Structural and functional studies of molecular machines in the ubiquitin system, presented by Minglei Zhao, Assistant Professor, Department of Biochemistry and Molecular Biology, University of Chicago

November 8, 2022
12 p.m. to 1 p.m.
3232 SW Research Drive
Portland, Oregon 97239
Room: M1441 Vollum Auditorium
Contact Information
Samantha Wagner
CPB Seminar: Minglei Zhao


The ubiquitin system plays important roles in many physiological processes in eukaryotes. In this talk, I will present our recent progress on structural and functional characterizations of two important molecular machines involved in the ubiquitin system, p97 and Ubr1. p97 is a type-II AAA ATPase and acts as a central hub to process various ubiquitinated substrates in the ubiquitin system. Human p97 is associated with several neurodegenerative diseases and is also a cancer drug target. Ubr1 is the first genetically cloned E3 ligase, and is involved in the N-degron pathway. We used single-particle cryo-EM as a major tool to determine their structures in complex with the ubiquitinated substrates in multiple states, which provided mechanistic insights into these molecular machines.