OHSU

TEM

FEI Titan Krios™ Cryo-Transmission Electron Microscope

FEI Living Lab Titan Krios™ TEM


Features:

• Robotic sample handling
• Loading of 12 samples
• Parallel illumination
• Optimized for Structural (cellular) Biology applications: cryo tomography, single particle analysis, electron crystallography
• Dual axis stage (+/- 70 degrees) enabling dual axis tomography
• Demonstrated 2.8 angstrom resolution on a virus specimen
• Information Limit: < .14 nm at zero tilt
• STEM capabilities

Titan Krios™ Technical Bulletin


About the Krios

The FEI Titan Krios™ cryo-Transmission Electron Microscope is a state-of-the-art, dedicated imaging robot, optimized for both high-resolution 3D imaging of proteins and macromolecular assemblies, and high-resolution, dual-axis, cellular tomography. Its revolutionary cryo-based technology and stability permits a full range of semi-automated applications, including: 2D electron crystallography, single particle analysis, cryo-electron microscopy, and dual-axis cellular tomography of frozen hydrated samples. Cryo-techniques preserve sample integrity by maintaining the sample in its natural condition and state. 


Location:
Floor Zero, Richard Jones Hall
Online:  September 2012
One of 50 or fewer high-resolution, cryo-electron microscopes in the world.
Requires a specialized environment: low vibration and heat differential tolerance, an absence of electromagnetic currents.
Achieves atomic resolution data.
Good for achieving high-resolution, high-quality optics for cryo samples, as opposed to fixed samples. 
Has low-dose capabilities, achieves balance between showing good information and destroying the sample.

FEI Tecnai™ with iCorr™ (Integrated Light and Transmission Electron Microscope)

FEI's Tecnai with iCorr at OHSU 

FEATURES

 • Locate precise regions of interest quickly
 • Wide field excitation (470-490 nm)
 • Optimized for green fluorescence
 • Objective lens (NA 0.5, 0.65 um res)
 • 5 Mpixel CCD
 • Fluorescence & reflection mode
 • Software controlled


 

 

 

 




 

Tecnai™ with iCorr™ Product Flyer



ABOUT THE FEI Tecnai™ with iCORR™


The most critical "tie point" between imaging modalities is between fluorescence (FM) and electron microscopy (EM), with EM providing Angstrom to nanometer resolution information about molecular ultrastructure and FM providing nanometer to micrometer resolution information about functional molecular complexes.  Scientists from FEI and the Biomolecular Imaging program at Utrecht University have developed novel sample fixation and staining procedures for the Tecnai™ with iCorr™ that preserve both ultrastructural and molecular features for FM and transmission electron microscopy (TEM) and reduce sample preparation times. 

 TEM correlative workflow


Location:
Multnomah Pavilion
Online: November 2012
One of two in the world (the other is located in the Netherlands); this one is the first to be commercially available.
Correlates light microscopy and electron microscopy. Uses light microscopy to find a rare cell or event and uses electron microscopy to study that event.
Ideal for identifying rare cells, events in a large sample, such as 1 in 10,000 events or cells.

CM120 Biotwin

Location: Multnomah Pavilion