Xiaorui Shi, M.D., Ph.D., Associate Professor

Xiaorui Shi, MD, PhD

Email:
Phone: 503 494-2997
Lab phone: 503 494-7149
Office: HRC 0426

 

Background

Xiaorui Shi, M.D., Ph.D., is an Associate Professor with the Oregon Hearing Research Center. Dr. Shi joined OHSU in 2005.

Overview

Current appointments:

  • Associate Professor in the Department of Otolaryngology-Head and Neck Surgery
  • Vice Director of the Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

Previous appointment(s):

  • Associate Professor with the Department of Otolaryngology, the General Hospital of the CPAPA, Beijing, China

 Degrees:

  • M.D. degree from Henan Medical University, Henan, China:
  • M.S. degree from Shanghai Second Medical University, Shanghai, China
  • Ph.D. degree from Henan Medical University, Henan, China

Summary of current research

Research Interests

Normal hearing requires the blood supply to the cochlea is well-regulated and the blood-labyrinth barrier tightly-controlled. The structural integrity of the microvasculature is critical to its function in maintaining the supply of oxygen, nutrients, and hormones to our ears, and for removing toxins and metabolic waste products. Insufficient blood supply to the ear is among the primary causes of age-related and noise-induced hearing loss, and is implicated in autoimmune inner ear disease and several genetically-linked hearing diseases as well. The aim of my lab is to obtain a better understanding of the mechanisms by which blood flow to the ear is regulated, blood-labyrinth barrier is controlled, and compromised vasculature is restored after damage. Ultimately, our goal is to develop new treatments, ameliorate damage, and improve the quality of life for people who suffer from vascular dysfunction related hearing loss. 

  • Regulation of cochlear blood flow
  • Cellular mechanisms controlling the integrity of the blood-labyrinth-barrier
  • Vascular pathology in aging, loud sound, and inflammatory induced damage to the ear
  • Vascular remodeling and angiogenesis after damage

Significant Discoveries and Advances

  • A fibrocyte-pericyte coupling controls regional cochlear blood flow
  • Perivascular resident macrophages in the cochlear blood-labyrinth barrier renew via migration of bone-marrow-derived cells 
  • Pericyte plasticity in response to loud-sound microvessel damage
  • Bone-marrow cells are recruited to the acoustically damaged blood-labyrinth barrier
  • Mass spectroscopy shows a high percentage of proteins in purified mouse strial capillaries are transporters
  • Developed a novel "sandwich-dissociation" method for isolation of strial capillaries and a "mini-chip" method for isolation and primary culture of strial blood-labyrinth-barrier component cells″ Breakdown of the strial BLB, a common feature in a wide range of pathological conditions (cochlear inflammation, loud sound trauma, and ageing), can be remodeled by stem cells 

News

Join Dr. Shi who is the recipient of the 2016 OHSU Technology Transfer & Business Awards, for having your technology, OHSU #2188, Cochlear endothelial cells (ECs), pericytes (PCs) and perivascular resident macrophage-like melanocytes (PVM/Ms) cell line, licensed in fiscal year 2016. The awards ceremony is scheduled for the evening of Wednesday, October 12, in the Vey auditorium of the Doernbecher Children's Hospital building. There will be hors d'oeuvres and drinks served during the opening reception at 4:30 PM and the ceremony will start promptly at 5:30 PM. Please register.

ANI News, Beta June 15, 2016
Going deaf from blaring earphones may soon be history for noise junkies

Public Release: August 29, 2013
EurekAlert! AAAS: Now hear this: Scientists discover compound to prevent noise-related hearing loss

School of Medicine, OHSU: Paper of the Month July 5, 2011
A mystery of the ear solved

FindingHearingAids.com Articles
Scientists Discover Compound to Prevent Hearing Loss

Notable Publication(s)

Said, M.B., Grati, M.h., Ishimoto, T., Zou, B., Chakchouk, I., Ma, Q., Yao, Q. Hammami, B. Yan, D., Mittal, R., Nakamichi, N., Ghorel, A., Neng, L., Tekin, M., Shi, X., Kato, Y., Masmoudi, S., Lu, Z., Hmani, M. and Liu, X. (2016). A mutation in SLC22A4 encoding an organic cation transporter expressed in the cochlea strial endothelium causes human recessive non-syndromic hearing loss DFNB60. Hum Genet 135(5): 513-524. pdf

Selected publications

Shi, X. (2016). Pathophysiology of the cochlear intrastrial fluid-blood barrier (review). Hear Res, doi:10.1016/j.heares.2016.01.010. pdf

Wilson, T., Omelchenko, I., Foster, S., Zhang, Y., Shi, X. & Nuttall, A. L. (2014). JAK2/STAT3 inhibition attenuates noise-induced hearing loss. PLoS One, 9(10), e108276. pdf

Shi, X., Zhang, F., Urdang, Z., Dai, M., Neng, L., Zhang, J., Chen, S., Ramamoorthy, S. & Nuttall, A. L. (2014). Thin and open vessel windows for intra-vital fluorescence imaging of murine cochlear blood flow. Hear Res, 313, 38-46. pdf

Zhang, F., Dai, M., Neng, L., Zhang, J. H., Zhi, Z., Fridberger, A. & Shi, X. (2013). Perivascular macrophage-like melanocyte responsiveness to acoustic trauma—a salient feature of strial barrier associated hearing loss. FASEB J, 27(9), 3730-3740. pdf

Zhang, F., Zhang, J., Neng, L., & Shi, X. (2013). Characterization and inflammatory response of perivascular-resident macrophage-like melanocytes in the vestibular system. J Assoc Res Otolaryngol, 14(5), 635-643. pdf

Neng, L., Zhang, W., Hassan, A., Zemla, M., Kachelmeier, A., Fridberger, A., Auer, M. & Shi, X. (2013). Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear. Nat Prot, 8(4), 709-720. pdf

Zhang, W., Dai, M., Fridberger, A., Hassan, A., DeGagne, J., Neng, L., Zhang, F., He, W., Ren, T., Trune, D. Auer, M. & Shi, X.R. (2012). Perivascular-resident macrophage-like melanocytes in the inner ear are essential for the integrity of the intrastrial fluid–blood barrier. Proc Nat'l Acad Sci U S A, 109(26), 10388-10393. pdf

Dai, M, Yang, Y., Omelchenko, I., Nuttall, A.L., Kachelmeier, A., Xiu, R. & Shi, X.R. (2010). Bone marrow cell recruitment for repair of acoustically damaged blood-labyrinth-barrier mediated by a local iNOS/SDF-1α signal pathway. Am J Path, 177(6):3089-99. PMC2993278. Am J Pathol. 2009 May;174(5):1692-704. pdf

Shi X. (2009) Cochlear pericyte responses to acoustic trauma and the involvement of hypoxia-inducible factor-1alpha and vascular endothelial growth factor. Am J Path, 174(5):1692-1704. pdf

Please see Dr. Shi's full list of publications at PubMed.