Amanda K McCullough, Ph.D.

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Phone:: 503 494-9958

Background

Dr. McCullough received her doctoral degree in cellular and molecular biology from the University of Vermont. She completed postdoctoral training at Oregon Health & Science University and the University of Texas Medical Branch in Galveston, Texas. She was an Assistant Professor in the Department of Human Biological Chemistry and Genetics at the University of Texas before joining Center for Research on Occupational and Environmental Toxicology (CROET) at OHSU in August 2003.

Research in the McCullough laboratory is focused on the biochemical mechanisms of DNA repair systems and the regulation and roles of DNA repair in cellular responses to environmental stress. Ultimately, we are interested in correlating alterations in these systems with human cancers, aging and other disease states. Currently our research is focused on: 1) cellular pathways for the tolerance and repair of DNA-protein crosslinks; 2) biochemical mechanisms and therapeutic applications of ultraviolet (UV) light-induced DNA damage-specific glycosylases; and 3) mechanisms and regulation of human oxidative DNA damage-specific repair and how defects in these pathways contribute to colorectal cancers and metabolic disease.

Selected Publications

"Regulation of DNA glycosylases and their role in limiting disease," Free Radical Research (Vol: 46, Issue: 4, Page 460-478) - 2012

"Formaldehyde-induced genome instability is suppressed by an XPF-dependent pathway," DNA Repair (Vol: 11, Issue: 3, Page 236-246) - 2012

"Modulation of the processive abasic site lyase activity of a pyrimidine dimer glycosylase," DNA Repair (Vol: 10, Issue: 10, Page 1014-1022) - 2011

"Î³-Hydroxy-1, N ²-propano-2â€²-deoxyguanosine DNA adduct conjugates the N-terminal amine of the KWKK peptide via a carbinolamine linkage," Chemical Research in Toxicology (Vol: 24, Issue: 7, Page 1123-1133) - 2011

"Variable penetrance of metabolic phenotypes and development of high-fat diet-induced adiposity in NEIL1-deficient mice.," American journal of physiology. Endocrinology and metabolism (Vol: 300, Issue: 4, Page E724-734) - 2011

"TAT-mediated delivery of a DNA repair enzyme to skin cells rapidly initiates repair of UV-induced DNA damage," Journal of Investigative Dermatology (Vol: 131, Issue: 3, Page 753-761) - 2011

"Minor groove orientation of the KWKK peptide tethered via the N-terminal amine to the acrolein-derived 1, N ²-Î³- hydroxypropanodeoxyguanosine lesion with a trimethylene linkage," Biochemistry (Vol: 49, Issue: 29, Page 6155-6164) - 2010

"Modulation of UvrD helicase activity by covalent DNA-protein cross-links," Journal of Biological Chemistry (Vol: 285, Issue: 28, Page 21313-21322) - 2010

"Cellular pathways for DNA repair and damage tolerance of formaldehyde-induced DNA-protein crosslinks," DNA Repair (Vol: 8, Issue: 10, Page 1207-1214) - 2009

"The basal levels of 8-oxoG and other oxidative modifications in intact mitochondrial DNA are low even in repair-deficient (Ogg1^-/-/Csb^-/-) mice," Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis (Vol: 625, Issue: 1-2, Page 155-163) - 2007

"Human polymorphic variants of the NEIL1 DNA glycosylase," Journal of Biological Chemistry (Vol: 282, Issue: 21, Page 15790-15798) - 2007

"Uncoupling of nucleotide flipping and DNA bending by the T4 pyrimidine dimer DNA glycosylase," Biochemistry (Vol: 45, Issue: 47, Page 14192-14200) - 2006

"Structure of T4 Pyrimidine Dimer Glycosylase in a Reduced Imine Covalent Complex with Abasic Site-containing DNA," Journal of Molecular Biology (Vol: 362, Issue: 2, Page 241-258) - 2006

"The metabolic syndrome resulting from a knockout of the NEIL1 DNA glycosylase," Proceedings of the National Academy of Sciences of the United States of America (Vol: 103, Issue: 6, Page 1864-1869) - 2006

"Reaction intermediates in the catalytic mechanism of Escherichia coli MutY DNA glycosylase," Journal of Biological Chemistry (Vol: 279, Issue: 45, Page 46930-46939) - 2004

"Identification of critical residues required for the mutation avoidance function of human MutY (hMYH) and implications in colorectal cancer," Cancer Letters (Vol: 205, Issue: 1, Page 89-95) - 2004

"Chlorella virus pyrimidine dimer glycosylase excises ultraviolet radiation- and hydroxyl radical-induced products 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from DNA," Photochemistry and Photobiology (Vol: 75, Issue: 2, Page 85-91) - 2002

"hMYH cell cycle-dependent expression, subcellular localization and association with replication foci: Evidence suggesting replication-coupled repair of adenine:8-oxoguanine mispairs," Nucleic Acids Research (Vol: 29, Issue: 13, Page 2802-2809) - 2001

"The reaction mechanism of DNA glycosylase/AP lyases at abasic sites," Biochemistry (Vol: 40, Issue: 2, Page 561-568) - 2001

"AP lyases and dRPases: Commonality of mechanism," Mutation Research - DNA Repair (Vol: 459, Issue: 1, Page 43-53) - 2000

"Intron conservation in a UV-specific DNA repair gene encoded by chlorella viruses," Journal of Molecular Evolution (Vol: 50, Issue: 1, Page 82-92) - 2000

"Initiation of base excision repair: Glycosylase mechanisms and structures," Annual Review of Biochemistry (Vol: 68, Issue: , Page 255-285) - 1999

"Characterization of a novel cis-syn and trans-syn-II pyrimidine dimer glycosylase/AP lyase from a eukaryotic algal virus, Paramecium bursaria chlorella virus-1," Journal of Biological Chemistry (Vol: 273, Issue: 21, Page 13136-13142) - 1998

"Chlorella virus PBCV-1 encodes a homolog of the bacteriophage T4 UV damage repair gene denV," Applied and Environmental Microbiology (Vol: 63, Issue: 4, Page 1551-1556) - 1997

"The role of base flipping in damage recognition and catalysis by T4 endonuclease V," Journal of Biological Chemistry (Vol: 272, Issue: 43, Page 27210-27217) - 1997