Lydie Trautmann, Eng.D., Ph.D.

The identification of mechanisms allowing for an efficient vs. a dysfunctional cellular immune response is critical for the development of new therapeutic strategies against HIV and other infectious agents. The overarching goal of Dr. Trautmann’s research is to provide the scientific knowledge for the development of innovative therapeutic strategies aimed at enhancing the cell-mediated control of viral replication and preventing tissue damage in viral infections. This research focuses mainly on human studies for discovery but the laboratory also participates in preclinical and translational research. Dr. Trautmann focused her work in recent years on the early immunological events occurring in acute HIV infection to identify key mechanisms determining the efficacy or dysfunction of cellular immune responses. Identifying these key mechanisms in the context of natural HIV infection or following vaccination will provide clues for therapeutic strategies to control viral replication, reduce pathogenesis and design effective interventions. This work on T cell immunology is highly synergistic with the research developed at the Vaccine and Gene therapy Institute. 

Three research areas focused on the role of immune cells in prevention and treatment developed in the laboratory are:

  1. Development of effective T cell responses after infection and vaccination
  2. HIV immuno-pathogenesis in lymphoid tissues and CNS on successful ART 
  3. Strategies to induce an immune-mediated HIV remission

Dr. Trautmann’s laboratory uses a variety of unique cell-based assays including human/simian primary T cell culture, expansion and cloning, TCR repertoire sequencing, advanced flow cytometry analysis, and functional assays to measure immune functions that are key in immunological processes: cytolytic activity of cytotoxic T cells, priming of naïve T cells by antigen presenting cells, T cell metabolism, and T cell effector and memory differentiation.

Biography:

Dr. Trautmann received her Engineer degree in 1998 at the École Supérieure de Biotechnologie de Strasbourg, and her Ph.D. in Immunology at Paris V University in 2003. She performed her postdoctoral training at University of Montreal from 2004 to 2009.  She was appointed Assistant then Associate Member at the Vaccine and Gene Therapy Institute of Florida before joining the U.S. Military HIV Research Program in 2015 as Chief of the Cellular Immunology Section. Since September 2019, Dr. Trautmann is Associate Professor at the Vaccine and Gene Therapy Institute.

Selected publications:

Mousseau G, Aneja R, Clementz MA, Mediouni S, Lima NS, Haregot A, Kessing CF, Jablonski JA, Thenin-Houssier S, Nagarsheth N, Trautmann L, Valente ST. Resistance to the Tat Inhibitor Didehydro-Cortistatin A Is Mediated by Heightened Basal HIV-1 Transcription. MBio. 2019 Jul 2;10(4). doi: 10.1128/mBio.01750-18. PubMed PMID: 31266880; PubMed Central PMCID: PMC6606815. 

Takata H, Kessing C, Sy A, Lima N, Sciumbata J, Mori L, Jones RB, Chomont N, Michael NL, Valente S, Trautmann L. Modeling HIV-1 Latency Using Primary CD4+ T Cells from Virally Suppressed HIV-1-Infected Individuals on Antiretroviral Therapy. J Virol. 2019 Jun 1;93(11). doi: 10.1128/JVI.02248-18. Print 2019 Jun 1. PubMed PMID: 30918072; PubMed Central PMCID: PMC6532074. 

Kityo C, Makamdop KN, Rothenberger M, Chipman JG, Hoskuldsson T, Beilman GJ, Grzywacz B, Mugyenyi P, Ssali F, Akondy RS, Anderson J, Schmidt TE, Reimann T, Callisto SP, Schoephoerster J, Schuster J, Muloma P, Ssengendo P, Moysi E, Petrovas C, Lanciotti R, Zhang L, Arévalo MT, Rodriguez B, Ross TM, Trautmann L, Sekaly RP, Lederman MM, Koup RA, Ahmed R, Reilly C, Douek DC, Schacker TW. Lymphoid tissue fibrosis is associated with impaired vaccine responses. J Clin Invest. 2018 Jul 2;128(7):2763-2773. doi: 10.1172/JCI97377. Epub 2018 May 21. PubMed PMID: 29781814; PubMed Central PMCID: PMC6025977.

Colby DJ, Trautmann L, Pinyakorn S, Leyre L, Pagliuzza A, Kroon E, Rolland M, Takata H, Buranapraditkun S, Intasan J, Chomchey N, Muir R, Haddad EK, Tovanabutra S, Ubolyam S, Bolton DL, Fullmer BA, Gorelick RJ, Fox L, Crowell TA, Trichavaroj R, O'Connell R, Chomont N, Kim JH, Michael NL, Robb ML, Phanuphak N, Ananworanich J. Rapid HIV RNA rebound after antiretroviral treatment interruption in persons durably suppressed in Fiebig I acute HIV infection. Nat Med. 2018 Jul;24(7):923-926. doi: 10.1038/s41591-018-0026-6. Epub 2018 Jun 11. PubMed PMID: 29892063; PubMed Central PMCID: PMC6092240. 

Kessing CF, Nixon CC, Li C, Tsai P, Takata H, Mousseau G, Ho PT, Honeycutt JB, Fallahi M, Trautmann L, Garcia JV, Valente ST. In Vivo Suppression of HIV Rebound by Didehydro-Cortistatin A, a "Block-and-Lock" Strategy for HIV-1 Treatment. Cell Rep.2017 Oct 17;21(3):600-611. doi: 10.1016/j.celrep.2017.09.080. PubMed PMID: 29045830; PubMed Central PMCID: PMC5653276. 

Kessing CF, Spudich S, Valcour V, Cartwright P, Chalermchai T, Fletcher JL, Takata H, Nichols C, Josey BJ, Slike B, Krebs SJ, Sailsuta N, Lerdlum S, Jagodzinski L, Tipsuk S, Suttichom D, Rattanamanee S, Zetterberg H, Hellmuth J, Phanuphak N, Robb ML, Michael NL, Ananworanich J, Trautmann L. High Number of Activated CD8+ T Cells Targeting HIV Antigens Are Present in Cerebrospinal Fluid in Acute HIV InfectionJ Acquir Immune Defic Syndr. 2017 May 1;75(1):108-117. doi: 10.1097/QAI.0000000000001301. PubMed PMID: 28177966; PubMed Central PMCID: PMC5388590. 

Takata H, Buranapraditkun S, Kessing C, Fletcher JL, Muir R, Tardif V, Cartwright P, Vandergeeten C, Bakeman W, Nichols CN, Pinyakorn S, Hansasuta P, Kroon E, Chalermchai T, O'Connell R, Kim J, Phanuphak N, Robb ML, Michael NL, Chomont N, Haddad EK, Ananworanich J, Trautmann LDelayed differentiation of potent effector CD8+ T cells reducing viremia and reservoir seeding in acute HIV infection. Sci Transl Med. 2017 Feb 15;9(377). doi: 10.1126/scitranslmed.aag1809. PubMed PMID: 28202771; PubMed Central PMCID: PMC5678930. 

Muir R, Metcalf T, Tardif V, Takata H, Phanuphak N, Kroon E, Colby DJ, Trichavaroj R, Valcour V, Robb ML, Michael NL, Ananworanich J, Trautmann L, Haddad EK. Altered Memory Circulating T Follicular Helper-B Cell Interaction in Early Acute HIV Infection. PLoS Pathog. 2016 Jul;12(7):e1005777. doi: 10.1371/journal.ppat.1005777. eCollection 2016 Jul. PubMed PMID: 27463374; PubMed Central PMCID: PMC4963136. 

Mousseau G, Kessing CF, Fromentin R, Trautmann L, Chomont N, Valente ST. The Tat Inhibitor Didehydro-Cortistatin A Prevents HIV-1 Reactivation from Latency. MBio.2015 Jul 7;6(4):e00465. doi: 10.1128/mBio.00465-15. PubMed PMID: 26152583; PubMed Central PMCID: PMC4495168. 

Muyanja E, Ssemaganda A, Ngauv P, Cubas R, Perrin H, Srinivasan D, Canderan G, Lawson B, Kopycinski J, Graham AS, Rowe DK, Smith MJ, Gaucher D, Isern S, Michael S, Silvestri G, Vanderford TH, Castro E, Pantaleo G, Singer J, Gillmour J, Kiwanuka N, Nanvubya A, Schmidt C, Birungi J, Cox J, Haddad EK, Kaleebu P, Fast P, Sekaly RP, Trautmann L. Immune activation alters cellular and humoral responses to yellow fever 17D vaccine. J Clin Invest. 2014 Jul;124(7):3147-58. doi: 10.1172/JCI75429. Epub 2014 Jun 9. PubMed PMID: 24911151; PubMed Central PMCID: PMC4071376. 

Noto A, Ngauv P, Trautmann L. Cell-based flow cytometry assay to measure cytotoxic activity. J Vis Exp. 2013 Dec 17;(82):e51105. doi: 10.3791/51105. PubMed PMID: 24378436; PubMed Central PMCID: PMC4111270. 

Trautmann L, Mbitikon-Kobo FM, Goulet JP, Peretz Y, Shi Y, Van Grevenynghe J, Procopio FA, Boulassel MR, Routy JP, Chomont N, Haddad EK, Sekaly RP. Profound metabolic, functional, and cytolytic differences characterize HIV-specific CD8 T cells in primary and chronic HIV infection. Blood. 2012 Oct 25;120(17):3466-77. doi: 10.1182/blood-2012-04-422550. Epub 2012 Sep 6. PubMed PMID: 22955926; PubMed

Janbazian L, Price DA, Canderan G, Filali-Mouhim A, Asher TE, Ambrozak DR, Scheinberg P, Boulassel MR, Routy JP, Koup RA, Douek DC, Sekaly RP, Trautmann L. Clonotype and repertoire changes drive the functional improvement of HIV-specific CD8 T cell populations under conditions of limited antigenic stimulationJ Immunol.2012 Feb 1;188(3):1156-67. doi: 10.4049/jimmunol.1102610. Epub 2011 Dec 30. PubMed PMID: 22210916; PubMed Central PMCID: PMC3262882. 

Mbitikon-Kobo FM, Bonneville M, Sekaly RP, Trautmann L. Ex vivo measurement of the cytotoxic capacity of human primary antigen-specific CD8 T cells. J Immunol Methods.2012 Jan 31;375(1-2):252-7. doi: 10.1016/j.jim.2011.09.008. Epub 2011 Oct 5. PubMed PMID: 21996428. 

Said EA, Dupuy FP, Trautmann L, Zhang Y, Shi Y, El-Far M, Hill BJ, Noto A, Ancuta P, Peretz Y, Fonseca SG, Van Grevenynghe J, Boulassel MR, Bruneau J, Shoukry NH, Routy JP, Douek DC, Haddad EK, Sekaly RP. Programmed death-1-induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection. Nat Med. 2010 Apr;16(4):452-9. doi: 10.1038/nm.2106. Epub 2010 Mar 7. PubMed PMID: 20208540; PubMed Central PMCID: PMC4229134. 

Chomont N, El-Far M, Ancuta P, Trautmann L, Procopio FA, Yassine-Diab B, Boucher G, Boulassel MR, Ghattas G, Brenchley JM, Schacker TW, Hill BJ, Douek DC, Routy JP, Haddad EK, Sékaly RP. HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation. Nat Med. 2009 Aug;15(8):893-900. doi: 10.1038/nm.1972. Epub 2009 Jun 21. PubMed PMID: 19543283; PubMed Central PMCID: PMC2859814. 

van Grevenynghe J, Procopio FA, He Z, Chomont N, Riou C, Zhang Y, Gimmig S, Boucher G, Wilkinson P, Shi Y, Yassine-Diab B, Said EA, Trautmann L, El Far M, Balderas RS, Boulassel MR, Routy JP, Haddad EK, Sekaly RP. Transcription factor FOXO3a controls the persistence of memory CD4(+) T cells during HIV infection. Nat Med. 2008 Mar;14(3):266-74. doi: 10.1038/nm1728. Epub 2008 Mar 2. PubMed PMID: 18311149. 

Trautmann L, Janbazian L, Chomont N, Said EA, Gimmig S, Bessette B, Boulassel MR, Delwart E, Sepulveda H, Balderas RS, Routy JP, Haddad EK, Sekaly RP. Upregulation of PD-1 expression on HIV-specific CD8+ T cells leads to reversible immune dysfunction. Nat Med. 2006 Oct;12(10):1198-202. doi: 10.1038/nm1482. Epub 2006 Aug 20. PubMed PMID: 16917489. 

Trautmann L, Rimbert M, Echasserieau K, Saulquin X, Neveu B, Dechanet J, Cerundolo V, Bonneville M. Selection of T cell clones expressing high-affinity public TCRs within Human cytomegalovirus-specific CD8 T cell responses. J Immunol. 2005 Nov 1;175(9):6123-32. doi: 10.4049/jimmunol.175.9.6123. PubMed PMID: 16237109. 

Trautmann L, Labarrière N, Jotereau F, Karanikas V, Gervois N, Connerotte T, Coulie P, Bonneville M. Dominant TCR V alpha usage by virus and tumor-reactive T cells with wide affinity ranges for their specific antigens. Eur J Immunol. 2002 Nov;32(11):3181-90. doi: 10.1002/1521-4141(200211)32:11<3181::AID-IMMU3181>3.0.CO;2-2. PubMed PMID: 12555663. 

Complete list of publications