DNA Services Core: Sequencing, AFLP and Oligo Synthesis

welcome

We are located in RJH 5330, around the corner from the Synapse & Java Junction. (Three "after hours" drop-off refrigerators for weekend and evening workaholics 8-)

We perform long-read (Sanger) DNA sequencing and fragment length analysis, aka genotyping. And we negotiated good prices from outside service providers for custom oligonucleotides.

We  consult on sequence assembly and other data processing, informatics, and database needs.

DNA sequencing & fragment analysis are accomplished with our state-of-the-art, Applied Biosystems 3730xl, 48 capillary fluorescence instrument. 

Several levels of service are available to match your needs and finances. Submit an on-line work-request.

We provide oligo design assistance and trouble-shooting of applications using oligonucleotides. We act as a store front for several synthesis sources. By pooling our orders we can get the best prices and services. Use the on-line workrequest for small orders. Batch orders of small scale oligo synthesis can be as low as 10¢ per base. Discounts on other products too. Check the Fees page for the new prices.

DNA Services Core Informational Flyer

The Template Quality Challenge

As the saying goes, "GIGO". The reverse is also true, that is, the cleaner the template, the cleaner and longer the read.

The CE run giving the highest quality read, based on parameters indicating good template quality (signal strength, signal to noise, clear length read (Q > 20), etc.) will get a voucher for $54 of free sequencing! 

[Fineprint: You must purify the template yourself to qualify. And for the pros out there: You can only win once a year.]

And The Winner IS ...

• Robyn Eustis (Bartlett Lab in Biology at PSU) for "Best plasmid template" with a contiguous read length of 1109 with 942 high quality (>Q20) bases. Congrats Robyn!
  

Last month we sequenced ~2500 templates, including fosmids, BACs or large plasmids, PCR products of various sizes and more than 1970 'normal' plasmids. Of the latter, ~80% produced contiguous read lengths of 800 bases or more.

Holiday Schedule

We are closed Thursday (Thanksgiving Day) and Friday (Stay home and watch football day) the 28th and 29th.

 

Please give us samples early (before 10 AM on Wednesday the 27th) to get your data that evening.

Disclaimer

As we attempt to break even as a University Shared Resource, we may need to change our fees so revenues are approximately equal to expenses. Check About/Fees for our current rates.