Educational Links and Publications

Proteomics Overview

An overview of mass spectrometry based proteomics. Includes most of the revelant information from the links found below. Written by John Klimek for first time users of PSR. Mass_Spectrometry_Based_Proteomics

This tutorial publication from the journal Proteomics works its way through several different types of mass analyzers, and describes how a mass spectrometer measures the mass/charge ratio of peptides.

Your Co-IP Experiment

This document goes over some of the sample preparation concerns unique to the Co-IP experiment. It also summarizes PSR's procedures for processing the samples, gives pointers for assessing final data from the experiment, and lists some approximate costs. Co-IP Guide.

Scaffold Viewer Users Guide

Now updated for version 3.0; an unofficial guide for installing and using the free scaffold viewer for mass spec results. Scaffold Viewer

PAW Pipeline Users Guide

The PAW Pipeline was developed by Phil Wilmarth of OHSU as an improvement over previous open source protein identification tools; the initial publication can be found here. This software package provides PSR with customizable state-of-the-art protein identification software, and has significantly increased sensitivity in larger and more complex datasets. 

PAW Results Guide: an overview of the results spreadsheets generated by PAW and help with basic data interpretation. 
PAW User's Guide: an overview on how to run Comet and the PAW Pipeline.

Educational Publications

Proteomics: a pragmatic perspective is a recent article from Nature Biotechnology. It is a guide intended for those new to Proteomics, and is helpful for setting realistic expectations for your Proteomics experiment. It discusses issues such as how much protein you need to do an experiment, how many proteins you can reasonably expect to find, and rough ideas of how complicated it will be to get certain types of results.

Challenges for proteomics core facilities is an overview of the difficulties many labs like PSR struggle with on a day-to-day basis. It covers topics like quality control, data archiving, and funding.

OHSU researchers develop new model to study S. mutans behavior at protein level is an example of what is possible for us to do under the University Shared Resources pilot funds program.


PSR Publications

The following is a selection of recent publications resulting from PSR Projects.

Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but
is Dispensable for Hair-Bundle Development and Function

Stereocilia-staircase spacing is influenced bymyosin III motors and their cargos
espin-1 andespin-like

PDZD7-MYO7A complex identified in enriched stereocilia membranes

Plastin 1 widens stereocilia by transforming actin filament packing from
hexagonal to liquid

Extended multiplexing of TMT labeling reveals age and high fat diet specific
proteome changes in mouse epididymal adipose tissue.

Increased Hemodynamic Load in Early Embryonic Stages Alters Endocardial to
Mesenchymal Transition

Evidence that the Rhodopsin Kinase (GRK1) N‑Terminus and theTransducin
GαC‑Terminus Interact with the Same"HydrophobicPatch"on Rhodopsin TM5

y-Crystallins of the chicken lens: remnants of an ancientvertebrate gene family
in birds

Identifying Family-Member-Specific Targets ofMono-ARTDs by Using a Chemical
Genetics Approach

Data detailing the platelet acetyl-lysine proteome

Separating myoblast differentiation from muscle cell fusion using IGF-I and the
p38 MAP kinase inhibitor SB202190