Mouse Genetic Models
If you are interested in obtaining one of our currently available mouse models, or a cryopreserved model, please contact Dr. John Crabbe, PARC Animal and Phenotyping Core Director.
Jump to individual tables:
|Line Designation||Description||Developing PI|
|FAST-1, FAST-2, SLOW-1, SLOW-2||FAST replicate 1&2 and SLOW replicate 1&2. Lines were bred to display heightened (FAST) or reduced (SLOW) sensitivity to the locomotor activating effects of a single 2.0 g/kg IP injection of ethanol, compared to response after saline injection; all are embryo cryopreserved and available from the MMRRC.||Tamara Phillips
|WSP-1, WSP-2,WSR-1, WSR-2||Withdrawal Seizure-Prone replicate 1&2 and Withdrawal Seizure-Resistant replicate 1&2. Lines were bred to display severe (WSP) or mild (WSR) handling-induced convulsions after withdrawal from chronic ethanol vapor inhalation. Live mice are available.||John Crabbe
|WSC-1, WSC-2||Withdrawal Seizure Control replicate 1&2.Randomly bred controls bred in parallel with WSP and WSR selected lines. Live mice are available.||John Crabbe
|IWSP-1, IWSP-2, IWSR-1, IWSR-2||Inbred strains derived from WSP & WSR selectively bred lines, beginning with generation S26G33-52. Fully inbred. Live mice are available from IWSP-2 and IWSR-1; for the other two strains, sperm are cryopreserved.||John Crabbe
|HOT-1, HOT-2, COLD-1, COLD-2||HOT & COLD mouse lines were selectively bred for extreme (COLD) or mild (HOT) hypothermia 30-60 minutes after 3 g/kg ethanol. Embryos are cryopreserved.||John Crabbe
|HDID-1, HDID-2||The HDID replicate mouse lines are being actively selectively bred for high blood ethanol concentrations after consumption of ethanol (20%) in a limited access period of 4 hours called, "Drinking in the Dark (DID)."||John Crabbe
|HAFT-1, LAFT-1||The High Acute Functional Tolerance (HAFT) and Low Acute Functional Tolerance (LAFT) replicate 1 mouse lines were selectively bred by Drs. Erwin & Deitrich (UCHSC, Denver, CO) to display high (HAFT) or low (LAFT) acute functional tolerance to ethanol, calculated as the difference in blood ethanol concentrations at the time of regaining the ability to stay for 1 minute on a stationary dowel (12.7mm) after two consecutive injections of ethanol (1.75 g/kg followed by 2.0 g/kg, IP). Embryos are cryopreserved and available from the MMRRC.||John Crabbe
FAST/SLOW, WSP/WSR, HOT/COLD, and HAFT/LAFT selections were bred bi-directionally (high vs low) over 15 or more generations for the trait indicated from the founder populations of the HS/Ibg 8-way inbred strain cross (formerly available from the Institute of Behavior Genetics, Boulder, CO). Independently-derived replicated lines were developed for all four selections. The HAFT/LAFT selection was created by Dr. V. G. Erwin (UCHSC, Denver, CO) and breeding stock of the first replicate were provided to Dr. Crabbe in 2006.
HDID selections are being bred uni-directionally (high lines only) for the trait indicated from founder populations of the HS/Npt 8-way inbred strain cross (available from Dr. R. Hitzemann, OHSU, Portland, OR). These replicated lines are at 22 and 15 generations, respectively (November, 2011).
Short Term Selectively Bred (STSB) lines are periodically developed for various behavioral traits by PARC Researchers for use in gene mapping and microarray studies.