Maureen Hoatlin, Ph.D.

Email:: click here
Phone:: 503 494-1123; Lab Page: http://openwetware.org/wiki/Hoatlin_Lab

Background

We are interested in understanding how cells maintain genomic stability. One of the mechanisms that regulates this critical process is defective in Fanconi anemia (FA), a genetic model for human susceptibility to cancer. FA is a rare but devastating multi-gene disease thought to have an underlying defect in DNA interstrand crosslink repair.

Our lab pioneered the use of cell-free assays for FA proteins in extracts from Xenopus eggs. These extracts allow analysis of FA protein function and post-translational modifications in a context that is permissive for naturally-regulated DNA synthesis. The recruitment of Fanconi proteins to chromatin in S-phase is providing us with a biochemical platform for elucidating the molecular function of the Fanconi proteins during the DNA damage response.

Selected Publications

"A Ubiquitin-Binding Protein, FAAP20, Links RNF8-Mediated Ubiquitination to the Fanconi Anemia DNA Repair Network," Molecular Cell (Vol: 47, Issue: 1, Page 61-75) - 2012

"BRCAness profile of sporadic ovarian cancer predicts disease recurrence," PLoS ONE (Vol: 7, Issue: 1, Page ) - 2012

"Targeting the fanconi anemia pathway to identify tailored anticancer therapeutics," Anemia (Vol: 2012, Issue: , Page ) - 2012

"Evidence for complete epistasis of null mutations in murine Fanconi anemia genes Fanca and Fancg," DNA Repair (Vol: 10, Issue: 12, Page 1252-1261) - 2011

"A new FAN of the stalled replication fork," Cell Cycle (Vol: 9, Issue: 21, Page 4261-4262) - 2010

"A Histone-Fold Complex and FANCM FormÂ a Conserved DNA-Remodeling Complex to Maintain Genome Stability," Molecular Cell (Vol: 37, Issue: 6, Page 865-878) - 2010

"Structure-specific recognition protein 1 facilitates microtubule growth and bundling required for mitosis," Molecular and Cellular Biology (Vol: 30, Issue: 4, Page 935-947) - 2010

"Monoketone analogs of curcumin, a new class of Fanconi anemia pathway inhibitors," Molecular Cancer (Vol: 8, Issue: , Page ) - 2009

"Transcriptional activation by the Mixl1 homeodomain protein in differentiating mouse embryonic stem cells," Stem Cells (Vol: 27, Issue: 12, Page 2884-2895) - 2009

"The Fanconi anemia protein FANCM is controlled by FANCD2 and the ATR/ATM pathways," Journal of Biological Chemistry (Vol: 284, Issue: 38, Page 25560-25568) - 2009

"A novel cell-free screen identifies a potent inhibitor of the fanconi anemia pathway," International Journal of Cancer (Vol: 124, Issue: 4, Page 783-792) - 2009

"Fanconi anemia proteins stabilize replication forks," DNA Repair (Vol: 7, Issue: 12, Page 1973-1981) - 2008

"RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability," Genes and Development (Vol: 22, Issue: 20, Page 2843-2855) - 2008

"FANCG promotes formation of a newly identified protein complex containing BRCA2, FANCD2 and XRCC3," Oncogene (Vol: 27, Issue: 26, Page 3641-3652) - 2008

"Identification, developmental expression and regulation of the Xenopus ortholog of human FANCG/XRCC9," Genes to Cells (Vol: 12, Issue: 7, Page 841-851) - 2007

"DNA structure-induced recruitment and activation of the Fanconi anemia pathway protein FANCD2," Molecular and Cellular Biology (Vol: 27, Issue: 12, Page 4283-4292) - 2007

"FAAP100 is essential for activation of the Fanconi anemia-associated DNA damage response pathway," EMBO Journal (Vol: 26, Issue: 8, Page 2104-2114) - 2007

"The nuclear accumulation of the Fanconi anemia protein FANCE depends on FANCC," DNA Repair (Vol: 5, Issue: 5, Page 556-565) - 2006

"Fanconi anemia proteins are required to prevent accumulation of replication-associated DNA double-strand breaks," Molecular and Cellular Biology (Vol: 26, Issue: 2, Page 425-437) - 2006

"A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M," Nature Genetics (Vol: 37, Issue: 9, Page 958-963) - 2005

"BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity," EMBO Journal (Vol: 24, Issue: 7, Page 1465-1476) - 2005

"X-linked inheritance of Fanconi anemia complementation group B," Nature Genetics (Vol: 36, Issue: 11, Page 1219-1224) - 2004

"The Fanconi anemia gene product FANCF is a flexible adaptor protein," Journal of Biological Chemistry (Vol: 279, Issue: 38, Page 39421-39430) - 2004

"Heterogeneity in Fanconi anemia: Evidence for 2 new genetic subtypes," Blood (Vol: 103, Issue: 7, Page 2498-2503) - 2004

"FANCD2 protein is expressed in proliferating cells of human tissues that are cancer-prone in Fanconi anaemia," Journal of Pathology (Vol: 201, Issue: 2, Page 198-203) - 2003