Flow Cytometry Core Services

Facilities

We are located at the Oregon Health and Science University West Campus

505 N.W. 185th Avenue, Beaverton, OR  97006

The Flow Cytometry instruments are housed in three separate rooms of Building 1.

Although small refrigerators are available associated with each instrument, the available facilities do not include laboratory spaces or equipment for sample preparation (except for the Aria-II cell sorter, which is housed within a Biosafety Level 3 suite). 

Equipment

Becton Dickinson LSR-II

4 lasers, 18 color detectors

Laser 1:  UV, 355nm, (  )mW

3 associated detectors:  BUV395, BUV496/Hoechst, BUV737

Laser 2:  Violet, 405nm, (  )mW

7 associated detectors:  BV421/PacBl, BV510/AmCy, BV570, BV605, BV650, BV711, BV786

Laser 3:  Blue, 488nm, (  )mW

5 associated detectors:  FITC, PE, PE-TexasRed, PerCP-Cy5.5, PE-Cy7

Laser 4:  Red, 637, (  )mW

3 associated detectors:  APC, AlexaFluor700, APC-Cy7

Becton Dickinson LSR-II

4 lasers, 18 color detectors

Laser 1:  Violet, 405nm, (  )mW

8 associated detectors:  BV421/PacBl, BV510/AmCy, QD655, BV570/QD585, BV605/QD605, BV650/Q655, BV711/QD705, BV786/QD800

Laser 2:  Blue, 488nM, (  )mW

2 associated detectors:  FITC, PerCP-Cy5.5

Laser 3:  Green, 535nM, 150mW

5 associated detectors:  PE, PE-TexasRed, PE-Cy5, PE-Cy5.5, PE-Cy7

Laser 4:  Red, 637nm, 25mW

3 associated detectors:  APC, AlexaFluor700, APC-Cy7

Becton Dickinson FACS Aria-II

3 lasers, 15 color detectors

Laser 1:  Violet, 405nm, 100mW

7 associated detectors:  BV421/PacBl, BV510/AmCy, BV570, BV605, BV650, BV711, BV786

Laser 2:  Blue, 488nm, 50mW

5 associated detectors:  FITC, PE, PE-TexasRed, PerCP-Cy5.5, PE-Cy7

Laser 3:  Red, 640nm, 40mW

3 associated detectors:  APC, AlexaFluor700, APC-Cy7

Becton Dickinson FACSAria Fusion

4 lasers, 18 color detectors

Laser 1:  UV, 355nm, 60mW

3 associated detectors:  BUV395, BUV737, BUV805 (filter set for Hoechst)

Laser 2:  Violet, 405nm, 100mW

7 associated detectors:  BV421/PacBl, BV510/AmCy, BV570, BV605, BV650, BV711, BV786

Laser 3:  Blue, 488nm, 100mW

5 associated detectors:  FITC, PE, PE-TexasRed, PerCP-Cy5.5 PE-Cy7

Laser 4:  Red, 637, 140mW

3 associated detectors:  APC, AlexaFluor700, APC-Cy7

Services

The Flow Core offers four free training opportunities:
 

  • Flow 101:  a 2.5hr classroom session in the theory and basics of flow cytometry.  For novices-to-experienced users (the class can be easily tailored).  Offered 3-4 times/yr.
  • Flow 102:  a 2hr practicum session scheduled with the Director.  In this practicum, the User has real samples, and the Director goes through a detailed checklist of how to use the cytometer, from power-up to power-down.  A key product of this session is an expertly set up template for the type of samples the User brings.  This template can be re-used going forward.
  • Flow 103:  a 2-3hr occasionally offered classroom session in the use of either the FlowJo or Diva (or both) software packages for data analysis.
  • Flow 104:  a 2-4hr classroom+practicum session for users of the cell sorters.  These sessions build upon Flow101 and Flow102, and add the additional details of using a cell sorter, and safety with aerosolized biohazardous materials.  As necessary, the practicum includes special training to VGTI staff on safety practices in the BSL3 facility.
     

The Director of the Flow Core is available for free consultation, regarding experimental design, panel creation, reagent tittering, trouble-shooting, grantwork, and manuscript descriptions of cytometry work.

The Flow Core Assistant is available for rapid and free trouble-shooting of technical problems.  The Assistant can also collect samples for Users, on a fee-for-use basis (charged at the same hourly rate as the instrument).  The Assistant can run User samples on any of the instruments (analyzers or sorters), and can perform trickier tasks with greater expertise than an occasional User (e.g., single-cell sorting into plates).