Transgenic Mouse Models Core laboratory (TMM) is a structural unit of the OHSU Shared Resource Program and is supported by this program and the Knight Cancer Institute at OHSU. The Transgenic Core was established within OHSU in 1994 in response to a growing interest and need for this important technology of many scientists and physicians among different faculties of OHSU. There has been exponential growth of transgenic mouse projects at OHSU with over two hundred fifty unique transgenic and knockout mouse lines produced during the past 17 years. Moreover, the genetically modified mice generated by OHSU's Transgenic Core are successfully used by investigators within the USA and other countries. Currently the TMM supports 27 grants from NIH and other funding agencies. Furthermore, TMM has more than 30 OHSU users from Oregon Stem Center, Pape' Pediatric Research Institute, Vollum Institute, Knight Cancer Institute, Department of Physiology & Pharmacology, Oregon Hearing Research Center, Department of Molecular & Medical Genetics, Department of Molecular Microbiology & Immunology, Center for Research in Occup. & Environ. Toxicology, Department of Otolaryngology, Department of Cell and Developmental Biology, Department of Behavioral Neuroscience and Division of Pulmonary & Critical Care Medicine. Furthermore, TMM supports external customers from Portland Veterans Affairs Medical Center, Shriners Hospital for Children and Bend Research, Inc.
To improve techniques and accumulate new knowledge in Transgenic Technology the TMM staff is regularly participating in courses, workshops and conferences organized by the Jackson Laboratories, International Society for Transgenic Technologies (ISTT) and the Cold Spring Harbor Laboratory.
According to the long term developmental strategy of OHSU the construction of the Transgenic Core lab was completely finished and we relocated to the Biomedical Research Building (BRB) on July 2011. This allows the molecular biology component of the Core to have a specialized area that occupies one standard lab bay. While other important components such as ES tissue culture and embryo microinjection laboratories are now located in a specific pathogen free (SPF) barrier area. The animal housing rooms for the Transgenic Core are now adjacent to the Department of Comparative Medicine (DCM). The site of the newly constructed Transgenic Core, on the BRB level 1 with close proximity to DCM, is exceptionally convenient as it provides a controlled sterile environment that is easily maintained while facilitating improved transportation of newly generated mice to our OHSU customers from a better centralized location.
New lab space of TMM; Summer, 2011. (A) Molecular laboratory. (B) Embryo microsurgery room. (C) ES cell culture room. (D) SPF mouse room. Mice are housed within individually ventilated cages (IVC). (E) All animal manipulations are performed inside of Biosafety Cabinets under SPF conditions.
Our Current Partners
Department of Comparative Medicine (DCM) provides professional and technical services for laboratory animal care, veterinary medical care and control for our animals. Moreover, DCM cooperates with TgMM to rederivate contaminated mouse strains and to maintain healthy mouse colonies in OHSU.
Research Cytogenetics Core Laboratory provides high quality metaphase and interphase cytogenetic data to the OHSU research community. Services are also available to non-profit and commercial investigators located in Oregon and elsewhere.
DNA Services Core performs DNA sequencing, genomic analysis and DNA (oligonucleotide) synthesis. Services are also available to non-profit and commercial investigators located in Oregon and elsewhere.
Charles River laboratory is a vendor of inbred and genetically modified mice. Additionally Charles River performs diagnostic services to prevent a contamination of animal colonies by different pathogens.
International Knockout Mouse Consortium (IKMC) consists of American, Canadian, and European members and produces genetically modified ES cells and mice. Currently the members of the IKMC are working together to mutate all protein-coding genes in the mouse using a combination of gene trapping and gene targeting particularly in C57BL/6 mouse embryonic stem (ES) cells.
Mutant Mouse Regional Resource Centers (MMRRC) is an American vendor of genetically engineered mouse strains and mouse ES cell lines.
European Conditional Mouse Mutagenesis Program (EUCOMM) is a European member of IKMC, the producer of genetically modified ES cells and mice.