OHSU

Resources

The IVIS Spectrum at the IOIC contains an optimized set of high efficiency filters and spectral un-mixing algorithms for detecting luminescent or fluorescent reporters across the blue to near infrared wavelength region.  The IVIS Spectrum is capable of either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources.  Fluorescent 3-D tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images.

The instrument is equipped with 12 narrow band excitation filters (35 nm bandwidth) and 24 narrow band emission filters (20 nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and unmixing algorithms. The spectral unmixing tools also allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Imaging platforms for either rat or mouse imaging have species-appropriate nosecones for inhaled isofluorane anesthesia which is provided through an integrated delivery system.  The facility also contains a refrigerator for materials storage and disposable equipment for injection, depiliation, eye protection, and warm environment recovery.  The core facility does not supply fluorophores, luminescent substrates, or injectable anesthetics. 

A Quick Reference guide to system use, suggested algorithms for luciferin preparation and luciferase detection, and a fluorescent probe reference that describes the excitation/emission spectral peaks are available online and onsite.