Photo of Oleg Varlamov

Oleg Varlamov

  • (503) 346-5377
    • Research Assistant Professor Oregon National Primate Research Center

My research mission is dedicated to understanding of cellular metabolism in the context of metabolic disease and obesity. I initiated my studies in the field of diabetes and cell biology at the Albert Einstein College of Medicine in the laboratory of Dr. Lloyd Fricker, studying the role of carboxypeptidase E in obesity.  I continued my research at the Memorial Sloan Kettering Cancer Center studying the cell biological aspects of membrane transport in the laboratory of Dr. James Rothman and later, in the laboratory of Dr. Wolfhard Almers at the Vollum Institute, OHSU, where I began developing new interest in advanced fluorescent microscopy.  In 2009, I was recruited to the Oregon National Primate Research Center, where I developed new interest in adipose biology and established novel ex vivo tissue models using confocal microscopy.  In 2013, I received an NIA R21 award to study the role of high-fat diet and androgen deficiency, and have been a co-investigator on several other projects. I have recently developed new interest in understanding of the role of bone marrow adipose tissue, with a particular emphasis on the regulation of hematopoietic stem cell fate by bone marrow adipocytes.

Areas of interest

  • Obesity and adipocyte dysfunction
  • Sex hormones and metabolic disease
  • Metabolic memory (programing)
  • Western diet and hematopoietic stem cells
  • Bone marrow niche and hematopiesis
  • Quantitative microscopy of cellular niches

Education

  • M.D., Moscow Medical State University Russian Federation 1993
  • M.D., Pirogov Russian National Research Medical University, Moscow Russian Federation 1993
  • Ph.D., Albert Einstein College of Medicine New York United States 1998
  • Ph.D., Albert Einstein College of Medicine 1998

Honors and awards

  • Julius Marmur Award of the Sue Golding Graduate Division at Albert Einstein College for “Excellence in biomedical research”

Memberships and associations

  • Pacific Northwest Microscopy Society
  • Endocrine Society
  • Knight Cancer Institute

Publications

Selected publications

  • Cameron JL, Jain R, Rais M, White AE, Beer TM, Kievit P, Winters-Stone K, Messaoudi I, Varlamov O. (2016) Perpetuating effects of androgen deficiency on insulin resistance. International journal of obesity (2005). 40(12):1856-1863.

  • Chu M, Sampath H, Cahana DY, Kahl CA, Somwar R, Cornea A, Roberts CT Jr, Varlamov O. (2014) Spatiotemporal dynamics of triglyceride storage in unilocular adipocytes. Molecular biology of the cell. 25(25):4096-105.

  • Varlamov O, Chu MP, McGee WK, Cameron JL, O'Rourke RW, Meyer KA, Bishop CV, Stouffer RL, Roberts CT Jr.  (2013). Ovarian cycle-specific regulation of adipose tissue lipid storage by testosterone in female nonhuman primates. Endocrinology. 154(11):4126-35.

  • Varlamov O, White AE, Carroll JM, Bethea CL, Reddy A, Slayden O, O'Rourke RW, Roberts CT Jr. (2012). Androgen effects on adipose tissue architecture and function in nonhuman primates. Endocrinology. 153(7):3100-10.

  • Varlamov O., Fricker L.D., Furukawa H., Steiner D.F., Langley S.H., and Leiter E.H. (1997).  Beta-cell lines derived from transgenic CPEfat/CPEfat mice are defective in carboxypeptidase E and proinsulin processing. Endocrinology 138:4883-4892

  • Varlamov O. and Fricker D.L. (1998). Intracellular trafficking of metallocarboxypeptidase D in AtT-20 cells: localization to the trans-Golgi network and recycling from the cell surface. J. Cell Science 111:877-885

  • Eng F.J., Varlamov O, and Fricker L.D. (1999).  Sequences within the cytoplasmic domain of gp180/carboxypeptidase D mediate localization to the trans-Golgi network. Mol. Biol. Cell 10:35-46

  • Qian Y., Varlamov O., and Fricker L.D. (1999).  Glu300 of rat carboxypeptidase E is essential for enzymatic activity but not substrate binding or routing to the regulated secretory pathway. J. Biol. Chemistry 274:11582-6

  • Varlamov O., Wu F., Shields D., and Fricker L.D. (.1999).  Biosynthesis and packaging of carboxypeptidase D into nascent secretory vesicles in pituitary cell lines. J. Biol. Chemistry 274:14040-5

  • Varlamov O., Eng F.J., Novikova E.G., and Fricker L.D. (1999).Localization of metallocarboxypeptidase D in AtT-20 cells. Potential role in prohormone processing. J. Biol. Chemistry 274:14759-67

Publications

  • "Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack" Molecular Biology of the Cell April 2004
  • "NovyÇ� fluorogennyÇ� substrat karboksipeptidazy H--o-kumaroilfenilalanil-alanil-arginin." Bioorganicheskaya Khimiya April 1994
  • "Hypoxia-induced inflammatory cytokine secretion in human adipose tissue stromovascular cells" Diabetologia June 2011
  • "Detection of SNARE complexes with FRET using the tetracysteine system" BioTechniques February 2012
  • "The C-terminal region of carboxypeptidase E involved in membrane binding is distinct from the region involved with intracellular routing" Journal of Biological Chemistry March 15 1996
  • "Distinct SNARE complexes mediating membrane fusion in Golgi transport based on combinatorial specificity" Proceedings of the National Academy of Sciences of the United States of America April 16 2002
  • "Single-cell analysis of insulin-regulated fatty acid uptake in adipocytes" American Journal of Physiology - Renal Fluid and Electrolyte Physiology September 2010
  • "Selective control of SNARE recycling by golgi retention" FEBS Letters August 2 2013
  • "Sequences within the cytoplasmic domain of Gp180/carboxypeptidase D mediate localization to the trans-Golgi network" Molecular Biology of the Cell January 1999
  • "Sex-specific differences in lipid and glucose metabolism" Frontiers in Endocrinology  2014
  • "Ovarian cycle-specific regulation of adipose tissue lipid storage by testosterone in female nonhuman primates" Endocrinology November 1 2013
  • "Localization of metallocarboxypeptidase D in atT-20 cells" Journal of Biological Chemistry May 21 1999
  • "β-cell lines derived from transgenic Cpe(fat)/Cpe(fat) mice are defective in carboxypeptidase E and proinsulin processing" Endocrinology  1997
  • "Protein phosphatase 2A binds to the cytoplasmic tail of carboxypeptidase D and regulates post-trans-Golgi network trafficking" Journal of Cell Science  2001
  • "Autistic children display elevated urine levels of bovine casomorphin-7 immunoreactivity" Peptides  2014
  • "Androgen effects on adipose tissue architecture and function in nonhuman primates" Endocrinology July 2012
  • "Induced and spontaneous mutations at Ser202 of carboxypeptidase E" Journal of Biological Chemistry  1996
  • "Biosynthesis and packaging of carboxypeptidase D into nascent secretory vesicles in pituitary cell lines" Journal of Biological Chemistry May 14 1999
  • "i-SNAREs" Journal of Cell Biology January 2004
  • "Effect of carboxypeptidase E deficiency on progastrin processing and gastrin messenger ribonucleic acid expression in mice with the fat mutation" Endocrinology  1997
  • "Analysis of the carboxypeptidase D cytoplasmic domain" Journal of Cellular Biochemistry  2002
  • "A SNARE required for retrograde transport to the endoplasmic reticulum" Proceedings of the National Academy of Sciences of the United States of America August 19 2003
  • "Intracellular trafficking of metallocarboxypeptidase D in AtT-20 cells" Journal of Cell Science  1998
  • "Spatiotemporal dynamics of triglyceride storage in unilocular adipocytes" Molecular Biology of the Cell December 15 2014
  • "Glu300 of rat carboxypeptidase E is essential for enzymatic activity but not substrate binding or routing to the regulated secretory pathway" Journal of Biological Chemistry April 23 1999
  • "Carboxypeptidase Z is present in the regulated secretory pathway and extracellular matrix in cultured cells and in human tissues" Journal of Biological Chemistry February 18 2000
  • "Live-cell imaging demonstrates rapid cargo exchange between lipid droplets in adipocytes" FEBS Letters June 23 2011
  • "Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation" Proceedings of the National Academy of Sciences of the United States of America April 6 2004
  • "Real-time detection of snare complex assembly with fret using the tetracysteine system" Methods in molecular biology (Clifton, N.J.)  2014
  • "Dynamic transport of SNARE proteins in the Golgi apparatus" Proceedings of the National Academy of Sciences of the United States of America October 11 2005

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