Fluorescence Spectroscopy

TECAN Spark Multi-mode plate reader

Multi-mode plate reader
The TECAN Spark Multi-mode plate reader.

The core provides access to a TECAN Spark Multi-mode plate reader and a SpectraMax M Series multi-Mode microplate reader. The TECAN Spark Multi-mode plate reader is capable of

  • Absorbance – incl. UV/VIS spectra
  • Fluorescence top & bottom - incl. spectra
  • Time resolved fluorescence (TRF) – incl. spectra
  • FRET
  • TR-FRET
  • Fluorescence Polarization (FP)
  • Luminescence – glow, flash, multicolor, spectra
  • Automatic injections

Cost for using the TECAN plate reader

Training: This process charge is for training personnel on instrument usage and theory. ($125.00/hr)

Consultations and Data Collection/Analysis: This charge is for training personnel, helping to collect, analyze and interpret data. ($125.00/hr)

Experiment hourly use: This is the hourly rate for the instrument. A minimum time of booking is 15 min ($40.00/hr).

CARY Eclipse fluorescence spectrophotometer

Fluorescence spectrophotometer
The CARY Eclipse fluorescence spectrophotometer equipped with a temperature controller and a RX 2000 stopped flow attachment

 In addition the Core has a CARY Eclipse fluorescence spectrophotometer equipped with capacity for temperature and time spectrum acquisition in 4 cuvettes at multiple wavelengths. The Cary fluorimeter provides the user with superior sensitive data collection, with enhanced sampling capabilities and excellent reliability. Together these instruments offer users:

  • A unique uncompromised combination of filters to provide sensitivity and a monochromator for wavelength flexibility,
  • The ability to get rapid absorbance readouts with a high speed monochromator.
  • The option of precise temperature control during assay development by using a unique thermoelectric cooling module.
  • High sensitivity, uniformity and linearity for assays with unique temperature sensing and correction, plus pre-set filter settings for specific experiments such as measuring the thermodynamics and kinetics of binding, analyzing enzyme activity kinetics, ELISA, Cell-based fluorescence studies and thermo-stability of biologics
  • The ability to obtain rapid kinetics using a temperature controlled, with a pneumatic driven RX2000 stopped flow attachment with a dead-time of only 8 ms.  The flow cells has dual path lengths of 2 and 10 mm, with per-shot volume of 120 ml.

Cost for using the CARY Eclipse instrument

Training: This process charge is for training personnel on instrument usage and theory. ($125.00/hr)

Consultations and Data Collection/Analysis: This charge is for training personnel, helping to collect, analyze and interpret data. ($125.00/hr)

Experiment hourly use: This is the hourly rate for the instrument. A minimum time of booking is 15 min ($40.00/hr).

RX 2000 stopped-flow setup and usage: This charge is configuring the stopped-flow apparatus. ($50.00 setup and $20.00/hr).