Flow Cytometry Core Services
Location
OHSU West Campus / Oregon National Primate Research Center
505 N.W.185th Avenue
Vaccine and Gene Therapy Institute
West Campus Cytometers
Analyzers:
“LSR2A”: BD LSR-II / 4 lasers (UV/V/B/R) / 18 detector (3U/7V/5B/3R)
“LSR2B”: BD LSR-II / 4 lasers (V/B/G/R) / 18 detector (8V/2B/5G/3R)
“A528A”: BD A5 Symphony / 5 lasers (UV/V/B/YG/R) / 28 detector (8UV/7V/5B/5YG/3R)
Cell sorters:
“Aria-II”: BD Aria-II / 3 lasers (V/B/R) / 15 detector (7V/5B/3R) – Inside Biosafety Level 3 facility
“AriaFusion”: BD FACSAria Fusion / 4 lasers (UV/V/B/R) / 18 detectors (3U/7V/5B/3R) – inside biosafety cabinet
Free Flow Cytometry Classes
Flow101 (2.5hr classroom event) – Basic theory of flow cytometry, and introduction to technical details
Flow102 (2.0 one-on-one practicum) – Learning to use the Core analyzers
Flow103 (3hr+, multi-session one-on-one practicum) – Learning to use the Core cell sorters
Flow104 (2hr classroom event) – Introduction to FlowJo analysis software
Flow105 (1.5hr classroom event) – Titering antibody reagents
Flow106 (customized one-on-one or classroom event) – Designing cytometry staining panels
Rates
Rental costs (as of July 2022):
LSR analyzers: $65.10/hr
A528A analyzer: $80.48/hr
Aria-II sorter (in BSL3): $69.73/hr
AriaFusion sorter (in BSC): $75.24/hr
FlowCore Assistant: Hourly rate equal to the instrument used