesdays and Fridays.
Units:
Specimen Requirements:
6 mL of blood in purple (EDTA) tube. Spin within 24 hours and freeze 2.5 mL plasma immediately.
Alternatively, serum from a red top tube may be prepared, frozen, and submitted for the assay.
Pediatric Specimen Requirements:
1.0 mL plasma, processed as above.
Synonyms:
HBV
Hep B
Hep B Quant
Hepatitis B Quant
Epstein-Barr Virus (EBV) by PCR, Quantitative
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Lab Section:
Turnaround Time:
3 days.
Test Schedule:
Monday through Friday.
Specimen Requirements:
5 to 10 mL blood in a LAVENDER top tube. Send to lab within 6 hours OR spin and freeze plasma immediately (1 mL minimum). Transport frozen.
Synonyms:
Epstein-Barr Virus Quant, Plasma
EBV Viral Load
HPV by PCR with Genotyping
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Lab Section:
Turnaround Time:
1 to 3 days.
Test Schedule:
Tuesdays and Thursdays.
Specimen Requirements:
Cervical specimens collected in BD SurePath™ Preservative Fluid can be transported at 2 to 30 degrees C.
Comments:
This PCR test simultaneously detects all 14 high risk carcinogenic HPV subtypes. The HPV genotypes included are 16, 18, and a pool of twelve others (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68). SurePath has not been approved by the FDA for HPV testing using the Cobas system, but has been validated by the OHSU Lab for in vitro diagnostic testing. OHSU Lab is a CLIA and CAP accredited laboratory for high complexity clinical testing.
Synonyms:
High-Risk HPV
HPV
Human Papillomavirus High-Risk DNA Test
COVID-19, PCR
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Lab Section:
Turnaround Time:
48 hours.
Test Schedule:
Monday through Sunday.
Critical Values:
Detected and inconclusive results are critical values.
Specimen Requirements:
Sample type: nasopharyngeal (NP) swab, oropharyngeal (OP) swab, bronchoalveolar lavage (BAL), nasal aspirates, nasal washes, endotracheal aspirates, and saliva.
Sample volume: 1 to 3 mL VTM, UTM or PBS. Minimal sample is 500 microliters.
Storage conditions: at room temperature (15 to 30 °C) for up to 8 hours and refrigerated (2 to 8 °C) up to 3 days.
Comments:
Specificity is 98% and sensitivity is 96% (330 viral copies/ml).
Synonyms:
SARS-CoV-2
HIV-1 Viral Load, Quantitative
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Lab Section:
Turnaround Time:
2 to 5 days.
Test Schedule:
Tuesdays and Thursdays.
Specimen Requirements:
5 to 10 mL blood in LAVENDER top tube. Spin within 24 hours and freeze plasma (2.5 mL) immediately. Specimens collected in green-top tubes (heparin) are unsuitable for testing.
Synonyms:
HIV-1 RNA
HIV-1 Viral Load Ultra Quantitative
Hepatitis C Quantitative
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Lab Section:
Turnaround Time:
3 days.
Test Schedule:
Tuesdays and Fridays.
Specimen Requirements:
6 mL blood in a LAVENDER top (EDTA) tube. Spin within 24 hours and freeze 2.5 mL plasma immediately.
Alternatively, serum from a red top tube may be separated, frozen, and submitted for testing.
Synonyms:
HCV
HCV Viral Load
Hep C
Hep C Viral Load, Hep C
Hepatitis C Viral Load
HQT
CMV Quantitative, Plasma
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Lab Section:
Turnaround Time:
1 to 3 days.
Test Schedule:
Test performed daily, Monday through Friday.
Specimen Requirements:
5 to 10 mL blood in a LAVENDER top tube. Send to lab within 6 hours or spin and freeze plasma (1 mL minimum) immediately. Transport frozen.
Pediatric Specimen Requirements:
2.5 mL blood minimum; 1 mL plasma
Synonyms:
CMV Quant.
Quantitative CMV DNA PCR
Cytomegalovirus DNA Quant