Welcome to the in vivo optical imaging core (IOIC), co-sponsored by the Knight Cancer Center and the Division of Cardiovascular Medicine at OHSU. This equipment resource is available to researchers at OHSU for the non-invasive imaging of fluorescent probes and cells, and bioluminescent reporters.
About the Equipment
The IOIC houses an IVIS Spectrum biophotonics imaging system (Caliper Life Sciences) that can non-invasively detect the spatial distribution of optical energy in anesthetized animals. The system is equipped with an array of narrowband excitation and emission filters across the blue to near infra-red wavelength spectrum to provide high-efficiency photon information. Single channel optical intensity information can be quantified with spectral unmixing software and algorithms to correct for attenuation. Information is can be spatially as 3-D tomograms or superimposed on a broad spectrum "white" light (structured) images. Images can also be exported for interlacing with other medical imaging data (CT, MR).
Common applications of this system include the tracking of ex vivo labeled cells, non-invasive molecular imaging of biologic/pathologic process with targeted probes, evaluation of reporter gene expression, and biodistribution of macromolecules. Please refer to the for examples of disease-specific applications of optical imaging.
About the Facility
The IOIC is located in Cardiac Imaging Laboratory on the first floor of the Biomedical Research Building (BRB). The laboratory was established through a Shared Instrument Grant (PI: Michael Deininger) but is maintained through user fees. The core facility includes the IVIS Spectrum optical imaging camera housed in a light-tight housing, rat and mouse imaging platforms for multi-subject simultaneous acquisition, an integrated inhaled anesthesia system, and advanced analysis software.
A key component of the IOIC is the ability to operate either independently or under the guidance and supervision of core personnel. Independent use of the system requires that investigators undergo training that is offered every 2 months or on an ad hoc basis. All analysis will be charged on an hourly basis and is graduated according to the level of service (independent or supervised).
Access will be limited to normal business hours (9:00 a.m. to 5:00 p.m.)