OHSU

Michiko Nakano

Michiko Nakano

Email: 
Phone:  503 748-4078
Fax:  503 748-1464

 

Current Appointments

Research Professor, Division of Environmental and Biomolecular Systems

 

Education

Ph. D., Cell Biology, University of Tokyo, 1976

 

Biography

  • Mitsubishi-Kasei Institute of Life Science, Postdoctoral Fellow 1977-78
  • Meiji College of Pharmacy, Tokyo, Assistant Professor, 1978-82
    Associate Professor, 1982-87
  • Oklahoma State University, Research Associate, 1987-88
  • Louisiana State University Medical Center at Shreveport, Research Associate, 1988-1995
    Research Assistant Professor, 1995-1998
  • OGI School of Science and Engineering, Research Assistant Professor, 1998-2000

 

Research Interests

Anaerobiosis of Bacillus subtilis; oxygen-controlled gene regulation; two-component signal transduction system; transcriptional activation; nitrate/nitrite reductases; flavohemoglobin; anaerobic electron transport; nitric oxide signaling.

 

Selected Publications

Kommineni, S.; Lama, A.; Popescu, B.; Nakano, M. M. (2012) Global transcriptional control by NsrR in Bacillus subtilis. J. Bacteriol., 194, 1679-1688. (DOI: 10.1128/jb.06486-11)

Zuber, P.; Chauhan, S.; Pilaka, P.; Nakano, M. M.; Gurumoorthy, S.; Lin, A. A.; Barendt, S. M.; Chi, B. K.; Antelmann, H.; Maeder, U. (2011) Phenotype enhancement screen of a regulatory spx mutant unveils a role for the ytpQ gene in the control of iron homeostasis. PLoS One, 6, e25066. (DOI: 10.1371/journal.pone.0025066)

Yukl, E. T.; Ioanoviciu, A.; Sivaramakrishnan, S.; Nakano, M. M.; Ortiz, d. M. P. R.; Moenne-Loccoz, P. (2011) Nitric oxide dioxygenation reaction in DevS and the initial response to nitric oxide in Mycobacterium tuberculosis. Biochemistry, 50, 1023-1028. (DOI: 10.1021/bi1015315)

Siluvai, G. S.; Nakano, M.; Mayfield, M.; Blackburn, N. J. (2011) The essential role of the Cu(II) state of Sco in the maturation of the CuA center of cytochrome oxidase: evidence from H135Met and H135SeM variants of the Bacillus subtilis Sco. J. Biol. Inorg. Chem., 16, 285-297. (DOI: 10.1007/s00775-010-0725-z)

Serve, O.; Kamiya, Y.; Maeno, A.; Nakano, M.; Murakami, C.; Sasakawa, H.; Yamaguchi, Y.; Harada, T.; Kurimoto, E.; Yagi-Utsumi, M.; Iguchi, T.; Inaba, K.; Kikuchi, J.; Asami, O.; Kajino, T.; Oka, T.; Nakasako, M.; Kato, K. (2010) Redox-dependent domain rearrangement of protein disulfide isomerase coupled with exposure of its substrate-binding hydrophobic surface. J. Mol. Biol., 396, 361-374. (DOI: 10.1016/j.jmb.2009.11.049)

Nakano, M. M.; Lin, A.; Zuber, C. S.; Newberry, K. J.; Brennan, R. G.; Zuber, P. (2010) Promoter recognition by a complex of Spx and the C-terminal domain of the RNA polymerase α subunit. PLoS One, 5. (DOI: 10.1371/journal.pone.0008664)

Kommineni, S.; Yukl, E.; Hayashi, T.; Delepine, J.; Geng, H.; Moenne-Loccoz, P.; Nakano, M. M. (2010) Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter. Mol. Microbiol., 78, 1280-1293. (DOI: 10.1111/j.1365-2958.2010.07407.x)

Siluvai, G. S.; Nakano, M. M.; Mayfield, M.; Nilges, M. J.; Blackburn, N. J. (2009) H135A controls the redox activity of the sco copper center: Kinetic and spectroscopic studies of the His135Ala variant of Bacillus subtilis. Sco. Biochemistry, 48, 12133-12144. (DOI: 10.1021/bi901480g)

Huang, T.; Geng, H.; Miyyapuram, V. R.; Sit, C. S.; Vederas, J. C.; Nakano, M. M. (2009) Isolation of a variant of subtilosin A with hemolytic activity. J. Bacteriol., 191, 5690-5696. (DOI: 10.1128/jb.00541-09)

Yukl, E. T.; Ioanoviciu, A.; Nakano, M. M.; Ortiz, d. M. P. R.; Moenne-Loccoz, P. (2008) A distal tyrosine residue is required for ligand discrimination in DevS from Mycobacterium tuberculosis. Biochemistry, 47, 12532-12539. (DOI: 10.1021/bi801234w)

Yukl, E. T.; Elbaz, M. A.; Nakano, M. M.; Moenne-Loccoz, P. (2008) Transcription factor NsrR from Bacillus subtilis senses nitric oxide with a 4Fe-4S cluster. Biochemistry, 47, 13084-13092. (DOI: 10.1021/bi801342x)