Instruments on Marquam Hill
The ALM Core has a number of different imaging setups that excel at different applications. Below is an overview of instruments located on Marquam Hill in LBRB.
Laser Scanning Confocals
Our laser-scanning confocal microscopes are equipped with objectives that have high numerical apertures for optimal multi-color imaging and optical sectioning.
Zeiss LSM 980 with Airyscan 2. Laser scanning confocal microscope with 3-channel spectral detector for maximum flexibility in emission wavelength selection. The system also features the new Airyscan 2 detector with 4Y mode for faster high resolution imaging.
Zeiss LSM 900 with Airyscan 2. Laser scanning confocal microscope on an inverted stand with variable beam splitter dichroics for maximum flexibility in detection and the new Airyscan 2 detector with 4Y mode for faster high resolution imaging.
Spinning Disk Confocal
Yokogawa CSU-W1 on a Nikon TiE. Spinning disk confocal on an inverted stand with high-content analysis (HCA) capabilities. Spinning disk confocal microscopy outperforms laser-scanning microscopy for live-cell imaging. This setup accepts mutli-well plates for image-based screening assays.
Andor BC43 benchtop spinning disk confocal for fixed sample imaging. This system has four channels and can tile larger areas. Spinning disk technology is fast and more affordable compared to traditional laser scanning confocal technology.
Widefield Microscopy
Zeiss Apotome.2 on an Axio Imager. Our most economical solution for imaging fluorescence and colorimetric stains of fixed samples. System is on an upright stand and uses Apotome.2 for grid-based optical sectioning and a color camera for high resolution color imaging.
Zeiss Celldiscoverer 7. Automated time lapse imaging of multi-well plates at high resolution. Definite Focus 2 and Software Autofocus functions keep samples in focus over long periods of time.
Sartorius IncuCyte S3. Imaging device inside a tissue-culture incubator. Designed for following cell growth and differentiation over days, quantifying cell migration, and angiogenesis and neurite outgrowth over time.
Super Resolution Microscopy
Zeiss Elyra 7 with lattice SIM². The Elyra 7 is Zeiss' new and improved structured illumination system for super resolution imaging on an inverted stand.
Multi-Photon Excitation
Zeiss LSM 980 NLO. Multi-photon microscope on an upright imaging stand. This setup is designed for intravital imaging of live rodents.