Immunohistochemistry

The Histopathology Shared Resource provides Immuhistochemistry services which include:

• Testing and titering new antibodies, both monoclonal and polyclonal. The latter should be either a purified IgG fraction or affinity-purified. (Straight serum is not suitable for immunostaining).
• Optimization of staining to enhance detection and reduce background.
• Epitope retrieval techniques (pre-treatment with heat and/or proteases)
• Specialized secondary detection reagents
• Avidin/biotin blocking steps
• Double- and triple-antibody stains using multiple chromagens.

For more information on Immuhistochemistry services, visit our Contact page.