Pilot Project Abstract - Hill, Ann, PhD

Hill, Ann, PhD, Associate Professor, Dept. of Molecular Microbiology and Immunology, OHSU

“The relationship between CMV infection, distorted CD8 T cell compartment and response to influenza vaccination in the elderly”

Elderly persons are more likely to be hospitalized or die from influenza and its related complications than younger adults; however, elderly individuals frequently fail to respond to flu vaccination. The specific goal for the pilot project is (a) to identify biomarkers in the peripheral blood that predict a poor response to influenza vaccine in the elderly, and (b) to determine whether these biomarkers associated with cytomegalovirus (CMV) activity. We will recruit approximately 100 elderly (70+ years of age) health plan members seeking influenza vaccination during the annual Kaiser Permanente flu shot clinics. We will collect blood and urine samples from each participant at the baseline visit and at the follow-up visit four weeks after vaccination. Flu vaccine will be administered during the initial visit by Kaiser Permanente clinical staff as part of routine clinical care. We will measure the correlation between CMV titre and CD8+CD28-population size, CD8+CD28- population size and response to flu vaccine, CMV serostatus and titer and response to flu vaccine, and CMV shedding on the day of vaccination and the response to flu vaccine. This pilot study will allow us to develop both our participant recruitment methods and our laboratory methods, and the preliminary data we collect will support a larger research proposal and a continued collaboration between OHSU and CHR.