OHSU

Massively Parallel Sequencing Shared Resource

The OHSU Massively Parallel Sequencing Shared Resource functions as a full service genomics facility serving research scientists and clinicians with a full range of applications based upon massively parallel sequencing protocols.

About

The OHSU Massively Parallel Sequencing Shared Resource (MPSSR) was founded in 2009 to provide high quality, cost efficient short read sequencing services to help investigators examine the genetic profiles that underlie disease predisposition and to explore the fundamental operations of biological systems. In addition to sequencing services, the MPSSR offers a variety of library construction services - including genome resequencing with sequence capture and transcriptome analysis. Contact us about your proposed work to see how we can assist you.

We have recently added strand-specific sequencing of mRNA using ScriptSeq to our list of standard library protocols.

Funding for the Illumina GAIIx sequencer was provided by a generous grant from the M.J. Murdock Charitable Trust. Funding for our upgrade to the HiSeq 2000 was provided by OHSU.

The MPSSR receives support from the Knight Cancer Institute.

MPSSR Core Informational Flyer

Quick Links and Quick Information

Quick Links

  • Useful References - papers addressing various topics that relate to short read sequencing
  • Initiate a project using the MPSSR iLab page: iLab

Major Equipment

  • Illumina HiSeq 2000 Sequencer
  • Illumina cBot
  • Covaris S2 sonicator.
  • 16-core Dell Server with 41 Tb storage
  • ABI StepOne Real Time PCR System

Sequencing Human Samples

The sequence of DNA is a unique identifier that cannot be fully anonymous.  Because of this, all samples submitted to the MPSSR that have been derived from human subjects MUST be coded so that the MPSSR cannot directly identify the subject and MUST be accompanied by IRB approval.

Attribution

MPSSR Acknowledgement

When presenting and publishing results from projects processed by the Massively Parallel Sequencing Shared Resource, inclusion of the following statement is appreciated:

Short read sequencing assays were performed by the OHSU Massively Parallel Sequencing Shared Resource.

Contact the MPSSR director to report your publication for inclusion on our website!

External Users

The MPSSR is again accepting samples from outside the Oregon university system. We have a new contract that needs to be reviewed and signed by both parties before work can begin. We can also provide the required OHSU tax identification information. Please note that we are not accepting samples that require us to bill foreign entities.