Matthew Brush


Research Assistant Professor


  • Ontology development, data modeling, and curation for Monarch Initiative, eagle-I, and CTSA Connect projects
  • Development of best-practices and documentation for community ontology engineering and data curation
  • Active development, coordination, and integration of broader community ontology efforts


Ph.D. in Molecular Cancer Biology, Duke University, 2000-2005
B.S. in Biology, University of Notre Dame, 1996-2000

Research and Work experience

  • Ontologist and Data Curator, Oregon Health and Science University Ontology Development Group (2010-present)
  • National Library of Medicine Post-Doctoral Fellow in Bioinformatics, Oregon Health Sciences University (2009-2012), Advisor: Dr. Melissa Haendel
  • Post-Doctoral Fellow, Oregon Health Sciences University Vollum Institute for Advanced Biomedical Research (2008-2009), Advisor : Dr. Gary Thomas
  • Post-Doctoral Fellow, Duke University Medical Center Department of Pharmacology and Cancer Biology (2005-2007). Advisors Dr. Timothy Haystead and Dr. Shirish Shenolikar
  • Graduate Student, Duke University Medical Center Department of Pharmacology and Cancer Biology (2000-2005), Advisor : Dr. Shirish Shenolikar
  • Undergraduate Research Assistant, University of Notre Dame Department of Biological Sciences, Advisor: Dr. Martin Tenniswood
  • Summer Research Intern, Pepperdine University Natural Sciences Division (1999), Advisor: Dr. Donna Nofziger-Plank
  • Summer Research Intern, University of California at San Francisco Department of Pathology (1998),  Advisor:  Dr. James H. McKerrow

SElect publications and presentations


  • Vasilevsky NA, Brush MH, Paddock H, Ponting L, Tripathy SJ, LaRocca GM, Haendel MA. (2013) On the reproducibility of science: unique identification of research resources in the biomedical literature. PeerJ 1:e148
  • Nicole Vasilevsky, Tenille Johnson, Karen Corday, Carlo Torniai, Matthew Brush, Erik Segerdell, Melanie Wilson, Chris Shaffer, David Robinson and Melissa Haendel Research Resources: Curating the new eagle-i discovery system, Database, Volume 2012, 2012, doi: 10.1093/database/bar067
  • Zhou W*, Brush MH*, Choy MS, Shenolikar S., Association with endoplasmic reticulum promotes proteasomal degradation of GADD34 protein.  J Biol Chem. 2011 Jun 17;286(24):21687-96. Epub 2011 Apr 25. (* co-first author)
  • Aslan JE,  You H, Williamson DM, Endig J, Shu H, Du Y, Milewski RL, Youker RT,  Thomas L, Brush MH, Possemato A, Sprott K, Fu H, Greis KD, Runckel DN, Vogel A and Thomas G.  Akt and 14-3-3 control a PACS-2 homeostatic switch that integrates membrane traffic with Apo2L/TRAIL-induced apoptosis. Mol Cell. 2009 May 14;34(4):497-509
  • Brush MH, Shenolikar S. Control of cellular GADD34 levels by the 26S proteasome.  (2008) Mol Cell Biol. Dec;28(23):6989-7000.
  • Voltz JW, Brush M, Sikes S, Steplock D, Weinman EJ, Shenolikar S. Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets. (2007) J Biol Chem. Nov 16;282(46):33879-87.
  • Hagerty L, Weitzel DH, Chambers J, Fortner CN, Brush MH, Loiselle D, Hosoya H, Haystead TA. ROCK1 phosphorylates and activates zipper-interacting protein kinase (2007) J Biol Chem.. Feb 16;282(7):4884-93.
  • Roadcap DW, Brush MH, and Shenolikar S. Identification of Protein Phosphatase-1 Regulators. (2006) Methods in Molecular Biology 365:181-96.
  • Brush MH, Guardiola A, Connor JH , Yao TP, and Shenolikar S. Deactylase inhibitors disrupt cellular complexes containing protein phosphatases and deacetylases. (2004)  J Biol Chem. 279:7685-91.
  • Brush MH, Weiser DC, and Shenolikar S. GADD34 Targets Protein Phosphatase-1 to the endoplasmic reticulum to facilitate the dephosphorylation of the eukaryotic initiation factor, eIF2α (2003) Mol Cell Biol. 23:1292-303.
  • Caffrey CR, Schanz M, Nkemgu-Njinkeng J, Brush M, Hansell E, Cohen FE, Flaherty TM, McKerrow JH, Steverding D. Screening of acyl hydrazide proteinase inhibitors for antiparasitic activity against Trypanosoma brucei. (2002) Int J Antimicrob Agents. Mar;19(3): 227-31.


  • Brush MH, Development and Application of ReO: A Biomedical Research Reagent Ontology.  National Library of Medicine Training Conference, Madison, WI. 2012  (abstract author and presented plenary session talk)
  • Brush MH, Vasilevsky N, Torniai C, Haendel M. The Reagent Ontology: an Integrated Resource for Curation of Biomedical Research Reagents. ISB Biocuration Conference,  Washington D.C., USA. 2012 (poster presentation)
  • Torniai C, Brush M, Vasilevsky N, Segerdell E, Wilson M, Johnson T, Corday K, Shaffer C, Haendel M. Developing an Application Ontology for Biomedical Resource Annotation and Retrieval: Challenges and Lessons Learned, International Conference on Biomedical Ontology Conference Preceedings, Buffalo, NY, USA. 2011 (abstract author)
  • Brush MH, Vasilevsky N, Torniai C, Johnson T, Shaffer C, Haendel MA. Developing a Reagent Application Ontology within the OBO Foundry Framework, International Conference on Biomedical Ontology Conference Preceedings, Buffalo, NY, USA. 2011 (poster presentation)
  • S. Shenolikar and M.H. Brush, Recovery from Cell Stress: Novel Paradigms for Regulating Protein Phosphatase-1, EMBO Workshop on Protein Phosphatases, Aviero, Portugal. 2007 (abstract author)
  • Brush MH and Shenolikar S, A GADD34-Protein Phosphatase-1 Complex Promotes Translational Recovery From Cellular Stress, Lorne Conference on Protein Structure and Function. Lorne, Australia.  2006  (poster presentation)
  • Brush MH and Shenolikar S, GADD34 Turnover Restores Translational Control Through eIF2α Phosphorylation Following Cellular Stress, American Society for Cell Biology Annual Meeting. Washington D.C., USA. 2004  (poster presentation)
  • Brush MH and Shenolikar S, GADD34-Mediated eIF2α dephosphorylation Promotes Tranalsional Recovery Following Cell Stress, FASEB Conference on Protein Phosphatases. Snowmass CO, USA. 2002  (poster presentation)