Orderable EAP code:

LAB103090

Billable EAP Codes:

80002035

CPT Codes:

86708

Lab Section:

Immunology

Turnaround Time:

Routine: 2 days 

Test Schedule:

7 days a week 

Interpretation:

Nonreactive = Hepatitis A IgG antibodies not detected

Reactive = Hepatitis A IgG antibodies detected

Specimen Requirements:

2 ml blood collected in serum separator tube (SST). Send 0.5 mL serum aliquot.

Minimum volume = 250 uL

Stability:

Room Temperature (21 - 30°C) – 16 hours

Refrigerated (2-8 C) - 8 days

Specimen may be stored on or off the clot, or separator gel

Frozen -20°C or colder – 30 days

Remove specimen from clot or separator gel prior to storage

Pediatric Specimen Requirements:

2 ml blood collected in serum separator tube (SST). Send 0.5 mL serum aliquot.

Minimum volume = 250 uL

Stability:

Room Temperature (21 - 30°C) – 16 hours

Refrigerated (2-8 C) - 8 days

Specimen may be stored on or off the clot, or separator gel

Frozen -20°C or colder – 30 days

Remove specimen from clot or separator gel prior to storage

Reference Range:

Nonreactive 

Comments:

Use this test to determine the immune status of individuals to Hepatitis A virus (HAV) infection.

This test is not intended for use in screening blood, plasma or tissue donors.

Orderable EAP code:

LAB103975

Billable EAP Codes:

80001899 x 1

CPT Codes:

86592 x 1

Lab Section:

Immunology

Includes:

RPR Qualitative

If reactive, reflex to:

RPR Quant (Titer)

Additional charges will apply

Turnaround Time:

2 Days

Test Schedule:

7 days a week

Specimen Requirements:

4.0 mL blood in serum separator tube (SST) or nonanticoagulated tube (RED top).

Remove 2.0 mL serum from clot tube and send in an aliquot tube.

Minimum volume = 1 mL serum

Storage/Transport Temperature: Refrigerated

Stability:

Refrigerated (2-8 C) - 5 days

If delay in testing, store frozen at -20 C or colder.

Avoid repeated freeze/thaw cycles.

Remove specimen from clot or separator gel prior to storage.

Pediatric Specimen Requirements:

4.0 mL blood in serum separator tube (SST) or nonanticoagulated tube (RED top).

Remove 2.0 mL serum from clot tube and send in an aliquot tube.

Minimum volume = 1 mL serum

Storage/Transport Temperature: Refrigerated

Stability:

Refrigerated (2-8 C) - 5 days

If delay in testing, store frozen at -20 C or colder.

Avoid repeated freeze/thaw cycles.

Remove specimen from clot or separator gel prior to storage.

Reference Range:

RPR Qual: Nonreactive

RPR Quant (Titer): Less than 1:1

Comments:

Preferred test for monitoring treatment response in established syphilis patients. No TP-PA will be reflexed.

Orderable EAP code:

LAB102550

Billable EAP Codes:

80002037 x 1

CPT Codes:

87340 x 1

Lab Section:

Immunology

Includes:

Hepatitis B Surface Antigen If Reactive reflex to:

Hepatitis B Surface Antigen, Confirmation (LAB102548)

Additional charges apply.

Turnaround Time:

Routine: 2 days, additional time for reflex testing.

Test Schedule:

7 days a week.

Interpretation:

Nonreactive = Specimen is considered negative for HBsAg.

Pending Confirmation = Screening test is reactive and pending confirmation, as hepatitis B surface antigen screening test may yield a false positive result.

Specimen Requirements:

Minimum testing age: >28 days. 

4 ml blood collected in serum separator tube (SST) or EDTA tube. Send 2 mL serum or plasma aliquot.

Minimum volume = 250 uL, 1.5 mL is needed to include reflex testing.

Stability:

Room Temperature (15 - 30°C) – 24 hours

Refrigerated (2-8 C) - 6 days

Specimen may be stored on or off the clot, or separator gel.

Frozen -10°C or colder – 3 freeze/thaw cycles

Remove specimen from clot or separator gel prior to storage.

Pediatric Specimen Requirements:

Minimum testing age: >28 days. 

4 ml blood collected in serum separator tube (SST) or EDTA tube. Send 2 mL serum or plasma aliquot. 

Minimum volume = 250 uL, 1.5 mL is needed to include reflex testing.

Stability:

Room Temperature (15 - 30°C) – 24 hours

Refrigerated (2-8 C) - 6 days

Specimen may be stored on or off the clot, or separator gel.

Frozen -10°C or colder – 3 freeze/thaw cycles

Remove specimen from clot or separator gel prior to storage.

Reference Range:

Nonreactive

Comments:

Used for the qualitative detection of hepatitis B surface antigen (HBsAg).  May be used to screen for HBV infection in pregnant women to identify neonates who are at risk for acquiring hepatitis B during the perinatal period.  May be used to provide presumptive evidence of infection with the hepatitis B virus (HBV).

This test is not intended for use in screening blood, plasma or tissue donors.

Hepatitis B Surface Antigen, Confirmation is sent to AHPL for testing.

Synonyms:

HBS

HBSAG

Hep B Surf AG

Hep B Surf AG Confirmation

Orderable EAP code:

LAB103756

Billable EAP Codes:

80002963 x 1

CPT Codes:

86780 x 1

Lab Section:

Immunology

Includes:

Treponema Pallidum Antibody. If reactive, reflex to RPR Qualitative.

• Reactive reflex goes to RPR Quant (Titer)

• Nonreactive reflex = T.PALLIDUM AB By TP-PA

Additional charges will apply.

Turnaround Time:

Routine: 2 days, additional time for reflex testing. 

Test Schedule:

7 days a week

Interpretation:

Result	Final Interpretation
Non - Reactive	Non-reactive for treponema antibodies
Reactive	Reactive for treponema antibodies

Related Links:

Syphilis Test Algorithm

Specimen Requirements:

4.0 mL blood in serum separator tube (SST). Send 2 mL serum aliquot.

Minimum volume is 1.5 mL serum.

Storage/Transport Temperature: Refrigerated. Remove specimen from clot or separator gel prior to storage.

Stabile refrigerated (2 to 8 C) for 5 days. Stable frozen -20 C or colder for 8 to 30 days.

Pediatric Specimen Requirements:

*Do not order for patients less than 18 months. See comments below.*

4.0 mL blood in serum separator tube (SST). Send 2 mL serum aliquot.

Minimum volume is 1.5 mL serum.

Storage/Transport Temperature: Refrigerated. Remove specimen from clot or separator gel prior to storage.

Stabile refrigerated (2 to 8 C) for 5 days. Stable frozen -20 C or colder for 8 to 30 days.

Reference Range:

Treponema pallidum Antibodies: Nonreactive

RPR: Nonreactive

RPR Titer: Less than 1:1

Comments:

Recommended test for syphilis screen for patients 18 months old or older (reverse algorithm).

For patients younger than 18 months, refer to RPR With Reflex to Titer and TP-PA Confirmation (LAB00282)

T.PALLIDUM AB By TP-PA is sent to ARUP for testing. Treponema pallidum Antibody by TP-PA (Opens in a new window).

Orderable EAP code:

LAB100629

Billable EAP Codes:

80002066 x 1 80002067 x 1 80002068 x 1

CPT Codes:

82784 x 3

Lab Section:

Immunology

Includes:

IgA, Serum

IgG, Serum

IgM, Serum

Turnaround Time:

Routine: 4 days

Test Schedule:

Monday, Wednesday, and Friday.

Specimen Requirements:

2 mL blood in a GOLD top tube. Separate serum from cells ASAP or within 2 hours of collection. Send 1 mL aliquot refrigerated. 

Stability: 

Plasma is stable for 7 days refrigerated at 2-8 C

Pediatric Specimen Requirements:

Minimum volume = 0.5mL serum

Reference Range:

IgG 768 - 1632 (mg/dL)

IgA 68 - 408 (mg/dL)

IgM 35 – 263 (mg/dL)

Synonyms:

IgA

IgG

IgM

Orderable EAP code:

LAB103801

Billable EAP Codes:

80002037 x 1 80002034 x 1 80001526 x 1 80002039 x 1 80002388 x 1 (if reflexed) 80005297 x 1 (if reflexed)

CPT Codes:

87340 x 1 86803 x 1 86705 x 1 86709 x 1 87522 x 1 (if reflexed) 87341 x 1 (if reflexed)

Lab Section:

Immunology

Includes:

HEPATITIS B SURFACE AG W/REFLEX CONFIRMATION [LAB102550]

HEPATITIS C VIRUS W/CONFIRMATION [LAB00105]

HEPATITIS B CORE AB, IGM, SERUM [LAB00513]

HEPATITIS A AB IGM, SERUM [LAB00110]

LAB103801 can also reflex to:

HEPATITIS C QUANTITATIVE [LAB00776]

HEPATITIS B SURFACE ANTIGEN, CONFIRMATION [LAB102548]

Turnaround Time:

See individual test listing

Test Schedule:

See individual test listing

Interpretation:

See individual test listing

Specimen Requirements:

See individual test listing

Pediatric Specimen Requirements:

See individual test listing

Reference Range:

See individual test listing

Synonyms:

Acute Hep Panel, reflex to confirmation

HAV IgM Ab

HBsAg confirmation

HBV core IgM Ab; HCV Ab

Hep Panel reflex to confirmation

Hepatitis A Antibody, IgM

Hepatitis B Core Antibody, IgM

Hepatitis B Surface Antigen

Hepatitis C Antibody

Hepatitis C Antibody by CIA

Hepatitis panel

Hepatitis screening panel

Hepatitis Screening Profile, Serum

Hepatitis, Acute Panel

Viral hepatitis panel

Orderable EAP code:

LAB103499

Billable EAP Codes:

80002113 x 1

CPT Codes:

87389 x 1

Lab Section:

Immunology

Includes:

HIV AB/AG Screen. If Screen is Reactive, reflex to:

HIV-1 RNA Quant (LAB00779)

HIV Ab Confirmation 

Turnaround Time:

Routine: 2 days, additional time for reflex testing. 

Test Schedule:

HIV AB/AG Screen: 7 days a week

HIV RNA Quant: Tuesday and Friday

HIV AB Confirm: 7 days a week

Specimen Requirements:

6 mL LAVENDER top (EDTA) vacutainer. Purple top only for reflex to viral load testing. Send 1 mL aliquot of plasma refrigerated, do not aliquot all plasma off the cells. Send plasma and cells.

Stability:

Whole blood collected in EDTA tubes may be stored and/or transported for up to 24 hours at room temp or refrigerated (2 to 25 C) prior to plasma separation. 

After plasma separation, refrigerated (2 to 8 C) plasma is stable for up to 7 days for HIV AB/AG Screen and HIV AB Confirm, 6 days for HIV RNA QUANT.

Pediatric Specimen Requirements:

Do not order for pediatric patients less than 2 years of age, see Comment section below.

Minimum volume for testing is 1 mL plasma, do not aliquot all plasma off the cells. Send plasma and cells.

Reference Range:

Nonreactive

Comments:

Billable EAP and CPT Codes: Reflexes create additional charges. 

Do not order for pediatric patients less than 2 years of age. NIH guidelines recommend virologic assays (i.e. RNA tests) that directly detect HIV for diagnosis of HIV infection in infants younger than 2 years.

This test is not intended for use in screening blood, plasma or tissue donors.

Synonyms:

AIDS Antibody Screen

Acquired Immunodeficiency Syndrome

HIV Antibody Screen

HIV - 1,2 AB/ HIV-1 P24 AG Screen

HIV P-24 Antigen

Human Immunodeficiency Virus

P-24 Antigen

Orderable EAP code:

LAB103478

Billable EAP Codes:

80002025 x 1 80002029 x 1 86334 x 1 (SPEP Profee) 84165 x 1 (Immunotyping Profee) 80002029 x 1 (if both ISUB and IFE)

CPT Codes:

84165 x 1 86334 x 1 86334 x 1 (SPEP Profee) 84165 x 1 (Immunotyping Profee) 86334 x 1 (if both ISUB and IFE)

Lab Section:

Immunology

Includes:

Protein Electrophoresis

Immunotyping

Reflex to Immunofixation 

Turnaround Time:

Routine: 7 days

Test Schedule:

Monday through Friday.

Units:

g/dL

Specimen Requirements:

2 mL blood in a GOLD top tube. Separate serum from cells ASAP or within 2 hours of collection.

Send 1 mL aliquot refrigerated.

Stability: 7 days refrigerated 2-8°C

Pediatric Specimen Requirements:

Minimum volume: 0.5 mL serum

Reference Range:

Albumin: 3.5 - 5.2 g/dL

Alpha-1: 0.2 - 0.5 g/dL

Alpha-2: 0.5 -1.1 g/dL

Beta: 0.5 - 1.1 g/dL

Gamma: 0.6 - 1.5 g/dL

Comments:

Protein electrophoresis and immunotyping (immunosubtraction) are performed by capillary electrophoresis. Confirmation of monoclonal proteins performed by immunofixation gel electrophoreses if needed. 

Synonyms:

IFE

Immunoelectrophoresis

Immunofixation

Monoclonal Protein Detection Quantitation and Characterization

MGUS study

Monoclonal Protein Study

Multiple Myeloma Study

Protein ELP

Protein Electrophoresis

Serum Protein Electrophoresis

SPEP

Orderable EAP code:

LAB103340

Billable EAP Codes:

80002066 x 1 80002067 x 1 80002068 x 1 80005520 x 2 80002025 x 1 80002029 x 1 86334 x 1 (SPEP Profee) 84165 x 1 (Immunotyping Profee) 80002029 x 1 (if both ISUB and IFE)

CPT Codes:

82784 x 3 83521 x 2 84165 x 1 86334 x 1 86334 x 1 (SPEP Profee) 84165 x 1 (Immunotyping Profee) 86334 x 1 (if both ISUB and IFE)

Lab Section:

Immunology

Turnaround Time:

Routine: 6 days

Test Schedule:

SPEP reflex to Immunotyping: Monday through Friday.

Immunoglobulin Panel: Monday, Wednesday, and Friday.

Specimen Requirements:

Labels for two specimens will print.



SPEP reflex to Immunotyping: 4 mL blood in a GOLD top tube. Separate serum from cells ASAP or within 2 hours of collection. A minimum of 2.0 mL serum is needed.

Immunoglobulin Panel Serum: 2 mL blood in a GOLD top tube. Separate serum from cells ASAP or within 2 hours of collection. A minimum of 1.0 mL serum is needed.

Orderable EAP code:

LAB00282

Billable EAP Codes:

80001899 x 1

CPT Codes:

86592 x 1

Lab Section:

Immunology

Includes:

RPR Qualitative. If reactive, reflex to:

• RPR Quant (Titer)

• T. PALLIDUM AB By TP-PA

Additional charges will apply.

Turnaround Time:

Routine: 2 days, additional for reflex testing. 

Test Schedule:

7 days a week

Related Links:

Syphilis Test Algorithm

Specimen Requirements:

*Do not order for patients 18 months or older. See comments below.*

4.0 mL blood in serum separator tube (SST) or nonanticoagulated tube (RED top). 

Remove 2.0 mL serum from clot tube and send in an aliquot tube.

Minimum volume is 1 mL serum

Storage/Transport Temperature: Refrigerated

Stable refrigerated (2 to 8 C) for 5 days. If there is a delay in testing, store frozen at -20 C or colder. Avoid repeated freeze/thaw cycle. Remove specimen from clot or separator gel prior to storage.

Pediatric Specimen Requirements:

4.0 mL blood in serum separator tube (SST) or nonanticoagulated tube (RED top).

Remove 2.0 mL serum from clot tube and send in an aliquot tube.

Minimum volume is 1 mL serum

Storage/Transport Temperature: Refrigerated

Stable refrigerated (2 to 8 C) for 5 days. If there is a delay in testing, store frozen at -20 C or colder. Avoid repeated freeze/thaw cycle. Remove specimen from clot or separator gel prior to storage.

Comments:

Recommended test for congenital syphilis screen for patients less than 18 months old (traditional algorithm).

For patients 18 months or greater refer to Syphilis Antibody Screen W/ Reflex To RPR, Titer, and TP-PA Confirm (LAB103756).

T.PALLIDUM AB By TP-PA is sent to ARUP for testing Treponema pallidum Antibody by TP-PA T Treponema Pallidum Antibody by TP-PA (Opens in a new window)

Synonyms:

Syphilis Serology

Syphilis Reverse Testing Algorithm

Rapid Plasma Reagin

Rapid Plasma Reagin Reflex to Titer or TPPA